Heat stimulation reduces early adipogenesis in 3T3-L1 preadipocytes

Tomonobu Ezure; Satoshi Amano
June 2009
Endocrine (1355008X);Jun2009, Vol. 35 Issue 3, p402
Academic Journal
Abstract  In this study, we used 3T3-L1 preadipocytes as a model to investigate the effects of heat stimulation on adipogenesis, which is a key process in the development of obesity. Heat stimulation at 43°C for 60 min significantly reduced lipid accumulation, as measured by Oil Red-O assay. In the early phase of adipogenesis, heat stimulation almost completely blocked the increase of CCAAT/enhancer binding protein delta (C/EBPdelta) gene expression and delayed the onset of the increase of C/EBPbeta gene expression. The expression of proliferator-activated receptor gamma (PPARgamma), which is regulated by these factors, was also reduced. In the later phase of adipogenesis, the induction of adipocyte-specific genes, such as C/EBPalpha, adipocyte protein 2 (aP2), lipoprotein lipase (LPL), adiponectin, and glucose transporter 4 (Glut4), which are regulated by PPARgamma, was reduced. However, adipogenesis was not significantly reduced if heat stimulation was carried out after the early phase of adipogenesis. These results suggest that heat stimulation reduces adipogenesis by decreasing the expression of adipogenesis-related transcriptional factors during early adipogenesis.


Related Articles

  • Transcription Factor AP-2β, A Novel Candidate Gene for Metabolic Syndrome, Accelerates MCP-1 Gene Expression in 3T3-L1 Adipocytes. Kondo, Motoyuki; Maegawa, Hiroshi; Ikeda, Kazuhiro; Fuke, Tomoya; Ugi, Satoshi; Nishio, Yoshihiko; Maeda, Shiro; Kashiwagi, Atsunori // Diabetes;Jun2007 Supplement 1, Vol. 56, pA509 

    Transcription factor activating enhancer-binding protein-2β (AP-2β) is identified as a novel candidate gene for conferring susceptibility to obese type2 diabetes. We found that AP-2β is expressed in adipose tissue and its expression is increased according to the differentiation to...

  • Smart transcription factors. D'Arcangelo, Gabriella; Curran, Tom // Nature;7/27/1995, Vol. 376 Issue 6538, p292 

    Focuses on the article by Fred Petrij et al in the July 27, 1995 issue of `Nature' magazine establishing the link between cognitive function and the regulation of gene expression. Association between the Rubinstein-Tabi syndrome and mutations in the transcription co-activator known as...

  • Investigation of effects of three candidate genes on leg action and fat deposition traits in pigs. Makgahlela, M. L.; Fan, B.; Du, Z.-Q.; Rothschild, M. F. // South African Journal of Animal Science;2009, Vol. 39 Issue 2, p127 

    Data from 188 sows were used in the current study to examine the effects of high mobility group AT-hook1 (HMGA1), transcription factor 7-like-2 (TCF7L2) and insulin-like growth factor binding protein 3 (IGFBP3) genes on leg action and fat deposition traits, and further to explore the possible...

  • Peptidoglycan enhances transcriptional expression of CCAAT/enhancer-binding protein δ gene in mouse macrophages. Yu-Chiuan Huang; Wen-Chang Chang; Su, Jyan-Gwo J.; Jheng-Liang Cai; Chun-Chia Chen; Jan-Jong Hung; Yi-Wen Liu // Journal of Biomedical Science;May2007, Vol. 14 Issue 3, p407 

    Peptidoglycan-activated gene expression is mediated through various transcription factors including CCAAT/enhancer-binding protein δ (C/EBPδ). The purpose of the present study is to elucidate the mechanism of PGN-activated C/EBPδ gene. PGN stimulated C/EBPδ protein and mRNA...

  • Gene regulation: Wading in upstream. Flintoft, Louisa // Nature Reviews Genetics;Jan2005, Vol. 6 Issue 1, p7 

    Presents the results of a study on the evolution of gene expression in Saccharomyces cerevisiae. Factors behind the co-regulation of different groups of genes; Role of the transcription factor Rpn4 in the control of gene expression; Significance of an Rpn4 protein in binding specificities of...

  • Modular organization of the E2F1 activation domain and its interaction with general transcription factors TBP and TFIIH. Pearson, Angela; Greenblatt, Jack // Oncogene;11/27/97, Vol. 15 Issue 22, p2643 

    The transcriptional activator E2F1 regulates the expression of genes at the G1/S boundary. We have characterized interactions of the E2F1 activation domain with two general transcription factors, the TATA-box binding protein (TBP) and TFIIH. Two distinct binding sites on E2F1 were identified for...

  • Lateral Gene Expression in Drosophila Early Embryos Is Supported by Grainyhead-Mediated Activation and Tiers of Dorsally-Localized Repression. Garcia, Mayra; Stathopoulos, Angelike // PLoS ONE;2011, Vol. 6 Issue 12, p1 

    The general consensus in the field is that limiting amounts of the transcription factor Dorsal establish dorsal boundaries of genes expressed along the dorsal-ventral (DV) axis of early Drosophila embryos, while repressors establish ventral boundaries. Yet recent studies have provided evidence...

  • Towards biological characters of interactions between transcription factors and their DNA targets in mammals. Guangyong Zheng; Qi Liu; Guohui Ding; Chaochun Wei; Yixue Li // BMC Genomics;2012, Vol. 13 Issue 1, p388 

    Background: In post-genomic era, the study of transcriptional regulation is pivotal to decode genetic information. Transcription factors (TFs) are central proteins for transcriptional regulation, and interactions between TFs and their DNA targets (TFBSs) are important for downstream genes'...

  • Cyclic mechanical strain causes cAMP-response element binding protein activation by different pathways in cardiac fibroblasts. Husse, Britta; Isenberg, Gerrit // Heart International;Jan2010, Vol. 5 Issue 1, p10 

    The transcription factor cAMP-response element binding protein (CREB) mediates the mechanical strain-induced gene expression in the heart. This study investigated which signaling pathways are involved in the strain-induced CREB activation using cultured ventricular fibroblasts from adult rat...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics