Diversity in genomic organisation, developmental regulation and distribution of the murine PR72/B″ subunits of protein phosphatase 2A

Zwaenepoe, Karen; Louis, Justin V.; Goris, Jozef; Janssens, Veerle
January 2008
BMC Genomics;2008, Vol. 9, Special section p1
Academic Journal
Background: Protein phosphatase 2A (PP2A) is a serine/threonine-specific phosphatase displaying vital functions in growth and development through its role in various signalling pathways. PP2A holoenzymes comprise a core dimer composed of a catalytic C and a structural A subunit, which can associate with a variable B-type subunit. The importance of the B-type subunits for PP2A regulation cannot be overestimated as they determine holoenzyme localisation, activity and substrate specificity. Three B-type subunit families have been identified: PR55/B, PR61/B' and PR72/ B", of which the latter is currently the least characterised. Results: We deduced the sequences and genomic organisation of the different murine PR72/B" isoforms: three genes encode nine isoforms, five of which are abundantly expressed and give rise to genuine PP2A subunits. Thereby, one novel subunit was identified. Using Northern blotting, we examined the tissue-specific and developmental expression of these subunits. All subunits are highly expressed in heart, suggesting an important cardiac function. Immunohistochemical analysis revealed a striated expression pattern of PR72 and PR130 in heart and skeletal muscle, but not in bladder smooth muscle. The subcellular localisation and cell cycle regulatory ability of several PR72/ B" isoforms were determined, demonstrating differences as well as similarities. Conclusion: In contrast to PR55/B and PR61/B', the PR72/B" family seems evolutionary more divergent, as only two of the murine genes have a human orthologue. We have integrated these results in a more consistent nomenclature of both human and murine PR72/B" genes and their transcripts/proteins. Our results provide a platform for the future generation of PR72/B" knockout mice.


Related Articles

  • Pathogenesis of hypertrophic cardiomyopathy caused by myozenin 2 mutations is independent of calcineurin activity. Ruggiero, Alessandra; Chen, Suet Nee; Lombardi, Raffaella; Rodriguez, Gabriela; Marian, Ali J. // Cardiovascular Research;Jan2013, Vol. 97 Issue 1, p44 

    Aims The role of calcineurin protein phosphatase 2B (PP2B) in the pathogenesis of human hypertrophic cardiomyopathy (HCM) remains unsettled. We determined potential involvement of calcineurin in the pathogenesis of HCM caused by mutations in myozenin 2 (MYOZ2), an inhibitor of calcineurin....

  • Transcriptome sequencing and microarray design for functional genomics in the extremophile Arabidopsis relative Thellungiella salsuginea (Eutrema salsugineum). Yang Ping Lee; Giorgi, Federico M.; Lohse, Marc; Kvederaviciute, Kotryna; Klages, Sven; Usadel, Björn; Meskiene, Irute; Reinhardt, Richard; Hincha, Dirk K. // BMC Genomics;2013, Vol. 14 Issue 1, p1 

    Background Most molecular studies of plant stress tolerance have been performed with Arabidopsis thaliana, although it is not particularly stress tolerant and may lack protective mechanisms required to survive extreme environmental conditions. Thellungiella salsuginea has attracted interest as...

  • Protein phosphatase 2A contributes to the cardiac dysfunction induced by endotoxemia. Melanie Marshall; Narayana Anilkumar; Joanne Layland; Simon J. Walker; Jonathan C. Kentish; Ajay M. Shah; Alison C. Cave // Cardiovascular Research;Apr2009, Vol. 82 Issue 1, p67 

    Aims Sepsis-associated cardiac dysfunction represents an intrinsic impairment of cardiomyocyte function due in part to a decrease in myofilament Ca2+ sensitivity associated with a sustained increase in cardiac troponin I (cTnI) phosphorylation at Ser23/24. Dephosphorylation of cTnI is under...

  • A comprehensive analysis of protein phosphatases in rice and Arabidopsis. Meng Yang; Shuhui Song; Guiming Liu; Kaifu Chen; Xiangjun Tian; Zhizhuang Joe Zhao; Songnian Hu; Jun Yu // Plant Systematics & Evolution;Nov2010, Vol. 289 Issue 3/4, p111 

    Protein phosphatases play essential roles in many cellular processes through the reversible protein phosphorylation that dictates many signal transduction pathways among organisms. Based on an in silico analysis, we classified 163 and 164 non-redundant protein phosphatases in rice and...

  • A comprehensive platform for highly multiplexed mammalian functional genetic screens. Ketela, Troy; Heisler, Lawrence E.; Brown, Kevin R.; Ammar, Ron; Kasimer, Dahlia; Surendra, Anuradha; Ericson, Elke; Blakely, Kim; Karamboulas, Dina; Smith, Andrew M.; Durbic, Tanja; Arnoldo, Anthony; Kahlin Cheung-Ong; Koh, Judice L. Y.; Gopal, Shuba; Cowley, Glenn S.; Xiaoping Yang; Grenier, Jennifer K.; Giaever, Guri; Root, David E. // BMC Genomics;2011, Vol. 12 Issue 1, p213 

    Background: Genome-wide screening in human and mouse cells using RNA interference and open reading frame over-expression libraries is rapidly becoming a viable experimental approach for many research labs. There are a variety of gene expression modulation libraries commercially available,...

  • Annotation of novel transcripts putatively relevant for bovine fat metabolism. Eberlein, Annett; Kalbe, Claudia; Goldammer, Tom; Brunner, Ronald M.; Kuehn, Christa; Weikard, Rosemarie // Molecular Biology Reports;Jun2011, Vol. 38 Issue 5, p2975 

    Two bovine transcripts encoded by the interleukin-1 receptor-associated kinase 1 ( IRAK1) gene and the locus LOC618944 predicted as similar to human chromosome 6 open reading frame 52 ( C6orf52) gene had indicated divergent expression in bovine skeletal muscle containing different amount of...

  • The CAG repeat in SCA12 functions as a cis element to up-regulate PPP2R2B expression. Chih-Hsin Lin; Chiung-Mei Chen; Yi-Ting Hou; Yih-Ru Wu; Hsiu-Mei Hsieh-Li; Ming-Tsan Su; Guey-Jen Lee-Chen // Human Genetics;Aug2010, Vol. 128 Issue 2, p205 

    PPP2R2B, a protein widely expressed in neurons, regulates the protein phosphatase 2A (PP2A) activity for dephosphorylation of tau and other substrates. CAG repeat expansion at the 5′-end of the PPP2R2B gene causes autosomal dominant spinocerebellar ataxia type 12. In the present study, we...

  • Molecular Cloning of the Genes Encoding the PR55/Bβ/δ Regulatory Subunits for PP-2A and Analysis of Their Functions in Regulating Development of Goldfish, Carassius auratus. Jun-Qiong Zhao; Si-Si Xie; Wen-Bin Liu; Ya-Mei Xiao; Xiao-Ming Zeng; Mi Deng; Lili Gong; Jin-Ping Liu; Pei-Chao Chen; Jie Zhou; Xiao-Hui Hu; Jia-Han Lv; Xiang-Qian Yu; Dao Wang; Chi Li; Yun-Lei Peng; Gao-Peng Liao; Yun Liu; Wan-Cheng Li, David // Gene Regulation & Systems Biology;2010, Issue 4, p135 

    The protein phosphatase-2A (PP-2A), one of the major phosphatases in eukaryotes, is a heterotrimer, consisting of a scaffold A subunit, a catalytic C subunit and a regulatory B subunit. Previous studies have shown that besides regulating specific PP-2A activity, various B subunits encoded by...

  • Serine / threonine protein phosphatase 5 (PP5) participates in the regulation of glucocorticoid receptor nucleocytoplasmic shuttling. Dean, David A.; Urban, Gudrun; Aragon, Ileana V; Swingle, Mark; Miller, Beth; Rusconi, Sandro; Bueno, Manuel; Dean, Nicholas M.; Honkanen, Richard E. // BMC Cell Biology;2001, Vol. 2, p6 

    Background: In most cells glucocorticoid receptors (GR) reside predominately in the cytoplasm. Upon hormone binding, the GR translocates into the nucleus, where the hormone-activated GR-complex regulates the transcription of GR-responsive genes. Serine/threonine protein phosphatase type 5 (PP5)...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics