TITLE

Genome sequencing and analysis reveals possible determinants of Staphylococcus aureus nasal carriage

AUTHOR(S)
Sivaraman, Karthikeyan; Venkataraman, Nitya; Tsai, Jennifer; Dewell, Scott; Cole, Alexander M.
PUB. DATE
January 2008
SOURCE
BMC Genomics;2008, Vol. 9, Special section p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: Nasal carriage of Staphylococcus aureus is a major risk factor in clinical and community settings due to the range of etiologies caused by the organism. We have identified unique immunological and ultrastructural properties associated with nasal carriage isolates denoting a role for bacterial factors in nasal carriage. However, despite extensive molecular level characterizations by several groups suggesting factors necessary for colonization on nasal epithelium, genetic determinants of nasal carriage are unknown. Herein, we have set a genomic foundation for unraveling the bacterial determinants of nasal carriage in S. aureus. Results: MLST analysis revealed no lineage specific differences between carrier and non-carrier strains suggesting a role for mobile genetic elements. We completely sequenced a model carrier isolate (D30) and a model non-carrier strain (930918-3) to identify differential gene content. Comparison revealed the presence of 84 genes unique to the carrier strain and strongly suggests a role for Type VII secretion systems in nasal carriage. These genes, along with a putative pathogenicity island (SaPIBov) present uniquely in the carrier strains are likely important in affecting carriage. Further, PCR-based genotyping of other clinical isolates for a specific subset of these 84 genes raise the possibility of nasal carriage being caused by multiple gene sets. Conclusion: Our data suggest that carriage is likely a heterogeneic phenotypic trait and implies a role for nucleotide level polymorphism in carriage. Complete genome level analyses of multiple carriage strains of S. aureus will be important in clarifying molecular determinants of S. aureus nasal carriage.
ACCESSION #
38123032

 

Related Articles

  • Polymorphisms in the 5'-untranslated region of the human serotonin receptor 1B (HTR1B) gene affect gene expression. Duan, J.; Sanders, A. R.; Moien, J. E.Vander; Martinolich, L.; Mowry, B. J.; Levinson, D. F.; Crowe, R. R.; Silverman, J. M.; Gejman, P. V. // Molecular Psychiatry;2003, Vol. 8 Issue 11, p901 

    We present evidence of complex balancing regulation of HTR1B transcription by common polymorphisms in its promoter. Computational analysis of the HTR1B gene predicted that a 5' segment, spanning common DNA sequence variations, T-261G, A-161T, and -182INS/DEL-181, contained a putative functional...

  • Whole Genome Analysis of a Community-Associated Methicillin-Resistant Staphylococcus aureus ST59 Isolate from a Case of Human Sepsis and Severe Pneumonia in China. Qu, Tingting; Feng, Ye; Jiang, Yan; Zhu, Peiqiong; Wei, Zeqing; Chen, Yan; Otto, Michael; Yu, Yunsong // PLoS ONE;Feb2014, Vol. 9 Issue 2, p1 

    We report a case of necrotizing pneumonia in a young patient caused by community acquired-methicillin resistant Staphylococcus aureus (CA-MRSA) in a teaching hospital in the People’s Republic of China. The patient had a typical clinical presentation and was successfully treated with...

  • The ORFeome of Staphylococcus aureus v 1.1. Brandner, Christina J.; Maier, Richard H.; Henderson, Daryl S.; Hintner, Helmut; Bauer, Johann W.; Önder, Kamil // BMC Genomics;2008, Vol. 9, Special section p1 

    Background: The bacterium Staphylococcus aureus causes significant morbidity and mortality in humans, primarily due to the emergence of strains that are resistant to antibiotics -- notably methicillin-resistant S. aureus (MRSA) isolates. Development of effective strategies for the control and...

  • A Novel Computational Method Identifies Intra- and Inter-Species Recombination Events in Staphylococcus aureus and Streptococcus pneumoniae. Sanguinetti, Lisa; Toti, Simona; Reguzzi, Valerio; Bagnoli, Fabio; Donati, Claudio // PLoS Computational Biology;Sep2012, Vol. 8 Issue 9, p1 

    Advances in high-throughput DNA sequencing technologies have determined an explosion in the number of sequenced bacterial genomes. Comparative sequence analysis frequently reveals evidences of homologous recombination occurring with different mechanisms and rates in different species, but the...

  • Use of pyrosequencing to identify point mutations in domain V of 23S rRNA genes of linezolid-resistant Staphylococcus aureus and Staphylococcus epidermidis. Zhu, W.; Tenover, F. C.; Limor, J.; Lonsway, D.; Prince, D.; Dunne, Jr., W. M.; Patel, J. B. // European Journal of Clinical Microbiology & Infectious Diseases;Mar2007, Vol. 26 Issue 3, p161 

    A pyrosequencing assay was used for the rapid characterization of linezolid-resistant isolates of Staphylococcus aureus and Staphylococcus epidermidis. The assay identified base substitutions in copies of the 23S rRNA gene and determined the percentage of alleles with the mutation. Modifications...

  • Genetic Variation in the Staphylococcus aureus 8325 Strain Lineage Revealed by Whole-Genome Sequencing. Bæk, Kristoffer T.; Frees, Dorte; Renzoni, Adriana; Barras, Christine; Rodriguez, Natalia; Manzano, Caroline; Kelley, William L. // PLoS ONE;Sep2013, Vol. 8 Issue 9, p1 

    Staphylococcus aureus strains of the 8325 lineage, especially 8325-4 and derivatives lacking prophage, have been used extensively for decades of research. We report herein the results of our deep sequence analysis of strain 8325-4. Assignment of sequence variants compared with the reference...

  • Polymorphism, genetic exchange and intragenic recombination of the aureolysin gene among Staphylococcus aureus strains. Sabat, Artur J.; Wladyka, Benedykt; Kosowska-Shick, Klaudia; Grundmann, Hajo; Van Dijl, Jan Maarten; Kowal, Julia; Appelbaum, Peter C.; Dubin, Adam; Hryniewicz, Waleria // BMC Microbiology;2008, Vol. 8, Special section p1 

    Background: Staphylococcus aureus expresses several proteases, which are thought to contribute to the virulence of this bacterium. Here we focus on aureolysin, the major thermolysin-like metalloprotease. Despite the importance of aureolysin in the physiology and pathogenesis of S. aureus,...

  • Toward an Understanding of the Evolution of Staphylococcus aureus Strain USA300 during Colonization in Community Households. Uhlemann, Anne-Catrin; Kennedy, Adam D.; Martens, Craig; Porcella, Stephen F.; DeLeo, Frank R.; Lowy, Franklin D. // Genome Biology & Evolution;Sep2012, Vol. 4 Issue 9, p1275 

    Staphylococcus aureus is a frequent cause of serious infections and also a human commensal. The emergence of community-associated methicillin-resistant S. aureus led to a dramatic increase in skin and soft tissue infections worldwide. This epidemic has been driven by a limited number of clones,...

  • CA repeat polymorphism in the promoter region of the COL1A2 gene. Dietzsch, Erin; Parker, M. Iqbal // Journal of Human Genetics;1999, Vol. 44 Issue 6, p419 

    Abstract A polymorphic CA dinucleotide repeat sequence has been identified within the promoter of the human alpha 2(I) procollagen gene, located at 7q21.3-q22.1. Nine alleles have been identified in unrelated individuals and the observed heterozygosity for the polymorphism was 0.66. This marker...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics