Persantine Attenuates Hemorrhagic Shock-Induced P-Selectin Expression

Olinde, John G.; Zibari, Gazi B.; Brown M.D., Mark F.; Howell, James G.; Akgur, Feza M.; McDonald, John C.; Granger, D. Neil
December 2000
American Surgeon;Dec2000, Vol. 66 Issue 12, p1093
Academic Journal
Ischemia/reperfusion (I/R), a phenomenon that is associated with conditions such as organ transplantation, trauma, vascular disease, and stroke, involves the recruitment of activated and adherent leukocytes that subsequently mediate tissue injury. Endothelial cell adhesion molecules such as P-selectin mediate UR-induced leukocyte recruitment and allow the adherent leukocytes to damage the vascular wall and parenchymal cells. This study examines the influence of dypiridamole (persantine) on hemorrhagic shock (H/S)-induced P-selectin expression. H/S was induced in C57BL/6 mice by withdrawing blood to drop the mean arterial blood pressure to 30 to 35 mm Hg for 45 minutes. The mice were resuscitated by infusing the shed blood and Ringer's lactate (50% shed blood volume). In vivo P-selectin expression was determined using a dual monoclonal antibody technique in the heart, lung, liver, kidneys, stomach, small bowel, and colon of a control group, a hemorrhagic shock group, and a hemorrhagic shock group that was pretreated with Persantine (Boehringer, Ingelheim, Ingelheim, Germany). H/S significantly (P < 0.01) increased P-selectin expression in all regional vascular beds of untreated mice. Persantine treatment largely prevented the H/S-induced P-selectin expression in the same vascular beds. Persantine significantly attenuates the up-regulation of P-selectin in the hemorrhagic shock model.


Related Articles

  • Long-Term Effects of the Adenosine Kinase Inhibitor GP-515 on Hepatic Microcirculation Following Hemorrhagic Shock. Bulut, Kerem; Sargin, Bülent; Marzi, Ingo; Bauer, Clemens // European Journal of Trauma;Jun2003, Vol. 29 Issue 3, p139 

    Background: Microcirculatory perfusion failure, known as the "no-reflow phenomenon", often develops within a few days after ischemia-reperfusion injury and can be a severe problem following resuscitation. Investigations with the adenosine kinase inhibitor GP-515 have shown beneficial protective...

  • Reversal of Hemorrhagic Shock-Associated Hepatic Ischemia-Reperfusion Injury With N-acetylcysteine. Allan, Patrick F.; Bloom, Brian B.; Wanek, Sandra // Military Medicine;Mar2011, Vol. 176 Issue 3, p332 

    Background: The underpinning pathophysiology, prevalence, and clinical relevance particular to hemorrhagic shock-induced liver damage have been explored only recently. Importantly, several investigators have revealed that an aberrant periportal endothelial response, characterized by the early...

  • Compared effects of inhibition and exogenous administration of hydrogen sulphide in ischaemia-reperfusion injury. Issa, Khodor; Kimmoun, Antoine; Collin, Solène; Ganster, Frederique; Fremont-Orlowski, Sophie; Asfar, Pierre; Mertes, Paul-Michel; Levy, Bruno // Critical Care;2013, Vol. 17 Issue 4, p1 

    Introduction: Haemorrhagic shock is associated with an inflammatory response consecutive to ischaemia-reperfusion (I/R) that leads to cardiovascular failure and organ injury. The role of and the timing of administration of hydrogen sulphide (H2S) remain uncertain. Vascular effects of H2S are...

  • Effect of N-acetylcysteine in hearts of rats submitted to controlled hemorrhagic shock. de Oliveira Filho, Luiz Dantas; Saad, Karen Ruggeri; Saad, Paulo Fernandes; Koike, Marcia Kiyomi; da Silva, Sônia Maria; de Souza Montero, Edna Frasson // Brazilian Journal of Cardiovascular Surgery;Mar/Apr2015, Vol. 30 Issue 2, p173 

    Introduction: Pharmacological therapy is a strategy for the prevention of complications associated with ischemia and reperfusion injury that occurs after volume replacement in the treatment of hemorrhagic shock. Objective: The aim of this study was to evaluate the effect of N-acetylcysteine...

  • Cytokines and the immunomodulatory function of the vagus nerve. G. R. Johnston; N. R. Webster // BJA: The British Journal of Anaesthesia;Apr2009, Vol. 102 Issue 4, p453 

    Cytokine synthesis and release is an essential component of the innate immune system, but inappropriate, excessive production results in a generalized systemic inflammatory response which damages distant organs. Recent research has identified an immunomodulatory function of the vagus nerve...

  • Volaemic resuscitation in critical patients with severe haemorrhagic shock. Complications due to impaired microvascular system and ischaemia/reperfusion syndrome: A Case Report. BEDREAG, OVIDIU HOREA; ROSU, OANA MARIA; ROGOBETE, ALEXANDRU FLORIN; SARANDAN, MIRELA; CRADIGATI, ALINA CARMEN; PAPURICA, MARIUS; TUNESCU, BOGDAN; POPESCU, MIRCEA; DRAGUSANU, SILVIU; COJOCARU, TUDOR; SANDESC, DOREL // Signa Vitae;Dec2015, Vol. 10 Issue 2, p218 

    Severe trauma is one of the most common causes of death all around the world. Complications of haemorrhagic shock, such as coagulopathy, hypothermia and metabolic acidosis, form the lethal triad in trauma. In this case report we present a male patient admitted with severe haemorrhagic shock -...

  • Blast injury and its effects on the endothelium. Spear, A.; Davies, E.; Kirkman, E.; Taylor, C.; Midwinter, M.; Watts, S. // Proceedings of the Physiological Society;2013, p789P 

    Introduction: It is known that the tolerance for return to normal neuromuscular function in tissue exposed to ischemia with concomitant hemorrhagic shock is significantly reduced compared to ischemia alone (Hancock et al., 2011). With an increased incidence of explosive related injuries in...

  • Acute limb ischaemia. Callum, Ken; Bradbury, Andrew // BMJ: British Medical Journal (International Edition);03/18/2000, Vol. 320 Issue 7237, p764 

    Provides information on the classification of acute limb ischaemia. Clinical features and aetiology of acute limb ischaemia; General measures for acute limb ischaemia; Details on reperfusion syndrome.

  • CORRIGENDUM: Novel n-3 Immunoresolvents: Structures and Actions. Dalli, Jesmond; Colas, Romain A.; Serhan, Charles N. // Scientific Reports;10/24/2014, p1 

    A correction to the article "Novel n-3 Immunoresolvents: Structures and Actions" by Jesmond Dalli, Romain A. Colas and Charles N. Serhan is presented.


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics