DNA methyltransferase expression in the human endometrium: down-regulation by progesterone and estrogen

Yoshiaki Yamagata; Hiromi Asada; Isao Tamura; Lifa Lee; Ryo Maekawa; Ken Taniguchi; Toshiaki Taketani; Aki Matsuoka; Hiroshi Tamura; Norihiro Sugino
May 2009
Human Reproduction;May2009, Vol. 24 Issue 5, p1126
Academic Journal
BACKGROUND Epigenetic regulation may be involved in modulation of gene expression during the normal cyclic changes of the human endometrium. We investigated expression of DNA methyltransferases (DNMTs) in endometrium during the menstrual cycle and the influence of sex steroid hormones on DNMT in endometrial stromal cells (ESC) in culture. METHODS Expression of DNMT1, DNMT3a and DNMT3b was assessed by immunohistochemistry and real-time RT–PCR in endometrial tissue (n = 42 women). ESC (n = 3 women) were cultured with estradiol and medroxyprogesterone acetate (E MPA) for 17 days, and DNMT mRNA levels were measured by real-time RT–PCR. RESULTS Nuclei of both epithelial and stromal cells immunostained for DNMT1, DNMT3a and DNMT3b during each phase of the menstrual cycle. Tissue levels of DNMT1 and DNMT3a mRNA were significantly lower in the mid-secretory phase than in the proliferative phase (P P P CONCLUSIONS DNMT mRNAs declined in the human endometrium during the secretory phase, and E MPA down-regulated DNMT3a and DNMT3b mRNAs in ESC in culture. These results suggest that DNMTs have regulatory functions in gene expression that is associated with decidualization.


Related Articles

  • Expression of Transcription Factors in Endometrium During Natural Cycles. Maldonado, Vicente; Castilla, José Antonio; Martínez, Luis; Herruzo, Alfonso; Concha, Ángel; Fontes, Juan; Mendoza, Nicolás; García-Peña, María Luisa; Mendoza, José Luis; Magán, Rosa; Ortiz, Águeda; González, Esther // Journal of Assisted Reproduction & Genetics;Nov2003, Vol. 20 Issue 11, p474 

    Purpose: The sex steroid control of the endometrial cycle is mediated by transcription factors, four of which are the estrogen and progesterone receptors, c-jun and c-fos, all expressed by the endometrium. The aim of this study was to analyze the distribution of the transcription factors in the...

  • Quantitative histomorphometric analysis of gonadal steroid receptor distribution in the normal human endometrium through the menstrual cycle. Taylor, Anthony; Guzail, Muna; Wahab, May; Thompson, John; Al-Azzawi, Farook // Histochemistry & Cell Biology;Apr2005, Vol. 123 Issue 4/5, p463 

    The aim of this study was to test the hypothesis that the distribution of oestrogen receptor beta (ERβ) and androgen receptor (AR) are related to cell proliferation or correlated with the expression of progesterone receptor (PR) or oestrogen receptor alpha (ERα) in the normal human...

  • Regulation in the Endometrium.  // Fertility Weekly;6/22/2009, p7 

    The article discusses a study conducted by researcher Y. Yamagata and colleagues regarding expression of DNA methyltransferases (DNMTs) regulation in the human endometrium during the menstrual cycle. The study also assesses the influence of sex steroid hormones on DNMT. The study was based on 42...

  • Molecular events in the endometrium. Oestrogen receptor α and β mRNA expression in human endometrium throughout the menstrual cycle. Matsuzaki, Sachiko; Fukaya, Takao; Suzuki, Takashi; Murakami, Takashi; Sasano, Hironobu; Yajima, Akira // MHR: Basic Science of Reproductive Medicine;Jun99, Vol. 5 Issue 6, p559 

    We examined the localization of oestrogen receptor (ER) β mRNA in the human endometrium throughout the menstrual cycle using non-radioactive in-situ hybridization with Brigati-tailed oligonucleotides. The findings were compared with those of ERα in order to examine the possible biological...

  • Gene expression profile of human endometrial receptivity: comparison between natural and stimulated cycles for the same patients. Haouzi, D.; Assou, S.; Mahmoud, K.; Tondeur, S.; Rème, T.; Hedon, B.; De Vos, J.; Hamamah, S. // Human Reproduction;Jun2009, Vol. 24 Issue 6, p1436 

    : BACKGROUND The adjunction of exogenous hormones for controlled ovarian stimulation (COS) may alter endometrial receptiveness. In order to identify the genes misregulated under COS, we compared the endometrium gene expression profiles, from the same patients, in a natural cycle and in a...

  • Estrogen stimulates the human endometrium to express a factor(s) that promotes vascular smooth muscle cell migration as an early step in microvessel remodeling. Jeffery Babischkin; Thomas Bonagura; Laurence Udoff; Christine Vergara; Harry Johnson; Robert Atlas; Gerald Pepe; Eugene Albrecht // Endocrine (1355008X);Feb2009, Vol. 35 Issue 1, p81 

    Abstract  Vascular smooth muscle cell (VSMC) migration is a pivotal early step in blood vessel remodeling; however, very little is known about the regulation of this process in the human endometrium during the menstrual cycle. In this study, explants of human endometrium were incubated...

  • Estrogen and progesterone regulate expression of the endothelins in the rhesus macaque endometrium. Keator, Christopher S.; Mah, Kuni; Ohm, Lindsay; Slayden, Ov D. // Human Reproduction;Jul2011, Vol. 26 Issue 7, p1715 

    Background: Endothelins (EDNs) are thought to modulate endometrial blood flow during menses, stromal healing and endometrial growth during the proliferative phase. Our goal was to assess the effects of estrogen and progesterone on the EDN paracrine system in the endometrium of...

  • Urinary estrogen and progesterone metabolite concentrations in menstrual cycles of fertile women with non-conception, early pregnancy loss or clinical pregnancy. Scott A. Venners; Xue Liu; Melissa J. Perry; Susan A. Korrick; Zhiping Li; Fan Yang; Jianhua Yang; Bill L. Lasley; Xiping Xu; Xiaobin Wang // Human Reproduction;Sep2006, Vol. 21 Issue 9, p2272 

    BACKGROUND: Knowledge is limited of how estrogen and progesterone variability in fertile women are associated with achieving pregnancy. METHODS: From 1996 to 1998, we enrolled 347 textile workers without hormone treatment in Anhui, China, who provided daily urine and data upon stopping...

  • Effects of estrogen and progesterone on female knee joint laxity. Heitz, N.A.; Eisenman, P.A. // Journal of Athletic Training (National Athletic Trainers' Associ;Apr-Jun98 Supplement, Vol. 33 Issue 2, pS42 

    Presents a study which examined whether female athletes experienced more knee laxity in conjunction with estrogen and progesterone surges, in a 28-30 day menstrual cycle. Comparison of the incidence of anterior cruciate ligament injury in female and male athletes; Details on the measuring of...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics