Bioconjugation techniques for microfluidic biosensors

Goddard, Julie M.; Erickson, David
May 2009
Analytical & Bioanalytical Chemistry;May2009, Vol. 394 Issue 2, p469
Academic Journal
We have evaluated five bioconjugation chemistries for immobilizing DNA onto silicon substrates for microfluidic biosensing applications. Conjugation by organosilanes is compared with linkage by carbonyldiimidazole (CDI) activation of silanol groups and utilization of dendrimers. Chemistries were compared in terms of immobilization and hybridization density, stability under microfluidic flow-induced shear stress, and stability after extended storage in aqueous solutions. Conjugation by dendrimer tether provided the greatest hybridization efficiency; however, conjugation by aminosilane treated with glutaraldehyde yielded the greatest immobilization and hybridization densities, as well as enhanced stability to both shear stress and extended storage in an aqueous environment. Direct linkage by CDI activation provided sufficient immobilization and hybridization density and represents a novel DNA bioconjugation strategy. Although these chemistries were evaluated for use in microfluidic biosensors, the results provide meaningful insight to a number of nanobiotechnology applications for which microfluidic devices require surface biofunctionalization, for example vascular prostheses and implanted devices.


Related Articles

  • Planar Photonic Crystals Biosensor Applications of TiO2. ERDIVEN, U.; KARAASLAN, M.; UNAL, E.; KARADAĞ, F. // Acta Physica Polonica, A.;Oct2012, Vol. 122 Issue 4, p732 

    We examine quality factor and sensitivity change depending on the resonant wavelength changing re-fractive index of surrounding liquid for TiO2 photonic crystal slab structure. Photonic crystal slab structure is used widely for biological materials such as proteins, antigens, DNA, cells, virus...

  • Fabrication of a multiple-diameter branched network of microvascular channels with semi-circular cross-sections using xenon difluoride etching.  // Biomedical Microdevices;Apr2008, Vol. 10 Issue 2, p179 

    The majority of microfluidic devices employ networks of channels that have rectangular cross-sections. At the microvascular scale of 30 to 300 ìm in diameter, however, the distribution of fluid mechanical stresses and the induced shape of cultured cells will be quite different in a...

  • A synchronized quorum of genetic clocks. Danino, Tal; Mondragón-Palomino, Octavio; Tsimring, Lev; Hasty, Jeff // Nature;1/21/2010, Vol. 463 Issue 7279, p326 

    The engineering of genetic circuits with predictive functionality in living cells represents a defining focus of the expanding field of synthetic biology. This focus was elegantly set in motion a decade ago with the design and construction of a genetic toggle switch and an oscillator, with...

  • Electrokinetics-Based Microfluidic Technology for the Rapid Separation and Concentration of Bacteria/Cells/Biomolecules. I-Fang Cheng; Tzu-Ying Chen; Hsien-Chang Chang // Advanced Materials Research;2014, Issue 911, p347 

    Conventional techniques for detection of bacteria/cell and assessment of cancer cell typically use DNA techniques, Western blot and ELISA kits that are high cost, complicated processes and long time consuming. Our researches focus on rapid, portable, simple and highly sensitive separation and...

  • Frequency of Sperm DNA Fragmentation According to Selection Method: Comparison and Relevance of a Microfluidic Device and a Swim-up Procedure. KANAKO KISHI; HIROMI OGATA; SEIJI OGATA; YURI MIZUSAWA; ERI OKAMOTO; YUKIKO MATSUMOTO; SHOJI KOKEGUCHI; MASAHIDE SHIOTANI // Journal of Clinical & Diagnostic Research;Nov2015, Vol. 9 Issue 11, p14 

    Introduction: Multiple rounds of centrifugation or washing spermatozoa can cause sperm DNA fragmentation (SDF); however, a microfluidic approach to select spermatozoa does not require centrifugation. Reports have suggested that sperm sorting using a microfluidic device is an effective method to...

  • Fabrication of Microstructures Embedding Controllable Particles Inside Dielectrophoretic Microfluidic Devices. Tao Yue; Masahiro Nakajima; Hirotaka Tajima; Toshio Fukuda // International Journal of Advanced Robotic Systems;Feb2013, Vol. 10, p1 

    This paper presents a method of particle manipulation by dielectrophoresis (DEP) and immobilization using photo-crosslinkable resin inside microfluidic devices. High speed particle manipulation, including patterning and concentration control by DEP was demonstrated. Immovable and movable...

  • Fabrication of Glass-based Microfluidic Devices with Photoresist as Mask. Bahadorimehr, A.; Majlis, B. Y. // Electronics & Electrical Engineering;2011, Issue 116, p45 

    This paper presents a low cost method for etching of glass based microfluidic devices. Microchannels with the depth up to 150 µm were achieved by implementing a photoresist and wet etching process. In particular, a photoresist based mask method is introduced for glass etching which can...

  • Non-lithographic fabrication of metallic micromold masters by laser machining and welding. Shiu, Pun-Pang; Knopf, George; Ostojic, Mile; Nikumb, Suwas // International Journal of Advanced Manufacturing Technology;Mar2012, Vol. 59 Issue 1-4, p157 

    A non-lithographic process of rapidly fabricating metallic micromold masters for the manufacture of disposable polymer microfluidic devices is presented in this paper. The developed technique exploits the precision material removal capabilities of industrial lasers to cut accurate profiles of...

  • Fabrication and integration of microprism mirrors for high-speed three-dimensional measurement in inertial microfluidic system. Joonyoung Koh,; Jihye Kim; Shin, Jung H.; Wonhee Lee // Applied Physics Letters;9/15/2014, Vol. 105 Issue 11, p1 

    Inertial microfluidics utilizes fluid inertia from high flow velocity to manipulate particles and fluids in 3D. Acquiring a 3D information of particle positions and complex flow patterns within microfluidic devices requires 3D imaging techniques such as confocal microscopy, which are often...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics