TITLE

Homogeneous immunoassay based on gold nanoparticles and visible absorption detection

AUTHOR(S)
Anfossi, Laura; Baggiani, Claudio; Giovannoli, Cristina; Giraudi, Gianfranco
PUB. DATE
May 2009
SOURCE
Analytical & Bioanalytical Chemistry;May2009, Vol. 394 Issue 2, p507
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A sensitive homogeneous immunoassay, using human serum albumin (HSA) as a model analyte coupled with simple visible absorption detection, has been developed. The new assay is based on the use of gold nanoparticles functionalized with the target protein, which compete with the analyte for the binding of a specific polyclonal antibody. The binding of antibodies to the functionalized nanoparticles determines a shift of the visible absorption maximum of the gold colloid, and quantification of the analyte could be obtained as the competitive inhibition of the binding of antibodies to the nanoparticles. The proposed immunoassay has been optimized and successfully applied to measuring HSA in human urine samples, in which results agreed well with those obtained by a nephelometric reference method.
ACCESSION #
37922846

 

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