TITLE

Tip-enhanced two-photon excited fluorescence microscopy with a silicon tip

AUTHOR(S)
Hayazawa, Norihiko; Furusawa, Kentaro; Taguchi, Atsushi; Kawata, Satoshi; Abe, Hiroshi
PUB. DATE
May 2009
SOURCE
Applied Physics Letters;5/11/2009, Vol. 94 Issue 19, p193112
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The use of silicon tips and nonlinear response of materials can be used in overcoming the two major drawbacks of the near-field fluorescence detection which are quenching and background signals. Silicon tips allow for quenching free detection while the two-photon excitation scheme selectively enhances the scattering rate near the tip apex, so that the fluorescence signal from this tiny volume outweighs the one from diffraction limited focused spot. We demonstrate tip-enhanced two-photon excited fluorescence microscopy using a commercially available silicon cantilever tip, and show that individual quantum dots can be clearly resolved with spatial separation of 70 nm.
ACCESSION #
39785818

 

Related Articles

  • Fluorescence interference-contrast microscopy of cell adhesion on oxidized silicon. Braun, D.; Fromherz, P. // Applied Physics A: Materials Science & Processing;1997, Vol. 65 Issue 4/5, p341 

    Standing modes of light in front of the reflecting surface of silicon modulate the excitation and emission of fluorescent dyes. This effect was used to determine the distance of a biomembrane from an oxidized silicon chip. The membrane of a red blood cell (ghost) was stained with a cyanine dye...

  • Fluorescence imaging in the last two decades. Miyawaki, Atsushi // Microscopy;Feb2013, Vol. 62 Issue 1, p63 

    In commemoration of the 20th anniversary of the molecular cloning of the gene for the green fluorescent protein from the jellyfish Aequorea victoria, I would like to reflect on the development of new fluorescence imaging technology in the last two decades. As this technology has become...

  • Seeing fluorescence at super-resolution. Evanko, Daniel // Nature Methods;Jan2008, Vol. 5 Issue 1, p22 

    The article reports on the transformation of fluorescence microscopy by methods to improve its resolving power. The methods for super-resolution fluorescence imaging are described, which only require a commonly available microscope, specialized but easily obtainable fluorescent labels,...

  • Dual-detection confocal fluorescence microscope needs no axial scanning. Wallace, John // Laser Focus World;Sep2013, Vol. 49 Issue 9, p21 

    The article focuses on a study conducted by researchers at the Korea Advanced Institute of Science and Technology and Hanyang University to create an instrument that only needs to be scanned in two dimensions for confocal fluorescence microscopy. It is noted that two pinhole-covered...

  • A pitfall in using BODIPY dyes to label lipid droplets for fluorescence microscopy. Ohsaki, Yuki; Shinohara, Yuki; Suzuki, Michitaka; Fujimoto, Toyoshi // Histochemistry & Cell Biology;Apr2010, Vol. 133 Issue 4, p477 

    The lipid droplet (LD) has become a focus of intense research. Fluorescence labeling is indispensable for the cell biological analysis of the LD, and a lipophilic fluorescence dye, BODIPY 493/503, which emits bright green fluorescence has been used extensively for LD labeling. The dye is...

  • Fluorescence interference-contrast microscopy on oxidized silicon using a monomolecular dye layer. Lambacher, Armin; Fromherz, Peter // Applied Physics A: Materials Science & Processing;1996, Vol. 63 Issue 3, p207 

    A silicon chip is covered by a monomolecular film of a fluorescence dye with silicon dioxide used as a spacer. The fluorescence depends on the distance of the dye from the silicon. The modulation of the intensity is described quantitatively by an optical theory which accounts for interference of...

  • Fluorescence microscopy: Strategic blinking. Lukinavičius, Gražvydas; Johnsson, Kai // Nature Chemistry;Aug2014, Vol. 6 Issue 8, p663 

    The article discusses the concept of single-molecule localization microscopy (SLM) which separated by switching between fluorescent on and non-fluorescent off states. It highlights the use of the hydroxymethyl silicon-rhodamine (HMSiR) in constructing the super-resolution images of microtubules....

  • Experimental Determination of the Fluorescence Quantum Yield of Semiconductor Nanocrystals. Laverdant, Julien; de Marcillac, Willy Daney; Barthou, Carlos; Vu Duc Chinh; Schwob, Catherine; Coolen, Laurent; Benalloul, Paul; Pham Thu Nga; Maître, Agnès // Materials (1996-1944);Jul2011, Vol. 4 Issue 7, p1182 

    Many studies have considered the luminescence of colloidal II-VI nanocrystals, both in solution at a collective scale and at an individual scale by confocal microscopy. The quantum yield is an important figure of merit for the optical quality of a fluorophore. We detail here a simple method to...

  • Characterization of blinking dynamics in quantum dot ensembles using image correlation spectroscopy. Bachir, Alexia I.; Durisic, Nela; Hebert, Benedict; Grütter, Peter; Wiseman, Paul W. // Journal of Applied Physics;3/15/2006, Vol. 99 Issue 6, p064503 

    Quantum dots (QDs) are being increasingly applied as luminescent labels in optical studies for biophysical and cell biological applications due to their unique spectroscopic properties. However, their fluorescence “blinking” characteristics that follow power law statistics make it...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics