TITLE

Comparative genomic analysis and evolution of the T cell receptor loci in the opossum Monodelphis domestica

AUTHOR(S)
Parra, Zuly E.; Baker, Michelle L.; Hathaway, Jennifer; Lopez, April M.; Trujillo, Jonathan; Sharp, Alana; Miller, Robert D.
PUB. DATE
January 2008
SOURCE
BMC Genomics;2008, Vol. 9, Special section p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: All jawed-vertebrates have four T cell receptor (TCR) chains: alpha (TRA), beta (TRB), gamma (TRG) and delta (TRD). Marsupials appear unique by having an additional TCR: mu (TRM). The evolutionary origin of TRM and its relationship to other TCR remain obscure, and is confounded by previous results that support TRM being a hybrid between a TCR and immunoglobulin locus. The availability of the first marsupial genome sequence allows investigation of these evolutionary relationships. Results: The organization of the conventional TCR loci, encoding the TRA, TRB, TRG and TRD chains, in the opossum Monodelphis domestica are highly conserved with and of similar complexity to that of eutherians (placental mammals). There is a high degree of conserved synteny in the genomic regions encoding the conventional TCR across mammals and birds. In contrast the chromosomal region containing TRM is not well conserved across mammals. None of the conventional TCR loci contain variable region gene segments with homology to those found in TRM; rather TRM variable genes are most similar to that of immunoglobulin heavy chain genes. Conclusion: Complete genomic analyses of the opossum TCR loci continue to support an origin of TRM as a hybrid between a TCR and immunoglobulin locus. None of the conventional TCR loci contain evidence that such a recombination event occurred, rather they demonstrate a high degree of stability across distantly related mammals. TRM, therefore, appears to be derived from receptor genes no longer extant in placental mammals. These analyses provide the first genomic scale structural detail of marsupial TCR genes, a lineage of mammals used as models of early development and human disease.
ACCESSION #
38122680

 

Related Articles

  • A VpreB3 homologue in a marsupial, the gray short-tailed opossum, Monodelphis domestica. Wang, Xinxin; Parra, Zuly; Miller, Robert // Immunogenetics;Aug2012, Vol. 64 Issue 8, p647 

    A VpreB surrogate light (SL) chain was identified for the first time in a marsupial, the opossum Monodelphis domestica. Comparing the opossum VpreB to homologues from eutherian (placental mammals) and avian species supported the marsupial gene being VpreB3. VpreB3 is a protein that is not known...

  • Convergent and divergent evolution of genomic imprinting in the marsupial Monodelphis domestica. Das, Radhika; Anderson, Nathan; Koran, MaryEllen I.; Weidman, Jennifer R.; Mikkelsen, Tarjei S.; Kamal, Michael; Murphy, Susan K.; Linblad-Toh, Kerstin; Greally, John M.; Jirtle, Randy L. // BMC Genomics;2012, Vol. 13 Issue 1, p394 

    Background: Genomic imprinting is an epigenetic phenomenon resulting in parent-of-origin specific monoallelic gene expression. It is postulated to have evolved in placental mammals to modulate intrauterine resource allocation to the offspring. In this study, we determined the imprint status of...

  • On the genomics of immunoglobulins in the gray, short-tailed opossum Monodelphis domestica. Xinxin Wang; Olp, Jonathan J.; Miller, Robert D. // Immunogenetics;Aug2009, Vol. 61 Issue 8, p581 

    Annotated maps of the IGH, IGK, and IGL loci in the gray, short-tailed opossum Monodelphis domestica were generated from analyses of the available whole genome sequence for this species. Analyses of their content and organization confirmed a number of previous conclusions based on...

  • Structure and expression of two nuclear receptor genes in marsupials: insights into the evolution of the antisense overlap between the a-thyroid hormone receptor and Rev-erbα. Rindfleisch, Brandon C.; Scott Brown, M.; VandeBerg, John L.; Munroe, Stephen H. // BMC Molecular Biology;2010, Vol. 11, p97 

    Background: Alternative processing of a-thyroid hormone receptor (TRα, NR1A1) mRNAs gives rise to two functionally antagonistic nuclear receptors: TRα1, the α-type receptor, and TRα2, a non-hormone binding variant that is found only in mammals. TRα2 shares an unusual antisense...

  • Recombination, transcription, and diversity of a partially germline-joined VH in a mammal. Wang, Xinxin; Miller, Robert // Immunogenetics;Sep2012, Vol. 64 Issue 9, p713 

    Full or partially germline-joined V genes have been described in a number of different vertebrate lineages where they can contribute to the expressed antibody repertoire through different mechanisms. Here we demonstrate that VH3.1, a partially germline-joined VH gene in the opossum Monodelphis...

  • In Vitro and In Silico Annotation of Conserved and Nonconserved MicroRNAs in the Genome of the Marsupial Monodelphis domestica. Devor, Eric J.; Samollow, Paul B. // Journal of Heredity;Jan/Feb2008, Vol. 99 Issue 1, p66 

    The gray short-tailed opossum, Monodelphis domestica, is the world's most widely utilized marsupial model for biomedical research. Recent completion of the initial M. domestica genome assembly offers the first opportunity to examine genome-wide phenomena in a marsupial. Using in silico methods,...

  • The Hunt. Devor, Eric J. // Drug Discovery & Development;Sep2008, Vol. 11 Issue 9, p26 

    The article reports on the development of a generic protocol for sequencing and cloning small RNAs by the Integrated DNA Technologies Inc. (IDT). The protocol enables the making of small RNA libraries from any main RNA source and records for the natural variability. IDT immediately identified...

  • Early Postnatal B Cell Ontogeny and Antibody Repertoire Maturation in the Opossum, Monodelphis domestica. Xinxin Wang; Sharp, Alana R.; Miller, Robert D.; Boudinot, Pierre // PLoS ONE;Sep2012, Vol. 7 Issue 9, Special section p1 

    Marsupials are a lineage of mammals noted for giving birth to highly altricial young, which complete much of their "fetal" development externally attached to a teat. Postnatal B cell ontogeny and diversity was investigated in a model marsupial species, the gray short-tailed opossum, Monodelphis...

  • Rsx is a metatherian RNA with Xist-like properties in X-chromosome inactivation. Grant, Jennifer; Mahadevaiah, Shantha K.; Khil, Pavel; Sangrithi, Mahesh N.; Royo, Hélène; Duckworth, Janine; McCarrey, John R.; VandeBerg, John L.; Renfree, Marilyn B.; Taylor, Willie; Elgar, Greg; Camerini-Otero, R. Daniel; Gilchrist, Mike J.; Turner, James M. A. // Nature;7/12/2012, Vol. 487 Issue 7406, p254 

    In female (XX) mammals, one of the two X chromosomes is inactivated to ensure an equal dose of X-linked genes with males (XY). X-chromosome inactivation in eutherian mammals is mediated by the non-coding RNA Xist. Xist is not found in metatherians (marsupials), and how X-chromosome inactivation...

Share

Read the Article

Courtesy of NEW JERSEY STATE LIBRARY

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics