Aberrant Methylation of the Netrin-1 Receptor Genes UNC5C and DCC Detected in Advanced Colorectal Cancer

Hibi, Kenji; Mizukami, Hiroki; Shirahata, Atsushi; Goto, Tetsuhiro; Sakata, Makiko; Sanada, Yutaka
May 2009
World Journal of Surgery;May2009, Vol. 33 Issue 5, p1053
Academic Journal
UNC5C and DCC, the netrin-1 receptors, belong to the functional dependence receptors family, which shares the ability to induce apoptosis in the absence of their ligands. Recently, two reports indicated that UNC5C and DCC methylation was closely associated with loss of gene expression in colorectal cancer. These results prompted us to examine the methylation status of the UNC5C and DCC genes in the colorectal carcinomas we surgically removed. The methylation status of the UNC5C and DCC genes were examined in primary carcinomas and the corresponding normal tissues derived from 50 patients with colorectal cancer using quantitative methylation-specific polymerase chain reaction (qMSP). The correlation between the methylation status and the clinicopathologic findings was then evaluated. Aberrant methylation of the netrin-1 receptor genes were detected in 41 of the 50 (82%) primary colon cancers, suggesting that the aberrant methylation of netrin-1 receptors was frequently observed in colorectal cancer. The clinicopathologic data were then correlated with this result. A significant difference was observed in the Dukes stage ( p = 0.0438). Netrin-1 receptors might act as a tumor suppressor in colorectal cancers, and thus methylation might present a malignant potential in colorectal cancer.


Related Articles

  • Increased LEF1 Expression and Decreased Notch2 Expression Are Strong Predictors of Poor Outcomes in Colorectal Cancer Patients. Wen-Juan Wang; Yu Yao; Li-Li Jiang; Ting-Hua Hu; Jie-Qun Ma; Zhi-Ping Ruan; Tao Tian; Hui Guo; Shu-Hong Wang; Ke-Jun Nan // Disease Markers;2013, Vol. 35 Issue 5, p395 

    Background/Objective. We aimed to examine the expression of lymphoid enhancer factor 1 (LEF1) and Notch2 in colorectal cancer (CRC) and their association with clinicopathologic variables and CRC patients' prognosis. Methods. Immunohistochemistry, quantitative real-time polymerase chain reaction...

  • Vitamin D receptor haplotypes protect against development of colorectal cancer. Flügge, Jana; Krusekopf, Solveigh; Goldammer, Mark; Osswald, Elena; Terhalle, Werner; Malzahn, Uwe; Roots, Ivar // European Journal of Clinical Pharmacology;Nov2007, Vol. 63 Issue 11, p997 

    We investigated the association of vitamin D receptor polymorphisms and colorectal cancer incidence in a Caucasian population. Frequencies of the vitamin D receptor gene polymorphisms 23005G>A (CDX-2), 27823C>T (FokI), 60890G>A (BsmI), 61050G>A (Tru9I), 61888G>T (ApaI), and 61968T>C (TaqI) were...

  • The frequency of human papillomaviruses in colorectal cancer samples in Mashhad, northeastern Iran. MESHKAT, Mojtaba; TAYYEBI MEIBODI, Naser; SEPAHI, Samaneh; FADAEE, Narges; SALEHPOUR, Masoumeh; MESHKAT, Zahra // Turkish Journal of Medical Sciences;2014, Vol. 44 Issue 3, p501 

    Background/aim: Infection with the human papillomaviruses (HP Vs) is associated with the development of several cancers, including oral, esophageal, skin, lung, and cervical. However, the association of HPVs and colorectal cancers remains controversial. The aim of this study was to evaluate the...

  • Low expression of chemokine receptor CCR5 in human colorectal cancer correlates with lymphatic dissemination and reduced CD8+ T-cell infiltration. Zimmermann, Tim; Moehler, Markus; Gockel, Ines; Sgourakis, George; Biesterfeld, Stefan; Müller, Michaela; Berger, Martin; Lang, Hauke; Galle, Peter; Schimanski, Carl // International Journal of Colorectal Disease;Apr2010, Vol. 25 Issue 4, p417 

    Chemokines and their receptors have been proposed to distinctly contribute to tumor growth, dissemination, and local immune escape. The aim of this study was to evaluate the relevance of the chemokine receptor CCR5 expression for the progression of human colorectal cancer. CCR5 expression was...

  • Activating K-Ras mutations outwith 'hotspot' codons in sporadic colorectal tumours - implications for personalised cancer medicine. Smith, G.; Bounds, R.; Wolf, H.; Steele, R. J. C.; Carey, F. A.; Wolf, C. R. // British Journal of Cancer;2/15/2010, Vol. 102 Issue 4, p693 

    Background: Response to EGFR-targeted therapies in colorectal cancer patients has been convincingly associated with Kirsten-Ras (K-Ras) mutation status. Current mandatory mutation testing for patient selection is limited to the K-Ras 'hotspot' codons 12 and 13.Methods:...

  • Serum expression levels of miR-17, miR-21, and miR-92 as potential biomarkers for recurrence after adjuvant chemotherapy in colon cancer patients. Conev, Nikolay V.; Donev, Ivan S.; Konsoulova-Kirova, Assia A.; Kashlov, Javor K.; Chervenkov, Trifon G.; Ivanov, Krasimir D. // BioScience Trends;Dec2015, Vol. 9 Issue 6, p393 

    The present study examined whether miR-17, miR-21, miR-29a, and miR-92 that are dysregulated in colon cancer (CC) can serve as potential predictive markers for relapse of disease after radical surgery and adjuvant chemotherapy. Real-time reverse transcription quantitative polymerase chain...

  • Exosomal microRNA in serum is a novel biomarker of recurrence in human colorectal cancer. Matsumura, T; Sugimachi, K; Iinuma, H; Takahashi, Y; Kurashige, J; Sawada, G; Ueda, M; Uchi, R; Ueo, H; Takano, Y; Shinden, Y; Eguchi, H; Yamamoto, H; Doki, Y; Mori, M; Ochiya, T; Mimori, K // British Journal of Cancer;7/14/2015, Vol. 113 Issue 2, p275 

    Background:Functional microRNAs (miRNAs) in exosomes have been recognised as potential stable biomarkers in cancers. The aim of this study is to identify specific miRNAs in exosome as serum biomarkers for the early detection of recurrence in human colorectal cancer (CRC).Methods:Serum samples...

  • Detection of mismatch repair gene germline mutation carrier among Chinese population with colorectal cancer. Hei-Ying Jin; Xiufang Liu; Ka Ming Li, Vicky; Yijiang Ding; Bolin Yang; Jianxiang Geng; Rensheng Lai; Shuqing Ding; Min Ni; Ronghua Zhao // BMC Cancer;2008, Vol. 8, Special section p1 

    Background: Hereditary nonpolyposis colorectal cancer (HNPCC) is an autosomal dominant syndrome. The National Cancer Institute (NCI) has recommended the Revised Bethesda guidelines for screening HNPCC. There has been a great deal of research on the value of these tests in other countries....

  • Low level microsatellite instability may be associated with reduced cancer specific survival in sporadic stage C colorectal carcinoma. Wright, C. M.; Dent, O. F.; Newlond, R. C.; Barker, M.; Chapuis, P. H.; Bokey, E. L.; Young, J. P.; Leggelt, B. A.; Jass, J. R.; Macdonald, G. A. // Gut;Jan2005, Vol. 54 Issue 1, p103 

    Background: Colorectal cancers (CRCs) may be categorised according to the degree of microsatellite instability (MSI) exhibited, as MSI-high (MSI-H), MSI-low (M5I-L), or microsatellite stable (MSS). MSI-H status confers a survival advantage to patients with sporadic CRC. Aims: To determine if low...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics