TITLE

DELVO-X-PRESS IR KITŲ METODŲ INHIBITORIAMS ŽALIAME PIENE NUSTATYTI PALYGINAMASIS ĮVERTINIMAS

AUTHOR(S)
Šalomskienė, Joana; Jonkuvienė, Dovilė; Paserpskienė, Marija
PUB. DATE
March 2009
SOURCE
Veterinarija ir Zootechnika;2009, Vol. 45 Issue 67, p73
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Comparison of four methods for determining inhibitors in raw milk - Delvotest-SP-NT (microbiological method with spores of Geobacillus stearothermophilus) and rapid methods Delvo-X-PRESS, Penzym and Penzym S - was made. It has been determined that sensitivity of Delvo-X-PRESS and Delvotest-SP-NT to four β-lactams (penicillin, ampicillin, amoxicyllin, oxacillin) corresponded with sensitivities declared by manufacturers. Comparison of rapid methods with a reference microbiological method for determining inhibitors in ‟positive„ milk samples resulted in 94.3 % coinciding of Delvo-X-PRESS and Delvotest-SP-NT, 73.7 % of Penzym and Delvotest-SP-NT and 68.0 % of Penzym S and Delvotest-SP-NT, respectively. Comparison of two rapid methods widely used in Lithuania for determining inhibitors in milk - Delvo-X-PRESS and Penzym - showed that 68.4 % results of investigated samples tallied. Examination of milk samples in which antibacterial substances were found only using Penzym and Penzym S method and also the samples, in which antibacterial substances have been found using Delvotest-SP-NT, Penzym and Penzym S method after treating them with penicilinase and p-aminobenzoic acid showed that the results received using Penzym and Penzym S methods could be false positive. Based on the results of investigation, proposition was made to validate Delvo-X-PRESS method which results during investigation of ‟positive„ milk samples tallied with a microbiological method in 94.3 % cases.
ACCESSION #
37836132

 

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