Comparative genomic analysis of Mycobacterium avium subspecies obtained from multiple host species

Paustian, Michael L.; Xiaochun Zhu; Sreevatsan, Srinand; Robbe-Austerman, Suelee; Kapur, Vivek; Bannantine, John P.
January 2008
BMC Genomics;2008, Vol. 9, Special section p1
Academic Journal
Background: Mycobacterium avium (M. avium) subspecies vary widely in both pathogenicity and host specificity, but the genetic features contributing to this diversity remain unclear. Results: A comparative genomic approach was used to identify large sequence polymorphisms among M. avium subspecies obtained from a variety of host animals. DNA microarrays were used as a platform for comparing mycobacterial isolates with the sequenced bovine isolate M. avium subsp. paratuberculosis (MAP) K-10. Open reading frames (ORFs) were classified as present or divergent based on the relative fluorescent intensities of the experimental samples compared to MAP K-10 DNA. Multiple large polymorphic regions were found in the genomes of MAP isolates obtained from sheep. One of these clusters encodes glycopeptidolipid biosynthesis enzymes which have not previously been identified in MAP. M. avium subsp. silvaticum isolates were observed to have a hybridization profile very similar to yet distinguishable from M. avium subsp. avium. Isolates obtained from cattle (n = 5), birds (n = 4), goats (n = 3), bison (n = 3), and humans (n = 9) were indistinguishable from cattle isolate MAP K-10. Conclusion: Genome diversity in M. avium subspecies appears to be mediated by large sequence polymorphisms that are commonly associated with mobile genetic elements. Subspecies and host adapted isolates of M. avium were distinguishable by the presence or absence of specific polymorphisms.


Related Articles

  • A Genomotaxonomy View of the Bradyrhizobium Genus. Ormeño-Orrillo, Ernesto; Martínez-Romero, Esperanza // Frontiers in Microbiology;6/13/2019, pN.PAG 

    Whole genome analysis of the Bradyrhizobium genus using average nucleotide identity (ANI) and phylogenomics showed the genus to be essentially monophyletic with seven robust groups within this taxon that includes nitrogen-fixing nodule forming bacteria as well as free living strains. Despite the...

  • Comparative genomics between human and animal associated subspecies of the Mycobacterium avium complex: a basis for pathogenicity. Timms, Verlaine J.; Hassan, Karl A.; Mitchell, Hazel M.; Neilan, Brett A. // BMC Genomics;9/15/2015, Vol. 16 Issue 1, p1 

    Background: A human isolate of Mycobacterium avium subsp. paratuberculosis (M. paratuberculosis 43525) was sequenced and compared genomically to other mycobacterial pathogens. M. paratuberculosis 43525 was recently isolated from a patient with ulcerative colitis and belongs to the M. avium...

  • Enumeration of Mycobacterium avium subsp. paratuberculosis by quantitative real-time PCR, culture on solid media and optical densitometry.  // BMC Research Notes;2012, Vol. 5 Issue 1, p114 

    The article presents information on a study which focuses on different methods used for determining the number of Mycobacterium avium subsp. paratuberculosis (MAP) cells in suspensions with the same optical densities (OD). It is said that most of the methods for determining the number of MAP...

  • Construction and screening of Mycobacterium paratuberculosis insertional mutant libraries. Cavaignac, Sonia M.; White, Stefan J.; de Lisle, Geoffrey W.; Collins, Desmond M. // Archives of Microbiology;Mar2000, Vol. 173 Issue 3, p229 

    Mycobacterium paratuberculosis causes Johne's disease, a common wasting disease in ruminants. As a first step in studying virulence mechanisms, libraries of random mutants were produced in two M. paratuberculosis strains by using the conditionally replicating shuttle phasmid phAE94 which...

  • Transposon display supports transpositional activity of P elements in species of the saltans group of Drosophila. De Setta, Nathalia; Costa, Ana Paula Pimentel; Lopes, Fabrício Ramon; Van Sluys, Marie-anne; Carareto, Cláudia Márcia Aparecida // Journal of Genetics;Apr2007, Vol. 86 Issue 1, p37 

    Mobilization of two P element subfamilies (canonical and O-type) from Drosophila sturtevanti and D. saltans was evaluated for copy number and transposition activity using the transposon display (TD) technique. Pairwise distances between strains regarding the insertion polymorphism profile were...

  • A large-scale study of differential gene expression in monocyte-derived macrophages infected with several strains of Mycobacterium avium subspecies paratuberculosis. Kabara, Edward; Kloss, Christopher C.; Wilson, Melind; Tempelman, Robert J.; Sreevatsan, Srinand; Janagama, Harish; Coussens, Paul M. // Briefings in Functional Genomics;May2010, Vol. 9 Issue 3, p220 

    Mycobacterium avium subspecies paratuberculosis (MAP) is a significant concern to the American and European dairy industries and possibly to human health. MAP possesses the rare ability to survive and replicate in infected macrophages, cells that are typically able to destroy pathogens. Little...

  • The Effect of Inactivated Mycobacterium Paratuberculosis Vaccine on the Response to a Heterologous Bacterial Challenge in Pigs. Jensen, Kristoffer Jarlov; Hansen, Mette Sif; Heegaard, Peter Mikael Helweg; Benn, Christine Stabell; Jungersen, Gregers // Frontiers in Immunology;7/5/2019, p1 

    Background: Vaccines may have non-specific effects, affecting resistance to heterologous pathogens. Veterinary vaccines have seldom been investigated for their non-specific effects. However, recent observational studies suggest that an inactivated paratuberculosis vaccine reduced all-cause...

  • Quantifying uncertainty in genotype calls. Carvalho, Benilton S.; Louis, Thomas A.; Irizarry, Rafael A. // Bioinformatics;Jan2010, Vol. 26 Issue 2, p242 

    Motivation: Genome-wide association studies (GWAS) are used to discover genes underlying complex, heritable disorders for which less powerful study designs have failed in the past. The number of GWAS has skyrocketed recently with findings reported in top journals and the mainstream media....

  • SNPchip: R classes and methods for SNP array data. Robert B. Scharpf; Jason C. Ting; Jonathan Pevsner; Ingo Ruczinski // Bioinformatics;Mar2007, Vol. 23 Issue 5, p627 

    Summary: High-density single nucleotide polymorphism microarrays (SNP chips) provide information on a subject's genome, such as copy number and genotype (heterozygosity/homozygosity) at a SNP. While fluorescence in situ hybridization and karyotyping reveal many abnormalities, SNP chips provide a...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics