TITLE

A novel approach for the detection of DNA using immobilized peptide nucleic acid (PNA) probes and signal enhancement by real-time immuno-polymerase chain reaction (RT-iPCR)

AUTHOR(S)
Roth, Lillian; Zagon, Jutta; Ehlers, Anke; Kroh, Lothar W.; Broll, Hermann
PUB. DATE
May 2009
SOURCE
Analytical & Bioanalytical Chemistry;May2009, Vol. 394 Issue 2, p529
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A new approach for the detection of DNA target molecules is described, using capture probes and subsequent signal enhancement by a uniform polymerase chain reaction (PCR). Peptide nucleic acid probes were immobilized in real-time PCR-compatible microtiter plates. After hybridization of biotinylated DNA targets, detection was performed by real-time immuno-PCR, a method formerly used for protein detection. We demonstrate the feasibility of this strategy for the qualitative detection of DNA oligonucleotides with a detection limit (LOD) of 6 attomol. Furthermore, the method was applied to PCR-amplified samples from genetically modified maize DNA (Mon810). A 483-bp DNA fragment was detected in mixture with 99.9% of noncomplementary DNA with a sensitivity down to the level of attomole. [Figure not available: see fulltext.]
ACCESSION #
37922847

 

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