TITLE

Single-molecule immunosorbent assay as a tool for human immunodeficiency virus-1 antigen detection

AUTHOR(S)
Jiangwei Li; Wenjun Xie; Ning Fang; Yeung, Edward S.
PUB. DATE
May 2009
SOURCE
Analytical & Bioanalytical Chemistry;May2009, Vol. 394 Issue 2, p489
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Ultrasensitive detection and quantification of viral antigen with a novel single-molecule immunosorbent assay (SMISA) was achieved. Antigen from human immunodeficiency virus type 1 (HIV-1), the major etiological agent of acquired immune deficiency syndrome, served as the screening target in this study. The target molecule was sandwiched between a polyclonal capture antibody and a monoclonal detector antibody. The capture antibody was covalently immobilized on (3-glycidoxypropyl) trimethoxy silane-modified glass slides. The detector antibody was conjugated with fluorescent Alexa Fluor 532 labeled secondary antibody prior to being used as a probe for the antigen. Imaging was performed with a total internal reflection fluorescence single-molecule detection system. This technique is demonstrated for detecting HIV-1 p24 antigen down to 0.1 pg/mL with a dynamic range of over four orders of magnitude. A Langmuir isotherm fits the molecule count dependence on the target concentration. The target antigen was further tested in 20% human serum, and the results showed that neither sensitivity nor dynamic range was affected by the biological matrix. SMISA is therefore a promising approach for the early diagnosis of viral induced diseases.
ACCESSION #
37922845

 

Related Articles

  • Schistosomiasis and HIV in Rural Zimbabwe: Efficacy of Treatment of Schistosomiasis in Individuals with HIV Coinfection. Kallestrup, Per; Zinyama, Rutendo; Gomo, Exnevia; Butterworth, Anthony E.; van Dam, Govert J.; Gerstoft, Jan; Erikstrup, Christian; Ullum, Henrik // Clinical Infectious Diseases;6/15/2006, Vol. 42 Issue 12, p1781 

    Background. There is evidence from experimental models that the praziquantel-induced clearance of schistosomiasis is dependent on the host's immune response. Consequently, human immunodeficiency virus (HIV)-related immunodeficiency may impair the effect of praziquantel treatment. Methods. In a...

  • Comparison the Radiologic Findings of Pulmonary Tuberculosis Among HIV-Seropositive With HIV-Seronegative Patients. Ahmadi, Fatmeh; Salmanzadeh, Shokrollah; Kimyai, Mehdi // Jundishapur Journal of Microbiology;2012, Vol. 5 Issue 2, p421 

    Background: Approximately 1.8 million of the world's population is infected with both M.tuberculosis (TB) and HIV. HIV-seropositive patients with TB may have radiographically atypical presentations. Objectives: This study aimed to determine whether there was any difference in radiological...

  • Preface (Hot Topic: HIV Vaccines Executive Editor: Melissa Pope). Pope, Melissa // Current Molecular Medicine;May2003, Vol. 3 Issue 3, p1 

    Introduces a series of articles on the status of HIV vaccine research as of May 2003. Research status in terms of human and animal studies of putative vaccines; Identification of the types of immune responses that need to be elicited by the various strategies being considered to ensure vaccine...

  • CNE/CME questions.  // AIDS Alert;Oct2009, Vol. 24 Issue 10, p119 

    A quiz concerning human immunodeficiency virus (HIV) patients is presented.

  • Characterization of human anti-V3 monoclonal antibody 904 isolated from an Indian clade C Human Immunodeficiency Virus type-1 (HIV-1) infected donor. Andrabi, Raiees; Makhdoomi, Muzamil; Luthra, Kalpana // Retrovirology;2013, Vol. 10 Issue Suppl 1, p1 

    An abstract of the article "Characterization of human anti-V3 monoclonal antibody 904 isolated from an Indian clade C Human Immunodeficiency Virus type-1 (HIV-1) infected donor" by Raiees Andrabi, Muzamil Makhdoomi and Kalpana Luthra is presented.

  • gp41:. Huber, Jeffrey T.; Gillaspy, Mary L. // Encyclopedic Dictionary of AIDS-Related Terminology;2000, p100 

    An encyclopedia entry for "gp41," which refers to the transmembrane segment of the human immunodeficiency virus, is presented.

  • Subtle Mimicry of HLA by HIV-1 GP120 - A Role for Anti HLA Antibodies? Smith, Peter Lawrence; Dalgleish, Angus // Open Autoimmunity Journal;2010 Special Issue 2, p104 

    Human immunodeficiency virus (HIV-1) infection causes progressive immune deficiency by the targeted depletion of CD4+ T cells. The mechanism by which HIV-1 achieves this is not completely understood, however chronic immune activation is understood to be an essential feature of progressive HIV-1...

  • CCR6 Functions as a New Coreceptor for Limited Primary Human and Simian Immunodeficiency Viruses. Islam, Salequl; Shimizu, Nobuaki; Hoque, Sheikh Ariful; Jinno-Oue, Atsushi; Tanaka, Atsushi; Hoshino, Hiroo // PLoS ONE;Aug2013, Vol. 8 Issue 8, p1 

    More than 12 chemokine receptors (CKRs) have been identified as coreceptors for the entry of human immunodeficiency virus type 1 (HIV-1), type 2 (HIV-2), and simian immunodeficiency viruses (SIVs) into target cells. The expression of CC chemokine receptor 6 (CCR6) on Th17 cells and regulatory T...

  • Detection of Antibodies to Human Immunodeficiency Virus (HIV) That Recognize Conformational Epitopes of Glycoproteins 160 and 41 Often Allows for Early Diagnosis of HIV Infection. Jianmin Chen; Liqiang Wang; Chen, Jenny J.-Y.; Sahu, Gautam K; Tyring, Stephen; Ramsey, Keith; Indrikovs, Alexander J.; Petersen, John R.; Paar, David; Cloyd, Miles W. // Journal of Infectious Diseases;8/1/2002, Vol. 186 Issue 3, p321 

    On the basis of human immunodeficiency virus (HIV) needlestick studies, the time to seroconversion for anti-HIV antibodies is 1-9 months (mean, ∼2-3 months). However, an earlier marker of an immune response to HIV often occurs—serum anti-HIV antibodies reactive with live HIV-infected...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics