Phosphoester hydrolysis by cerium(IV)-thiacalix[4]arene complexes and its application to immunoassay

Matsumiya, Hiroaki; Nakamura, Hiroko; Hiraide, Masataka
July 2009
Analytical & Bioanalytical Chemistry;Jul2009, Vol. 394 Issue 5, p1471
Academic Journal
Thiacalix[4]arenetetrasulfonate was treated with Ce(IV) in water at pH 9.5 to give novel phosphoester-hydrolyzing complexes. The dinuclear Ce(IV) complex promoted the hydrolysis of p-nitrophenyl phosphate with a turnover frequency of 6.8 h−1 at 50 °C, showing fourfold higher activity than the mononuclear complex. The dinuclear complex was readily immobilized onto an antibody by simply mixing them in water, hence its phosphatase-like activity was applied to the color-developing reaction in immunoassay. The model assay using an antibody labeled with the dinuclear complex allowed the detection of as little as 10 ng mL−1 of a tumor marker, Bence–Jones protein, in a 96-well microtiter plate format. Analysis of urine for Bence–Jones protein was performed by the proposed method.


Related Articles

  • Spectral analysis and catalytic activity in the hydrolysis of the phosphate ester by aza-crown ether cerium (III) complex. Bingying Jiang; Shulan Cai; Famei Feng; Jiaqing Xie // Journal of Chemical & Pharmaceutical Research;2014, Vol. 6 Issue 3, p1520 

    An aza-crown ether ligand, 4,7,13,16-tetraethoxycarbonylmethyl-1,10-dioxa-4,7,13,16-tetraaza-18-C-6, was synthesized and characterized in this work. The binary complex containing cerium ion (III) and the ligand was determined by fluorescence technique and then used as catalyst in the hydrolysis...

  • The hook effect: a need for constant vigilance. Jassam, N; Jones, C M; Briscoe, T; Horner, J H // Annals of Clinical Biochemistry;Jul2006, Vol. 43 Issue 4, p314 

    The hook effect is a well-recognized problem that can occur in assays of most tumour markers, including alpha feto-protein (AFP). We present a case of hepatoblastoma in a baby. The diagnosis was delayed as a result of unrecognized 'hooking' of a very high AFP concentration in the automated...

  • Distinct Metal Ion Requirements for the Phosphomonoesterase and Phosphodiesterase Activities of Calf Intestinal Alkaline Phosphatase. Igunnu, Adedoyin; Osalaye, Dunsin S.; Olorunsogo, Olufunso O.; Malomo, Sylvia O.; Olorunniji, Femi J. // Open Biochemistry Journal;2011, Vol. 5, p67 

    The roles of Mg2+ and Zn2+ ions in promoting phosphoryl transfer catalysed by alkaline phosphatase are yet to be fully characterised. We investigated the divalent metal ion requirements for the monoesterase and diesterase activities of calf intestinal alkaline phosphatase. The synergistic effect...

  • Protein Microchips in Quantitative Assays for Tumor Markers. Dementieva, E. I.; Rubina, A. Yu.; Darii, E. L.; Dyukova, V. I.; Zasedatelev, A. S.; Osipova, T. V.; Ryabykh, T. P.; Baryshnikov, A. Yu.; Mirzabekov, A. D. // Doklady Biochemistry & Biophysics;Mar/Apr2004, Vol. 395 Issue 1-6, p88 

    Presents a study which examined protein microchips in quantitative assays for tumor markers. Development of quantitative immunoassay for markers of occurring tumors; Role of tumor markers in diagnosing malignant tumors; Analysis of protein microchips for conducting an immunoassay for...

  • Commentary on: Implications of Thyroglobulin Antibody Positivity in Patients with Differentiated Thyroid Cancer: A Clinical Position Statement. Spencer, Carole // Thyroid;Oct2013, Vol. 23 Issue 10, p1190 

    The author reflects on interference of thyroglobulin autoantibody (TgAb) in Tg measurement, issues related to detection of TgAb and use of TgAb as a surrogate differentiated thyroid cancer (DTC) tumor marker. She reports that both competitive assays and noncompetitive assays such as immunometric...

  • Colorimetric Immunoassay for Detection of Tumor Markers. Yongmei Yin; Ya Cao; Yuanyuan Xu; Genxi Li // International Journal of Molecular Sciences;Dec2010, Vol. 11 Issue 12, p5077 

    Tumor markers are substances, usually proteins, produced by the body in response to cancer growth, or by the cancer tissue itself. They can be detected in blood, urine, or tissue samples, and the discovery and detection of tumor markers may provide earlier diagnosis of cancer and improved...

  • Usporedba dviju imunoanaliza za tumorske biljege CA19-9, CEA i AFP. Unic, Adiana; Derek, Lovorka; Stančin, Nevenka; Serdar, Tihana; Sprajc, Novka; Romic, Zeljko // Biochemia Medica;2010, Vol. 20 Issue 1, p82 

    Introduction: Monoclonal antibodies are used to detect serum antigens associated with specific malignancies. These tumor markers are most useful for monitoring response to therapy and detecting early relapse, but results obtained by different assays vary, and a change of method during follow-up...

  • Free light chains. Sheldon, Joanna // Annals of Clinical Biochemistry;Nov2007, Vol. 44 Issue 6, p503 

    The author reflects on the issues to be considered before replacing the tried, tested and reliable assays with the FREELITE assay. He said that the Bradwell study reported the development of FREELITE, a sensitive automated immunoassay for immunoglobulin free light chains. He emphasized that they...

  • Interpretation of Hormone Levels in Older Patients: Points for Consideration. Sztefko, Krystyna; Szybowska, Patrycja // International Journal of Endocrinology;2012, p1 

    Blood hormone and tumor marker concentrations are usually determined by immunochemical methods which are based on an unique reaction between antigen and assay capture antibody. Despite the speed and simplicity of assays performance on automatic immunochemistry platforms, the interpretation of...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics