Assessment of left ventricular compliance during heart preservation

Larson, D.F.; Gatewood, L.B.; Bowers, M.; Sethi, G.; Copeland, J.G.
January 1998
Perfusion;1998, Vol. 13 Issue 1, p67
Academic Journal
There is critical need for a greater number of donor hearts for transplantation. The demand can be relieved, in part, by an extension of preservation time. This necessitates new methods of preservation and development of means to assess the functional condition of the preserved heart. We report a heart-preservation system designed for long-term preservation support and discuss issues specifically related to extended heart preservation. This article presents methodology to assess ventricular compliance and to quantify coronary flow distribution during the use of microperfusion preservation. Ventricular adenosine triphosphate (ATP) concentrations are directly related to the immediate post-preservation function: however, direct measurement of ATP is not clinically available. Based on the premise that ventricular compliance relates directly to the ventricular ATP concentrations, we performed sequential ventricular compliance measurements using a simple left ventricular balloon during a 24 h preservation period. A porcine heart model was employed using a continuous, hypothermic, antegrade, microperfusion system for 24 h and measurements were made at specific intervals during the preservation time. The compliance measurements were ascertained by pressure--volume curves using a flaccid balloon inserted into the left ventricle through the mitral valve. In addition, to assess microvascular function during the preservation interval, regional coronary flow measurements were performed using a microsphere technique. We report that after 12 h of preservation there was a twofold reduction in ventricular compliance which decreased further by fivefold at 18 h. In contrast, there was a time-dependent decrease in left ventricular coronary flow, especially with the left-ventricular subendocardial region significantly decreasing by 50% at 12 h. In conclusion, a simple ventricular-compliance balloon provided a direct measurement of ventricular compliance of the preserved heart which may provide an indirect estimate of the ventricular high-energy phosphates of the preserved heart prior to transplantation.


Related Articles

  • Fructose 1,6-Diphosphate Administration Attenuates Post-Ischemic Ventricular Dysfunction Cohen, Jeffrey E.; Atluri, Pavan; Taylor, Matthew D.; Grand, Todd J.; Liao, George P.; Panlilio, Corinna M.; Suarez, Erik E.; Zentko, Suzanne E.; Hsu, Vivian M.; Berry, Mark F.; Smith, Maximillian J.; Gardner, Timothy J.; Sweeney, H. Lee; Woo, Y. Joseph // Heart, Lung & Circulation;Apr2006, Vol. 15 Issue 2, p119 

    Background: Cardiomyocyte energy production during ischemia depends upon anaerobic glycolysis inefficiently yielding two ATP per glucose. Substrate augmentation with fructose 1,6-diphosphate (FDP) bypasses the ATP consuming steps of glucokinase and phosphofructokinase thus yielding four ATP per...

  • Distinct ATP receptors on pain-sensing and stretch-sensing neurons. Cook, Sean P.; Vulchanova, Lucy // Nature;5/29/1997, Vol. 387 Issue 6632, p505 

    Reports on the development of a tissue culture system that allows the comparison of nociceptive and non-nociceptive rat sensory neurons. The effect of low concentrations of ATP; The support of the theory that P2X3 receptors mediate a form of nociception.

  • Membrane potassium currents in human radial artery and their regulation by nitric oxide donor. Zhang, Yongde; Tazzeo, Tracy; Chu, Victor; Janssen, Luke J. // Cardiovascular Research;Jul2006, Vol. 71 Issue 2, p383 

    Abstract: Objective: The human radial artery has demonstrated superior long-term results as a graft in coronary bypass surgery, but undesirable post-surgical spasm limits its clinical application. Few have examined its excitatory properties, especially the underlying ion channel mechanisms. In...

  • Is ATP (adenosine 5'-triphosphate), like STP, a performance-enhancing additive for the tanks of cancer patients? Jatoi, Aminah; Loprinzi, Charles L.; Jatoi, A; Loprinzi, C L; Sloan, J; Goldberg, R M // JNCI: Journal of the National Cancer Institute;02/16/2000, Vol. 92 Issue 4, p290 

    Editorial. Presents information on a study which aimed to determine the effect of adenosine 5'-triphosphate (ATP) infusions as therapy for cancer patients. Effect of ATP infusions on body weight change, muscle strength, serum albumin concentration, and quality-of-life scores.

  • Randomized clinical trial of adenosine 5'-triphosphate in patients with advanced non-small-cell lung cancer. Agteresch, Hendrik J.; Dagnelie, Pieter C.; Agteresch, H J; Dagnelie, P C; van der Gaast, A; Stijnen, T; Wilson, J H // JNCI: Journal of the National Cancer Institute;02/16/2000, Vol. 92 Issue 4, p321 

    Background: Extracellular adenosine 5'-triphosphate (ATP) is involved in the regulation of a variety of biologic processes, including neurotransmission, muscle contraction, and liver glucose metabolism, via purinergic receptors. In nonrandomized studies involving patients with...

  • Fuel the fire.  // Joe Weider's Muscle & Fitness;Feb92, Vol. 53 Issue 2, p120 

    Discusses the role of food as fuel to the chemical reaction of adenosine triphosphate (ATP) that triggers muscle contraction. Anaerobic pathway of muscle energetics; Producing energy from ATP; Protein's minor role; Energy formula; Rest as the essential ingredient in a vigorous life.

  • Regulation of rhythmic movements by purinergic neurotransmitters in frog embryos. Dale, Nicholas; Gilday, Deborah // Nature;9/19/1996, Vol. 383 Issue 6597, p259 

    Investigates whether the purinergic transmitters ATP and adenosine control run-down of swimming in the Xenopus embryo. The implications for episodic rhythmic motor behaviors in vertebrates; The use of specific agonists and antagonists for the purinergic receptors; The involvement of ATP and...

  • SPEED LIMITS. Brown, Guy C. // Sciences;Sep/Oct2000, Vol. 40 Issue 5, p32 

    Expounds on the physiology of an athlete's body. Linking of research advances in diet, training and motivation to enhanced athletic performance; Race within the body to supply the energy required by contracting muscles; Use of phosphocreatine reserves; Conversion of glycogen to lactic acid;...

  • The Welcome Resurgence of the α-Cell: A Pro Glucagon Commentary. Robertson, R. Paul // Diabetes;Nov2010, Vol. 59 Issue 11, p2735 

    The author explains the physiologic function of the islet α-cell. He cites the regulation of hepatic glycogenolysis as the primary function of α-cell, and explains the clinical relevance of such function. He then considers the idea that the SUR1 subunit of ATP-sensitive K+ (KATP)) is...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics