Second harmonic generation χ tensor microscopy for tissue imaging

Wei-Liang Chen; Tsung-Hsian Li; Ping-Jung Su; Chen-Kuan Chou; Fwu, Peter Tramyeon; Sung-Jan Lin; Kim, Daekeun; So, Peter T. C.; Chen-Yuan Dong
May 2009
Applied Physics Letters;5/4/2009, Vol. 94 Issue 18, p183902
Academic Journal
Important biological molecules such as collagen and myosin are strong second harmonic generators. The polarization dependence of second-harmonic generation (SHG) is a property of the second order susceptibility elements (χ tensor) of the molecules that produce the signal. In this work, we used polarization SHG microscopy to image and map pixel scale polarization anisotropy information of collagen-muscle junction of chicken wing and the dermis of human skin. We show that the variation in the χ tensor components can be displayed as images to show the contrast between different molecular sources of SHG.


Related Articles

  • Imaging of ferroelectric domain walls by force microscopy. Saurenbach, F.; Terris, B. D. // Applied Physics Letters;4/23/1990, Vol. 56 Issue 17, p1703 

    We have imaged ferroelectric domain walls in the ferroelectric-ferroelastic material Gd2(Mo)3 using force microscopy. By using a mode of imaging developed for the detection of static surface charge, the force gradient due to the polarization charge at the sample surface was imaged. The signal...

  • Hot electron spin-valve effect in coupled magnetic layers. Celotta, R. J.; Unguris, J.; Pierce, D. T. // Journal of Applied Physics;5/15/1994, Vol. 75 Issue 10, p6452 

    Presents scanning electron microscopy with polarization analysis observations of magnetic exchange coupling in the Fe/Ag/Fe, Fe/Au/Fe and Fe/Cr/Fe systems. Experiment conducted; Discussion; Conclusion.

  • 180° surface domain wall magnetization profiles: Comparisons between scanning electron microscopy with polarization analysis measurements, magneto-optic Kerr microscopy measurements and micromagnetic models. Scheinfein, M. R.; Ryan, P. J.; Unguris, J.; Pierce, D. T.; Celotta, R. J. // Applied Physics Letters;10/22/1990, Vol. 57 Issue 17, p1817 

    We compare measurements of magnetization profiles across a 180° surface domain wall in a 0.24-μm-thick of Permalloy (Ni81Fe19), obtained with scanning electron microscopy with polarization analysis (SEMPA) and longitudinal magneto-optic (MO) Kerr microscopy with the predictions of a bulk...

  • Effect of depolarization of scattered evanescent waves on particle-trapped near-field scanning... Min Gu; Pu Chun Ke // Applied Physics Letters;7/12/1999, Vol. 75 Issue 2, p175 

    Reports that the degree of polarization of the scattered evanescent wave is measured with a laser-trapped particle for different incident angles. Improvement in the contrast of the evanescent wave interference pattern imaged in a particle-trapped near-field scanning optical microscope.

  • Research highlights.  // Nature Cell Biology;Jul2009, Vol. 11 Issue 7, p796 

    The article offers news briefs related to cell biology in 2009. A study has determined that polarized distribution of the kinase Pom1 functions as a cell size censor that controls mitotic through the Weel-inhibitory kinase cerebellar degeneration-related protein 2 (Cdr2). A research team has...

  • Band-selective 13C Homonuclear 3D Spectroscopy for Solid Proteins at High Field with Rotor-synchronized Soft Pulses. Zhou, Donghua H.; Kloepper, Kathryn D.; Winter, Kem A.; Rienstra, Chad M. // Journal of Biomolecular NMR;Apr2006, Vol. 34 Issue 4, p245 

    We demonstrate improved 3D 13C�13C�13C chemical shift correlation experiments for solid proteins, utilizing band-selective coherence transfer, scalar decoupling and homonuclear zero-quantum polarization transfer. Judicious use of selective pulses and a z-filter period suppress...

  • Variationally optimized basis orbitals for biological molecules. Ozaki, T.; Kino, H. // Journal of Chemical Physics;12/8/2004, Vol. 121 Issue 22, p10879 

    Numerical atomic basis orbitals are variationally optimized for biological molecules such as proteins, polysaccharides, and deoxyribonucleic acid within a density functional theory. Based on a statistical treatment of results of a fully variational optimization of basis orbitals ( full optimized...

  • Formation and observation of 50 nm polarized domains in PbZr[sub 1-x]Ti[sub x]O[sub 3] thin film.... Hidaka, T.; Maruyama, T. // Applied Physics Letters;4/22/1996, Vol. 68 Issue 17, p2358 

    Describes the formation and observation of 50 nanometer polarized domains in PbZr[sub 1-x]Ti[sub x]O[sub 3] thin film using an atomic force microscope. Relationship between the polarized domain and grain of the film; Application of pulse voltage to form the polarized domain; Observation of the...

  • Determining the spin polarization of surfaces by spin-polarized scanning tunneling spectroscopy. Kubetzka, A.; Pietzsch, O.; Bode, M.; Wiesendanger, R. // Applied Physics A: Materials Science & Processing;2003, Vol. 76 Issue 6, p873 

    During the last few years, spin-polarized scanning tunneling microscopy and spectroscopy has been developed as a reliable tool to image surface magnetic domain structures of bulk materials as well as thin films and nanostructured systems. In principle, this technique also allows for the...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics