A system for high-resolution depth-resolved optical imaging of fluorescence and absorption contrast

Yuan, Baohong; Burgess, Sean A.; Iranmahboob, Amir; Bouchard, Matthew B.; Lehrer, Nicole; Bordier, Clémence; Hillman, Elizabeth M. C.
April 2009
Review of Scientific Instruments;Apr2009, Vol. 80 Issue 4, p043706
Academic Journal
Laminar optical tomography (LOT) is a new three-dimensional in vivo functional optical imaging technique. Adopting a microscopy-based setup and diffuse optical tomography (DOT) imaging principles, LOT can perform both absorption- and fluorescence-contrast imaging with higher resolution (100–200 μm) than DOT and deeper penetration (2–3 mm) than laser scanning microscopy. These features, as well as a large field of view and acquisition speeds up to 100 frames per second, make LOT suitable for depth-resolved imaging of stratified tissues such as retina, skin, endothelial tissues and the cortex of the brain. In this paper, we provide a detailed description of a new LOT system design capable of imaging both absorption and fluorescence contrast, and present characterization of its performance using phantom studies.


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