Is Continuous Treatment with Transforming Growth Factor-Beta Necessary to Induce Chondrogenic Differentiation in Mesenchymal Stem Cells?

Hye-Joung Kim; Young-Ju Kim; Gun-Il Im
June 2009
Cells Tissues Organs;2009, Vol. 190 Issue 1, p1
Academic Journal
This study was conducted to determine whether short-term administration of transforming growth factor (TGF)-β would be as effective for inducing chondrogenesis in human mesenchymal stem cells (hMSCs) as continuous treatment. Four groups of hMSCs were cultured in a monolayer for 3 days followed by a pellet culture for 3 weeks under various conditions: group A, the control group, no growth factors treated; group B, 5 ng/ml of TGF-β2 was treated for 3 days in monolayer culture; group C, 5 ng/ml of TGF-β2 was treated for 3 days in a monolayer culture and the initial 3 days of pellet culture; group D, 5 ng/ml of TGF-β2 was treated for 3 days in a monolayer culture and the initial 10 days of pellet culture; group E, 5 ng/ml of TGF-β2 was continuously treated throughout the culture period. Glycosaminoglycan contents significantly increased in group E only. Real-time PCR indicated that expression of Sox-9, type II collagen, type II procollagen B and type X collagen increased with longer duration of TGF-β2 treatment. The histological findings showed that longer duration of TGF-β2 treatment led to significantly better quality of chondrogenesis. This study demonstrated that longer duration of TGF-β treatment is necessary for effective chondrogenesis in hMSCs from bone marrow. Copyright © 2008 S. Karger AG, Basel


Related Articles

  • Substrate and strain alter the muscle-derived mesenchymal stem cell secretome to promote myogenesis. De Lisio, Michael; Jensen, Tor; Sukiennik, Richard A.; Huntsman, Heather D.; Boppart, Marni // Stem Cell Research & Therapy;2014, Vol. 5 Issue 3, p1 

    Introduction Mesenchymal stem cells (MSCs) reside in a variety of tissues and provide a stromal role in regulating progenitor cell function. Current studies focus on identifying the specific factors in the niche that can alter the MSC secretome, ultimately determining the effectiveness and...

  • Mesenchymal stromal cells: a novel and effective strategy for facilitating engraftment and accelerating hematopoietic recovery after transplantation? Bernardo, M E; Cometa, A M; Locatelli, F // Bone Marrow Transplantation;Mar2012, Vol. 47 Issue 3, p323 

    MSCs are multipotent cells that can be isolated from several human tissues and expanded ex vivo for clinical use. They comprise a heterogeneous population of cells, which, through production of growth factors, cell-to-cell interactions and secretion of matrix proteins, has a role in the...

  • Regulation of endodermal differentiation of human embryonic stem cells through integrin-ECM interactions. Brafman, D A; Phung, C; Kumar, N; Willert, K // Cell Death & Differentiation;Mar2013, Vol. 20 Issue 3, p369 

    Many cellular responses during development are regulated by interactions between integrin receptors and extracellular matrix proteins (ECMPs). Although the majority of recent studies in human embryonic stem cell (hESC) differentiation have focused on the role of growth factors, such as FGF,...

  • Delivery of Platelet-Derived Growth Factor as a Chemotactic Factor for Mesenchymal Stem Cells by Bone-Mimetic Electrospun Scaffolds. Phipps, Matthew C.; Yuanyuan Xu; Bellis, Susan L. // PLoS ONE;Jul2012, Vol. 7 Issue 7, p1 

    The recruitment of mesenchymal stem cells (MSCs) is a vital step in the bone healing process, and hence the functionalization of osteogenic biomaterials with chemotactic factors constitutes an important effort in the tissue engineering field. Previously we determined that bone-mimetic...

  • Growth Factor Priming Differentially Modulates Components of the Extracellular Matrix Proteome in Chondrocytes and Synovium-Derived Stem Cells. Alegre-Aguarón, Elena; Sampat, Sonal R.; Xiong, Jennifer C.; Colligan, Ryan M.; Bulinski, J. Chloë; Cook, James L.; Ateshian, Gerard A.; Brown, Lewis M.; Hung, Clark T. // PLoS ONE;Feb2014, Vol. 9 Issue 2, p1 

    To make progress in cartilage repair it is essential to optimize protocols for two-dimensional cell expansion. Chondrocytes and SDSCs are promising cell sources for cartilage repair. We previously observed that priming with a specific growth factor cocktail (1 ng/mL transforming growth...

  • The FoxO code. Calnan, D. R.; Brunet, A. // Oncogene;4/7/2008, Vol. 27 Issue 16, p2276 

    The FoxO family of Forkhead transcription factors plays an important role in longevity and tumor suppression by upregulating target genes involved in stress resistance, metabolism, cell cycle arrest and apoptosis. FoxO transcription factors translate a variety of environmental stimuli, including...

  • Basic FGF and suppression of BMP signaling sustain undifferentiated proliferation of human ES cells. Ren-He Xu; Peck, Ruthann M.; Li, Dong S.; Xuezhu Feng; Ludwig, Tenneille; Thomson, James A. // Nature Methods;Mar2005, Vol. 2 Issue 3, p185 

    Human embryonic stem cells (hESCs) are routinely cultured on fibroblast feeder layers or in fibroblast-conditioned medium (CM). Bone morphogenetic proteins (BMPs) have previously been shown to induce hESC differentiation, in apparent contrast to mouse embryonic stem (ES) cells, in which BMP4...

  • TGF-β signalling is mediated by two autonomously functioning TβRI:TβRII pairs. Huang, Tao; David, Laurent; Mendoza, Valentín; Yang, Yong; Villarreal, Maria; De, Keya; Sun, LuZhe; Fang, Xiaohong; López-Casillas, Fernando; Wrana, Jeffrey L; Hinck, Andrew P // EMBO Journal;4/6/2011, Vol. 30 Issue 7, p1263 

    Transforming growth factor (TGF)-βs are dimeric polypeptides that have vital roles in regulating cell growth and differentiation. They signal by assembling a receptor heterotetramer composed of two TβRI:TβRII heterodimers. To investigate whether the two heterodimers bind and signal...

  • CCN2, the connective tissue growth factor. Luft, Friedrich C. // Journal of Molecular Medicine;Jan2008, Vol. 86 Issue 1, p1 

    The article presents information about the connective tissue growth factor which is known as the CCN2. The importance of the role of extracellular matrix proteins in regulation of response and signaling control to injury, particularly in terms of scarring, is discussed. In relation, a study was...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics