Osteoblast Differentiation Stage-Specific Expression of the Pyrophosphate-Generating Enzyme PC-1

Hatch, Nan E.; Franceschi, Renny T.
December 2008
Cells Tissues Organs;2008, Vol. 189 Issue 1-4, p65
Academic Journal
Fibroblast growth factor (FGF) signaling plays a critical role in skeletal development, yet the mechanism by which FGFs affect bone mineralization is not well understood. Review of the literature investigating effects of FGF signaling on bone mineralization indicates that FGFs may stimulate expression of factors that prevent mineralization in the short term and enhance mineralization in the long term. Pyrophosphate is an ideal example of a factor that, dependent upon environment, has the capacity to inhibit or enhance mineralization. PC-1 is the primary generator of pyrophosphate in osteoblastic cells; therefore, regulated expression of PC-1 by FGFs may be a principal mechanism by which FGF signaling affects bone mineralization. We previously showed that FGF2 induces PC-1 expression in preosteoblastic cells and that this induction is differentiation stage dependent. In order to more directly investigate the mechanism by which PC-1 expression is regulated, we have cloned a 2.8-kb region of the PC-1 gene promoter and constructed a PC-1 gene promoter/firefly luciferase reporter construct. Results indicate that this construct is specifically responsive to FGF2 or ascorbate (an inducer of osteoblast differentiation). Promoter responsiveness to FGF2 is significantly diminished upon osteoblast differentiation, and increases in promoter activity that occur with osteoblast differentiation are inhibited by FGF2 treatment. These results indicate that the mechanism of PC-1 induction by FGF2 in preosteoblastic cells is distinct from the mechanism of induction that occurs with osteoblast differentiation. These results also indicate that PC-1 may play multiple and distinct roles in the development of mineralized tissues. Copyright © 2008 S. Karger AG, Basel


Related Articles

  • Fibroblast growth factor 2 and forskolin induce mineralization-associated genes in two kinds of osteoblast-like cells. Nakayama, Yohei; Yang, Li; Takai, Hideki; Kaneko, Hirotoshi; Abiko, Yoshimitsu; Ogata, Yorimasa // Journal of Oral Science;Sep2012, Vol. 54 Issue 3, p251 

    Fibroblast growth factor 2 (FGF2) and cyclic AMP (cAlVlP) play critical roles in controlling the differentiation of osteoblasts and mineralization of bone. We have previously reported that each of FGF2 and forskolin (FSK) alone increase transcription of the bone sialoprotein (BSP) gene, and that...

  • FGF2-induced chromatin remodeling regulates CNTF-mediated gene expression and astrocyte differentiation. Mi-Ryoung Song, Christopher B.; Ghosh, Anirvan // Nature Neuroscience;Mar2004, Vol. 7 Issue 3, p229 

    The generation of distinct cell types during development depends on the competence of progenitor populations to differentiate along specific lineages. Here we investigate the mechanisms that regulate competence of rodent cortical progenitors to differentiate into astrocytes in response to...

  • Dynamic expression of a Hydra FGF at boundaries and termini. Lange, Ellen; Bertrand, Stephanie; Holz, Oliver; Rebscher, Nicole; Hassel, Monika // Development Genes & Evolution;Dec2014, Vol. 224 Issue 4-6, p235 

    Guidance of cells and tissue sheets is an essential function in developing and differentiating animal tissues. In Hydra, where cells and tissue move dynamically due to constant cell proliferation towards the termini or into lateral, vegetative buds, factors essential for guidance are still...

  • The Significance of Fibroblast Growth Factor Receptor 2 Expression in Differentiation of Hepatocellular Carcinoma. Harimoto, Norifumi; Taguchi, Kenichi; Shirabe, Ken; Adachi, Eisuke; Sakaguchi, Yoshihisa; Toh, Yasushi; Okamura, Takeshi; Kayashima, Hiroto; Taketomi, Akinobu; Maehara, Yoshihiko // Oncology;2010, Vol. 78 Issue 5/6, p361 

    Fibroblast growth factor receptors (FGFRs) have been reported to be involved in the progression of many cancers. The aim of this study is to clarify the significance of FGFR2 expression in the differentiation of hepatocellular carcinoma (HCC). One nodule-in-nodule HCC sample was obtained from a...

  • Expression of a Functional Recombinant Human Basic Fibroblast Growth Factor from Transgenic Rice Seeds. Na An; Jiquan Ou; Daiming Jiang; Liping Zhang; Jingru Liu; Kai Fu; Ying Dai; Daichang Yang // International Journal of Molecular Sciences;Feb2013, Vol. 14 Issue 2, p3556 

    Basic fibroblast growth factor (FGF-2) is an important member of the FGF gene family. It is widely used in clinical applications for scald and wound healing in order to stimulate cell proliferation. Further it is applied for inhibiting stem cell differentiation in cultures. Due to a shortage of...

  • Fgf3 and Fgf10a Work in Concert to Promote Maturation of the Epibranchial Placodes in Zebrafish. McCarroll, Matthew N.; Nechiporuk, Alex V. // PLoS ONE;Dec2013, Vol. 8 Issue 12, p1 

    Essential cellular components of the paired sensory organs of the vertebrate head are derived from transient thickenings of embryonic ectoderm known as cranial placodes. The epibranchial (EB) placodes give rise to sensory neurons of the EB ganglia that are responsible for relaying visceral...

  • Fibroblast Growth Factor Receptor 2 (FGFR2) Is Required for Corneal Epithelial Cell Proliferation and Differentiation during Embryonic Development. Zhang, Jinglin; Upadhya, Dinesh; Lu, Lin; Reneker, Lixing W. // PLoS ONE;Jan2015, Vol. 10 Issue 1, p1 

    Fibroblast growth factors (FGFs) play important roles in many aspects of embryonic development. During eye development, the lens and corneal epithelium are derived from the same surface ectodermal tissue. FGF receptor (FGFR)-signaling is essential for lens cell differentiation and survival, but...

  • A Signaling Pathway at the Heart of Muscle Development. Robinson, Richard // PLoS Biology;Oct2005, Vol. 3 Issue 10, p1 

    This article focuses on a new clinical study according to which researcher K. Vijay Raghavan and colleagues have identified several key molecules involved in adult founder cell formation. In the fly embryo, founder cells differentiate from myoblasts through the actions of a membrane-bound...

  • Fgf8 is required for outgrowth and patterning of the limbs. Moon, Anne M.; Capecchi, Mario R. // Nature Genetics;Dec2000, Vol. 26 Issue 4, p455 

    The expression pattern and activity of fibroblast growth factor-8 (FGF8) in experimental assays indicate that it has important roles in limb development[SUP1-3], but early embryonic lethality resulting from mutation ofFgf8 in the germ line of mice has prevented direct assessment of these...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics