TITLE

High-temperature liquid chromatography of steroids on a bonded hybrid column

AUTHOR(S)
Al-Khateeb, Lateefa A.; Smith, Roger M.
PUB. DATE
July 2009
SOURCE
Analytical & Bioanalytical Chemistry;Jul2009, Vol. 394 Issue 5, p1255
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A hybrid stationary phase, XTerra MS C18, has been evaluated for the high-temperature reversed-phase liquid chromatography of selected hydrophobic steroids. The effects on the retention and efficiency at temperatures up to 130°C and eluent compositions from methanol–water mixtures to superheated water were studied. The thermodynamic data of the separations were determined. It was shown that increasing the temperature enabled the percentage of methanol to be reduced. High mobile-phase flow rates could be used, but for these non-polar analytes, the retention times with superheated water as the eluent were still high.
ACCESSION #
41328800

 

Related Articles

  • The Use of Complimentary Stationary Phases and 2-Dimensional HPLC for the Separation of the Synthesis and Degradants of Tipredane (INN). Hulse, Jennifer; Euerby, Melvin R.; Taylor, Peter; Bassindale, Alan // LabAsia;Nov2014, Vol. 40 Issue 7, p10 

    The article examines how columns of complimentary chromatographic selectivity can be identified by stationary phase characterization in a two-dimensional liquid chromatography cutting method to separate a steroid and its degradants. It states that the separation mechanism of two C17...

  • Stationary Phases for High-Temperature Liquid Chromatography. Yu Yang // LC-GC Europe;Jun2003, Vol. 16 Issue 6a, p37 

    Examines the use of stationary phases at temperatures considerably higher than room temperature. Use of ristocetin A chiral stationary phase in studying the effect of temperature on the retention of several chiral compounds; Investigation of the use of porous graphitic carbon on...

  • The Thermally Tuned Tandem Column Approach to Optimizing Selectivity in HPLC. Yun Mao; Carr, Peter W. // LC-GC North America;Jun2003 Supplement, Vol. 21 Issue 6, p69 

    Describes the thermally tuned tandem column concept for the optimization of selectivity in liquid chromatography by effectively continuously adjusting the stationary phase. Serial connection of two columns with distinctly different chromatographic selectivities; Comparison of thermally tuned...

  • Monolithic Columns: The New Era in the Analysis of Organic Compounds by Liquid Chromatographic Techniques. Karageorgou, Eftichia G.; Samanidou, Victoria F. // Current Organic Chemistry;2010, Vol. 14 Issue 19, p2310 

    No abstract available.

  • LC and UV Methods for Lamotrigine Determination in Pharmaceutical Formulation. Martins, Magda T.; Paim, Clésio S.; Steppe, Martin // Chromatography Research International;2011, p1 

    Liquid chromatography (LC) and ultraviolet spectrophotometric (UV) methods for lamotrigine (LTG) determination were validated. The LC separation was achieved on an ACE RP-18 as stationary phase and 0.3% triethylamine in water (v/v) pH 4.0 : methanol (62 : 38, v/v) as mobile phase. Detection was...

  • 2-Minute Steroid Analysis Using a 1.9μm Pinnacle DB Biphenyl UHPLC Column. Lake, Rick; Wittrig, Becky // LC-GC North America;Feb2008 Applications Notebook, Vol. 26, p51 

    UHPLC, through the use <2 μm particle size columns, maximizes efficiency, but stationary phase selectivity should also be considered to fully optimize separations. Here we demonstrate an enhanced separation of steroids using a 1.9 μm Pinnacle™ DB Biphenyl column. This column combines...

  • Elevated Temperatures in Liquid Chromatography, Part III: A Closer Look at the van 't Hoff Equation. Jensen, David S.; Linford, Matthew R.; Teutenberg, Thorsten; Clark, Jody // LC-GC North America;Dec2012, Vol. 30 Issue 12, p1052 

    The article discusses various aspects of the utilization of elevated temperature approach in liquid chromatographic (LC) techniques. The usefulness and relevance of the Van't Hoff chemical equation which relates changes in temperature factor with other thermodynamics factor for data analysis in...

  • The Cleaning and Regeneration of Reversed-Phase HPLC Columns. Majors, Ronald E. // LC-GC North America;Jan2003, Vol. 21 Issue 1, p19 

    Discusses the cleaning and regeneration of reversed-phase high performance liquid chromatography (HPLC) columns. Use of the reversed-phase chromatography technique in HPLC; Hydrophobic nature of stationary phases used in reversed-phase chromatography; Separation of analytes by the degree of...

  • Honoring Readers.  // LC-GC North America;Sep2003, Vol. 21 Issue 9, p888 

    Responds to readers' questions on liquid chromatography troubleshooting. Use of the column plate number to reflect the status of a column; Behavior of a peak that is excluded from the stationary phase.

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics