New insights into the Saccharomyces cerevisiae fermentation switch: Dynamic transcriptional response to anaerobicity and glucose-excess

van den Brink, Joost; Daran-Lapujade, Pascale; Pronk, Jack T.; de Winde, Johannes H.
January 2008
BMC Genomics;2008, Vol. 9, Special section p1
Academic Journal
Background: The capacity of respiring cultures of Saccharomyces cerevisiae to immediately switch to fast alcoholic fermentation upon a transfer to anaerobic sugar-excess conditions is a key characteristic of Saccharomyces cerevisiae in many of its industrial applications. This transition was studied by exposing aerobic glucose-limited chemostat cultures grown at a low specific growth rate to two simultaneous perturbations: oxygen depletion and relief of glucose limitation. Results: The shift towards fully fermentative conditions caused a massive transcriptional reprogramming, where one third of all genes within the genome were transcribed differentially. The changes in transcript levels were mostly driven by relief from glucose-limitation. After an initial strong response to the addition of glucose, the expression profile of most transcriptionally regulated genes displayed a clear switch at 30 minutes. In this respect, a striking difference was observed between the transcript profiles of genes encoding ribosomal proteins and those encoding ribosomal biogenesis components. Not all regulated genes responded with this binary profile. A group of 87 genes showed a delayed and steady increase in expression that specifically responded to anaerobiosis. Conclusion: Our study demonstrated that, despite the complexity of this multiple-input perturbation, the transcriptional responses could be categorized and biologically interpreted. By comparing this study with public datasets representing dynamic and steady conditions, 14 up-regulated and 11 down-regulated genes were determined to be anaerobic specific. Therefore, these can be seen as true "signature" transcripts for anaerobicity under dynamic as well as under steady state conditions.


Related Articles

  • The effect of growth factors on anoxic chemostat cultures of two Saccharomyces cerevisiae strains. de Kock, S. H.; du Preez, J. C.; Kilian, S. G. // Biotechnology Letters;Jun2001, Vol. 23 Issue 12, p957 

    In anoxic chemostat cultures of Saccharomyces cerevisiae ATCC 4126 and CBS 8066 grown in a medium containing yeast extract, a sharp increase in the steady-state residual glucose concentration occurred at relatively low dilution rates, contrary to the expected Monod kinetics. However,...

  • Transcription of hexose transporters of Saccharomyces cerevisiae is affected by change in oxygen provision. Rintala, Eija; Wiebe, Marilyn G.; Tamminen, Anu; Ruohonen, Laura; Penttilä, Merja // BMC Microbiology;2008, Vol. 8, Special section p1 

    Background: The gene family of hexose transporters in Saccharomyces cerevisiae consists of 20 members; 18 genes encoding transporters (HXT1-HXT17, GAL2) and two genes encoding sensors (SNF3, RGT2). The effect of oxygen provision on the expression of these genes was studied in glucose-limited...

  • Increase of ethanol tolerance of Saccharomyces cerevisiae by error-prone whole genome amplification. Luhe, Annette Lin; Tan, Lily; Jinchuan Wu; Hua Zhao // Biotechnology Letters;May2011, Vol. 33 Issue 5, p1007 

    Saccharomyces cerevisiae was transformed for higher ethanol tolerance by error-prone whole genome amplification. The resulting PCR products were transformed back to the parental strain for homologous recombination to create a library of mutants with the perturbed genomic networks. A few rounds...

  • Integrated analysis of metabolic phenotypes in Saccharomyces cerevisiae. Duarte, Natalie C.; Palsson, Bernhard Ø.; Pengcheng Fu // BMC Genomics;2004, Vol. 5, p1 

    Background: The yeast Saccharomyces cerevisiae is an important microorganism for both industrial processes and scientific research. Consequently, there have been extensive efforts to characterize its cellular processes. In order to fully understand the relationship between yeast's genome and its...

  • Anomalies in the growth kinetics of Saccharomyces cerevisiae strains in aerobic chemostat cultures. de Kock, S H; du Preez, J C; Kilian, S G // Journal of Industrial Microbiology & Biotechnology;Apr2000, Vol. 24 Issue 4, p231 

    Aerobic glucose-limited chemostat cultivations were conducted with Saccharomyces cerevisiae strains NRRL Y132, ATCC 4126 and CBS 8066, using a complex medium. At low dilution rates all three strains utilised glucose oxidatively with high biomass yield coefficients, no ethanol production and very...

  • Inferring Metabolic States in Uncharacterized Environments Using Gene-Expression Measurements. Rossell, Sergio; Huynen, Martijn A.; Notebaart, Richard A. // PLoS Computational Biology;Mar2013, Vol. 9 Issue 3, p1 

    The large size of metabolic networks entails an overwhelming multiplicity in the possible steady-state flux distributions that are compatible with stoichiometric constraints. This space of possibilities is largest in the frequent situation where the nutrients available to the cells are unknown....

  • Analysis of the mechanism by which glucose inhibits maltose induction of MAL gene expression in... Zhen Hu; Yingzi Yue // Genetics;Jan2000, Vol. 154 Issue 1, p121 

    Studies the mechanism of glucose inhibition involved in maltose induction of the yeast Saccharomyces cerevisiae. Mig1 repressor's mediation of glucose repression of MAL gene expression; Relationship between maltose permease and glucose inhibition of maltose induction.

  • Functional analysis of human FEN1 in Saccharomyces cerevisiae and its role in genome stability. Greene, Amy L.; Snipe, Joyce R.; Gordenin, Dmitry A.; Resnick, Michael A. // Human Molecular Genetics;Nov99, Vol. 8 Issue 12, p2263 

    Analyzes the role of human flap endonuclease1 in genome stability of Saccharomyces cerevisiae. Proteins involved in DNA replication, recombination and repair; Possible consequences of changing the proliferating cell nuclear antigen-binding region; Effect of nuclease activity on growth inhibition.

  • Corrigendum.  // Genetics;Nov2013, Vol. 195 Issue 3, p1197 

    A correction to the article "Local Ancestry Corrects for Population Structure in Saccharomyces cerevisae Genome-Wide Association," by Liyang Diao and Kevin C. Chen published in the Volume 192 of "Genetics" is presented.


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics