TITLE

Current applications and future trends of molecular diagnostics in clinical bacteriology

AUTHOR(S)
Weile, Jan; Knabbe, Cornelius
PUB. DATE
June 2009
SOURCE
Analytical & Bioanalytical Chemistry;Jun2009, Vol. 394 Issue 3, p731
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Molecular diagnostics of infectious diseases, in particular, nucleic-acid-based methods, are the fastest growing field in clinical laboratory diagnostics. These applications are stepwise replacing or complementing culture-based, biochemical, and immunological assays in microbiology laboratories. The first-generation nucleic acid assays were monoparametric such as conventional tests, determining only a single parameter. Improvements and new approaches in technology now open the possibility for the development of multiparameter assays using microarrays, multiplex nucleic acid amplification techniques, or mass spectrometry, while the introduction of closed-tube systems has resulted in rapid microbial diagnostics with a subsequently reduced contamination risk. Whereas the first assays were focused on the detection and identification of microbial pathogens, these new technologies paved the way for the parallel determination of multiple antibiotic resistance determinants or to perform microbial epidemiology and surveillance on a genetic level.
ACCESSION #
38798704

 

Related Articles

  • Proteomics: Getting the numbers right. Cesari, Francesca // Nature Reviews Molecular Cell Biology;Sep2009, Vol. 10 Issue 9, p577 

    The article reports on the study that develops an integrated mass spectrometry (MS) strategy to estimate the cellular concentration of proteins for a large fraction of the proteome of the bacterial human pathogen Leptospira interrogans. It suggests that the results confirmed the accuracy of the...

  • Rapid identification of microorganisms by mass spectrometry: improved performance by incorporation of in-house spectral data into a commercial database. Sogawa, Kazuyuki; Watanabe, Masaharu; Sato, Kenichi; Segawa, Syunsuke; Miyabe, Akiko; Murata, Syota; Saito, Tomoko; Nomura, Fumio // Analytical & Bioanalytical Chemistry;Oct2012, Vol. 403 Issue 7, p1811 

    Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is increasingly used as a microbial diagnostic method for species identification of pathogens. However, MALDI-TOF identification of bacteria at the species level remains unsatisfactory, with the major...

  • MALDI-TOF Mass Spectrometry Detection of Pathogens in Vectors: The Borrelia crocidurae/Ornithodoros sonrai Paradigm. Fotso Fotso, Aurélien; Mediannikov, Oleg; Diatta, Georges; Almeras, Lionel; Flaudrops, Christophe; Parola, Philippe; Drancourt, Michel // PLoS Neglected Tropical Diseases;Jul2014, Vol. 8 Issue 7, p1 

    Background: In Africa, relapsing fever borreliae are neglected vector-borne pathogens that cause mild to deadly septicemia and miscarriage. Screening vectors for the presence of borreliae currently requires technically demanding, time- and resource-consuming molecular methods. Matrix-assisted...

  • Detection and quantitation of normal and variant haemoglobins: an analytical review. Wild, Barbara J; Bain, Barbara J // Annals of Clinical Biochemistry;Sep2004, Vol. 41 Issue 5, p355 

    As well as six 'normal' haemoglobins that occur at various stages of development, more than 800 abnormal or variant haemoglobins have been described. Many of these variant haemoglobins have no significant clinical consequences apart from causing confusion to clinicians and in laboratories;...

  • Mycobacterium avium subsp. paratuberculosis as a template in the evaluation of automated kits for DNA extraction from bovine organs. Pozzato, Nicola; Stefani, Elisabetta; Capello, Katia; Muliari, Riccardo; Vicenzoni, Gaddo // World Journal of Microbiology & Biotechnology;Jan2011, Vol. 27 Issue 1, p31 

    Molecular diagnostic tests are widely implemented in animal and human health microbiology. Efficient nucleic acid extraction methods are essential in diagnostic laboratories and automation is a valuable tool for those with high throughput activity. Nucleic acid extraction protocols present...

  • Rapid identification of Burkholderia mallei and Burkholderia pseudomallei by intact cell Matrix-assisted Laser Desorption/Ionisation mass spectrometric typing. Karger, Axel; Stock, Rdiger; Ziller, Mario; Elschner, Mandy C.; Bettin, Barbara; Melzer, Falk; Maier, Thomas; Kostrzewa, Markus; Scholz, Holger C.; Neubauer, Heinrich; Tomaso, Herbert // BMC Microbiology;2012, Vol. 12 Issue 1, p229 

    Background: Burkholderia (B.) pseudomallei and B. mallei are genetically closely related species. B. pseudomallei causes melioidosis in humans and animals, whereas B. mallei is the causative agent of glanders in equines and rarely also in humans. Both agents have been classified by the CDC as...

  • DETECTION: IT'S NOT JUST DNA. F.H.B, Faith Hickman // Odyssey;Mar2004, Vol. 13 Issue 3, p33 

    Presents information on various methods of detecting pathogens like, antibody-based methods, mass spectrometry, and others.

  • DNA interactions and photocatalytic strand cleavage by artificial nucleases based on water-soluble gold(III) porphyrins. Haeubl, Martin; Reith, Lorenz Michael; Gruber, Bernadette; Karner, Uwe; M�ller, Norbert; Kn�r, G�nther; Schoefberger, Wolfgang // Journal of Biological Inorganic Chemistry;Sep2009, Vol. 14 Issue 7, p1037 

    The novel gold porphyrin complex (5,10,15-tris( N-methylpyridinium-4-yl)-20-(1-pyrenyl)-porphyrinato)gold(III) chloride, [AuIII(TMPy3Pyr1P)](Cl)4, was prepared and characterized by optical spectroscopy, high-resolution nuclear magnetic resonance (NMR), and electrospray mass spectrometry. This...

  • A Better Route for Diagnostics? Perkel, Jeffrey M. // Drug Discovery & Development;Jun2012, Vol. 15 Issue 6, p8 

    The article reports on the Human Genome Project that yields substantial benefits in diagnostics. It mentions researchers can query biomolecules with the rapid advancement in DNA sequencing, mass spectrometry, and microarrays. Further it mentions omics diagnostics development involves...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics