Genome-wide analysis of the interaction between the endosymbiotic bacterium Wolbachia and its Drosophila host

Zhiyong Xi; Gavotte, Laurent; Yan Xie; Dobson, Stephen L.
January 2008
BMC Genomics;2008, Vol. 9, p1
Academic Journal
Background: Intracellular Wolbachia bacteria are obligate, maternally-inherited, endosymbionts found frequently in insects and other invertebrates. The success of Wolbachia can be attributed in part to an ability to alter host reproduction via mechanisms including cytoplasmic incompatibility (CI), parthenogenesis, feminization and male killing. Despite substantial scientific effort, the molecular mechanisms underlying the Wolbachia/host interaction are unknown. Results: Here, an in vitro Wolbachia infection was generated in the Drosophila S2 cell line, and transcription profiles of infected and uninfected cells were compared by microarray. Differentially-expressed patterns related to reproduction, immune response and heat stress response are observed, including multiple genes that have been previously reported to be involved in the Wolbachia/host interaction. Subsequent in vivo characterization of differentially-expressed products in gonads demonstrates that Angiotensin Converting Enzyme (Ance) varies between Wolbachia infected and uninfected flies and that the variation occurs in a sex-specific manner. Consistent with expectations for the conserved CI mechanism, the observed Ance expression pattern is repeatable in different Drosophila species and with different Wolbachia types. To examine Ance involvement in the CI phenotype, compatible and incompatible crosses of Ance mutant flies were conducted. Significant differences are observed in the egg hatch rate resulting from incompatible crosses, providing support for additional experiments examining for an interaction of Ance with the CI mechanism. Conclusion: Wolbachia infection is shown to affect the expression of multiple host genes, including Ance. Evidence for potential Ance involvement in the CI mechanism is described, including the prior report of Ance in spermatid differentiation, Wolbachia-induced sex-specific effects on Ance expression and an Ance mutation effect on CI levels. The results support the use of Wolbachia infected cell cultures as an appropriate model for predicting in vivo host/Wolbachia interactions.


Related Articles

  • ACE gene insertion/deletion polymorphism and renal scarring in children with urinary tract infections. Sekerli, Eleni; Katsanidis, Dimitrios; Vavatsi, Norma; Makedou, Areti; Gatzola, Magdalini // Pediatric Nephrology;Oct2009, Vol. 24 Issue 10, p1975 

    Urinary tract infection is a common bacterial disease that presents during childhood and may lead to renal scarring. Several studies have shown a strong association between the angiotensin converting enzyme (ACE) deletion polymorphism and renal scarring in children with vesicoureteric reflux...

  • The Drosophila immune response against Gram-negative bacteria is mediated by a peptidoglycan recognition protein. Gottar, Marie; Gobert, Vanessa; Michel, Tatiana; Belvin, Marcia; Duyk, Geoffrey; Hoffmann, Jules A.; Ferrandon, Dominique; Royet, Julien // Nature;4/11/2002, Vol. 416 Issue 6881, p640 

    The antimicrobial defence of Drosophila relies largely on the challenge-induced synthesis of an array of potent antimicrobial peptides by the fat body. The defence against Gram-positive bacteria and natural fungal infections is mediated by the Toll signalling pathway, whereas defence against...

  • Potential Deployment of Angiotensin I Converting Enzyme Inhibitors and of Angiotensin II Type 1 and Type 2 Receptor Blockers in Cancer Chemotherapy. Molteni, Agostino; Heffelfinger, Sue; Moulder, John E.; Uhal, Bruce; Castellani, William J. // Anti-Cancer Agents in Medicinal Chemistry;2006, Vol. 6 Issue 5, p451 

    There is significant evidence that both angiotensin I converting enzyme inhibitors (ACEI) and type 1 and type 2 angiotensin 2 (A2) receptor blockers may inhibit tumor growth. The finding is supported by many reports where these two classes of drugs showed cytostatic effects on the cultures of...

  • Wolbachia-Mediated Antibacterial Protection and Immune Gene Regulation in Drosophila. Wong, Zhee Sheen; Hedges, Lauren M.; Brownlie, Jeremy C.; Johnson, Karyn N. // PLoS ONE;2011, Vol. 6 Issue 9, p1 

    The outcome of microbial infection of insects is dependent not only on interactions between the host and pathogen, but also on the interactions between microbes that co-infect the host. Recently the maternally inherited endosymbiotic bacteria Wolbachia has been shown to protect insects from a...

  • What’s new in the renin-angiotensin system? Sturrock, E. D.; Natesh, R.; Van Rooyen, J. M.; Acharya, K. R. // Cellular & Molecular Life Sciences;Nov2004, Vol. 61 Issue 21, p2677 

    Angiotensin-converting enzyme (ACE) is a zinc- and chloride-dependent metallopeptidase that plays a vital role in the metabolism of biologically active peptides. Until recently, much of the inhibitor design and mechanism of action of this ubiquitous enzyme was based on the structures of...

  • Drosophila melanogaster cell culture as an experimental model to study recombination in Wolbachia pipientis. Goryacheva, I.; Gorelova, T.; Andrianov, B. // Russian Journal of Genetics;Dec2015, Vol. 51 Issue 12, p1159 

    Wolbachia pipientis is an obligate intracellular endosymbiont that commonly infects arthropods. Comparative genomic studies of Wolbachia reveal traces of numerous events of intergenic and intragenic recombination. The molecular mechanisms of recombination in Wolbachia are not currently known. We...

  • Bacterial strategies to overcome insect defences. Vallet-Gely, Isabelle; Lemaitre, Bruno; Boccard, Frédéric // Nature Reviews Microbiology;Apr2008, Vol. 6 Issue 4, p302 

    Recent genetic and molecular analyses have revealed how several strategies enable bacteria to persist and overcome insect immune defences. Genetic and genomic tools that can be used with Drosophila melanogaster have enabled the characterization of the pathways that are used by insects to detect...

  • Regulation of Inflammation and Myocardial Fibrosis in Experimental Autoimmune Myocarditis. Watanabe, Kenichi; Sukumaran, Vijayakumar; Veeraveedu, Punniyakoti T.; Thandavarayan, Rajarajan A.; Gurusamy, Narasimman; Ma, Meilei; Arozal, Wawaimuli; Sari, Flori R.; Lakshmanan, Arun Prasath; Arumugam, Somasundaram; Soetikno, Vivian; Rajavel, Varatharajan; Suzuki, Kenji // Inflammation & Allergy - Drug Targets;Jun2011, Vol. 10 Issue 3, p218 

    Autoimmune responses and inflammation are involved in the pathogenesis of many cardiovascular diseases. There is compelling evidence that inflammatory mechanisms may contribute to progressive heart failure. Thus, myocardial infiltration of lymphocytes and mononuclear cells, increased expression...

  • The carboxypeptidase ACE shapes the MHC class I peptide repertoire. Shen, Xiao Z; Billet, Sandrine; Lin, Chentao; Okwan-Duodu, Derick; Chen, Xu; Lukacher, Aron E; Bernstein, Kenneth E // Nature Immunology;Nov2011, Vol. 12 Issue 11, p1078 

    The surface presentation of peptides by major histocompatibility complex (MHC) class I molecules is critical to CD8+ T cell-mediated adaptive immune responses. Aminopeptidases have been linked to the editing of peptides for MHC class I loading, but carboxy-terminal editing is thought to be due...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics