ERK1 and ERK2 MAPK are key regulators of distinct gene sets in zebrafish embryogenesis

Gabby Krens, S. F.; Corredor-Adámez, Maximiliano; Shuning He; Snaar-Jagalska, B. Ewa; Spaink, Herman P.
January 2008
BMC Genomics;2008, Vol. 9, Special section p1
Academic Journal
Background: The MAPK signaling proteins are involved in many eukaryotic cellular processes and signaling networks. However, specific functions of most of these proteins in vertebrate development remain elusive because of potential redundancies. For instance, the upstream activation pathways for ERK1 and ERK2 are highly similar, and also many of their known downstream targets are common. In contrast, mice and zebrafish studies indicate distinct roles for both ERKs in cellular proliferation, oncogenic transformation and development. A major bottleneck for further studies is that relatively little is known of in vivo downstream signaling specific for these kinases. Results: Microarray based gene expression profiling of ERK1 and ERK2 knockdown zebrafish embryos at various stages of early embryogenesis resulted in specific gene expression signature sets that showed pronounced differences in gene ontology analyses. In order to predict functions of these genes, zebrafish specific in silico signaling pathways involved in early embryogenesis were constructed using the GenMAPP program. The obtained transcriptome signatures were analyzed in the BMP, FGF, Nodal and Wnt pathways. Predicted downstream effects of ERK1 and ERK2 knockdown treatments on key pathways responsible for mesendoderm development were confirmed by whole mount in situ hybridization experiments. Conclusion: The gene ontology analyses showed that ERK1 and ERK2 target common and distinct gene sets, confirming the difference in knockdown phenotypes and diverse roles for these kinases during embryogenesis. For ERK1 we identified specific genes involved in dorsal-ventral patterning and subsequent embryonic cell migration. For ERK2 we identified genes involved in cell-migration, mesendoderm differentiation and patterning. The specific function of ERK2 in the initiation, maintenance and patterning of mesoderm and endoderm formation was biologically confirmed.


Related Articles

  • Distinct Roles of Two Zebrafish AHR Repressors (AHRRa and AHRRb) in Embryonic Development and Regulating the Response to 2,3,7,8-Tetrachlorodibenzo-p-dioxin. Matthew J. Jenny; Sibel I. Karchner; Diana G. Franks; Bruce R. Woodin; John J. Stegeman; Mark E. Hahn // Toxicological Sciences;Aug2009, Vol. 110 Issue 2, p426 

    The aryl hydrocarbon receptor (AHR) repressor (AHRR), an AHR-related basic helix-loop-helix/Per-AHR nuclear translocator-Sim protein, is regulated by an AHR-dependent mechanism and acts as a transcriptional repressor of AHR function. Resulting from a teleost-specific genome duplication,...

  • ISSUE HIGHLIGHTS.  // Genetics;Jun2014, Vol. 197 Issue 2, following p572 

    An overview of articles published in this issue of "Genetics" is presented including one on Y chromosome, one on zebrafish and one on mitogen-activated protein kinase.

  • Loss of eyes in zebrafish caused by mutation of chokh/rx3. Loosli, Felix; Staub, Wendy; Finger-Baier, Karin C.; Ober, Elke A.; Verkade, Heather; Wittbrodt, Joachim; Baier, Herwig // EMBO Reports;Sep2003, Vol. 4 Issue 9, p894 

    The vertebrate eye forms by specification of the retina anlage and subsequent morphogenesis of the optic vesicles, from which the neural retina differentiates. chokh (chk) mutant zebrafish lack eyes from the earliest stages in development. Marker gene analysis indicates that retinal fate is...

  • Screen of MicroRNA Targets in Zebrafish Using Heterogeneous Data Sources: A Case Study for Dre-miR-10 and Dre-miR-196. Yanju Zhang; Woltering, Joost M.; Verbeek, Fons J. // Proceedings of World Academy of Science: Engineering & Technolog;Dec2007, Vol. 36, p258 

    It has been established that microRNAs (miRNAs) play an important role in gene expression by post-transcriptional regulation of messengerRNAs (mRNAs). However, the precise relationships between microRNAs and their target genes in sense of numbers, types and biological relevance remain largely...

  • Parallel DNA pyrosequencing unveils new zebrafish microRNAs. Soares, Ana R.; Pereira, Patrícia M.; Santos, Bruno; Egas, Conceição; Gomes, Ana C.; Arrais, Joel; Oliveira, José L.; Moura, Gabriela R.; Santos, Manuel A. S. // BMC Genomics;2009, Vol. 10, Special section p1 

    Background: MicroRNAs (miRNAs) are a new class of small RNAs of approximately 22 nucleotides in length that control eukaryotic gene expression by fine tuning mRNA translation. They regulate a wide variety of biological processes, namely developmental timing, cell differentiation, cell...

  • T cell anergy is reversed by active Ras and is regulated by diacylglycerol kinase-α. Yuanyuan Zha; Marks, Reinhard; Ho, Allen W.; Peterson, Amy C.; Janardhan, Sujit; Brown, Ian; Praveen, Kesavannair; Stang, Stacey; Stone, James C.; Gajewski, Thomas F. // Nature Immunology;Nov2006, Vol. 7 Issue 11, p1166 

    T cell anergy has been correlated with defective signaling by the GTPase Ras, but causal and mechanistic data linking defective Ras activity with T cell anergy are lacking. Here we used adenoviral transduction to genetically manipulate nonproliferating T cells and show that active Ras restored...

  • Functional characterization of the zebrafish gadd45αb gene promoter and its application as a biosensor. Gireesh-Babu, P.; Pawar, Nilambari; Krishnan, P.; Kumar, A. Pavan; Zaidi, S. G. S.; Bhartiya, Deepa; Kumar, Neeraj; Sivasubbu, Sridhar; Rajendran, K. V.; Chaudhari, Aparna // Current Science (00113891);8/25/2012, Vol. 103 Issue 4, p388 

    We report characterization of zebrafish gadd45α b (growth arrest and DNA damage gene) minimal promoter and its application as biosensor tool for genotoxicity studies in zebrafish. Variably sized fragments of the 5' flanking region were generated using polymerase chain reaction (PCR). These...

  • RNA-Seq analysis in mutant zebrafish reveals role of U1C protein in alternative splicing regulation. Rösel, Tanja Dorothe; Hung, Lee-Hsueh; Medenbach, Jan; Donde, Katrin; Starke, Stefan; Benes, Vladimir; Rätsch, Gunnar; Bindereif, Albrecht // EMBO Journal;5/18/2011, Vol. 30 Issue 10, p1965 

    Precise 5′ splice-site recognition is essential for both constitutive and regulated pre-mRNA splicing. The U1 small nuclear ribonucleoprotein particle (snRNP)-specific protein U1C is involved in this first step of spliceosome assembly and important for stabilizing early splicing complexes....

  • Activation of MEK2 is sufficient to induce skin papilloma formation in transgenic zebrafish. Chih-Ming Chou; Yi-Chung Chen; San Su; Gen-Der Chen; Kai-Yun Huang; Huang-Wei Lien; Chang-Jen Huang; Chia-Hsiung Cheng // Journal of Biomedical Science;11/17/2015, Vol. 22, p1 

    Background: Mutations in mitogen-activated protein kinase (MAPK) kinase 1 (MEK1) that occur during cell proliferation and tumor formation are well described. Information on the roles of MEK2 in these effects is still limited. We established a constitutive MEK2 transgenic zebrafish,...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics