TITLE

The BAG-1 cochaperone is a negative regulator of p73-dependent transcription

AUTHOR(S)
Wang, X.-H.; O'Connor, D.; Brimmell, M.; Packham, G.
PUB. DATE
April 2009
SOURCE
British Journal of Cancer;4/21/2009, Vol. 100 Issue 8, p1347
SOURCE TYPE
Academic Journal
DOC. TYPE
journal article
ABSTRACT
High-level expression of Bcl-2 associated athanogene (BAG-1) protects cancer cells from stress-induced cell death and growth inhibition. These protective effects of BAG-1 are dependent on interactions with the HSC70 and HSP70 chaperones. However, the key stress-response molecules that are regulated by a BAG-1/chaperone mechanism have not been identified. In this study, we investigated the effects of BAG-1 overexpression on the function of p53 family proteins, p53, p63 and p73. Overexpression of BAG-1 isoforms interfered with the transactivating activity of p73 and p63, but had modest and variable effects on p53-dependent transcription. p73 and BAG-1 interacted in intact cells and overexpression of BAG-1 decreased the expression of p73. siRNA-mediated ablation of endogenous BAG-1 increased the activity of a p73-responsive promoter and this was reversed by knock-down of p73. The ability of BAG-1 to modulate p73 activity and expression, and to interact with p73 were dependent on amino acid residues required for the interaction of BAG-1 with HSC70 and HSP70. These results show that BAG-1 inhibits the transactivating functions of p73 and provide new insight into the mechanisms that control the expression of p73. Inhibition of p73 function may be one mechanism that contributes to the pro-survival activity of BAG-1.
ACCESSION #
37591422

 

Related Articles

  • T(3;7)(q27;q32) fuses BCL6 to a non-coding region at FRA7H near miR-29. Schneider, B.; Nagel, S.; Kaufmann, M.; Winkelmann, S.; Bode, J.; Drexler, H. G.; MacLeod, R. A. F. // Leukemia (08876924);Jun2008, Vol. 22 Issue 6, p1262 

    A letter to the editor is presented in response to the article "t(3;7)(q27;q32) Fuses BCL6 to a Non-Coding Region at FRA7H Near miR-29," in the November 8, 2007 issue.

  • Endogenous expression of the atypical chemokine receptor CCX-CKR (CCRL1) gene in human embryonic kidney (HEK 293) cells. Salimi, Parvin; Esmaeili, Abolghasem; Hashemi, Mohammad; Behjati, Mohaddeseh // Molecular & Cellular Biochemistry;Jan2016, Vol. 412 Issue 1/2, p229 

    CCX-CKR (CCRL1) as one of the chemokine receptor-like proteins is a scavenger of CCL19, CCL21, CCL25, and CXCL13 chemokines. Human CCX-CKR is expressed in various tissues. Since HEK 293 cells are used for both transient and stable expression of CCX-CKR gene, it is important to determine...

  • The fusidic acid stimulon of Staphylococcus aureus. Delgado, Alejandro; Zaman, Shahrear; Muthaiyan, Arunachalam; Nagarajan, Vijayaraj; Elasri, Mohamed O; Wilkinson, Brian J; Gustafson, John E // Journal of Antimicrobial Chemotherapy (JAC);Dec2008, Vol. 62 Issue 6, p1207 

    Objectives: Fusidic acid interferes with the release of elongation factor G (EF-G) after the translocation step of protein synthesis. The objective of this study was to characterize the fusidic acid stimulon of a fusidic acid-susceptible strain of Staphylococcus aureus...

  • Enhanced upregulation of smooth muscle related transcripts by TGF beta2 in asthmatic (myo) fibroblasts. Wicks, J.; Haitchi, H. M.; Holgate, S. I.; Davies, D. E.; Powell, R. M.; Holgate, S T // Thorax;Apr2006, Vol. 61 Issue 4, p313 

    Background: Transforming growth factor beta (TGF beta) upregulates a number of smooth muscle specific genes in (myo)fibroblasts. As asthma is characterised by an increase in airway smooth muscle, we postulated that TGFbeta(2) favours differentiation of asthmatic (myo)fibroblasts...

  • Novel Roles for Notch3 and Notch4 Receptors in Gene Expression and Susceptibility to Ozone-Induced Lung Inflammation in Mice. Verhein, Kirsten C.; McCaw, Zachary; Gladwell, Wesley; Trivedi, Shweta; Bushel, Pierre R.; Kleeberger, Steven R. // Environmental Health Perspectives;Aug2015, Vol. 123 Issue 8, p799 

    Background: Ozone is a highly toxic air pollutant and global health concern. Mechanisms of genetic susceptibility to ozone-induced lung inflammation are not completely understood. We hypothesized that Notch3 and Notch4 are important determinants of susceptibility to ozoneinduced lung...

  • Molecular cloning of heat shock protein 60 ( PtHSP60) from Portunus trituberculatus and its expression response to salinity stress. Xu, Qianghua; Qin, Ye // Cell Stress & Chaperones;Winter2012, Vol. 17 Issue 5, p589 

    Heat shock protein 60 (HSP60) is a highly conserved and multi-functional molecular chaperone that plays an essential role in both cellular metabolism and stress response. Portunus trituberculatus is an important marine fishery and aquaculture species, and water salinity condition influenced its...

  • Estrogen potentiates the combined effects of transforming growth factor-beta and tumor necrosis factor-alpha on adult human osteoblast-like cell prostaglandin E2 biosynthesis. Secreto, F J; Grover, A; Pacurari, M; Rice, M B; Kantorow, M; Bidwai, A P; Blaha, J D; Keeting, P E // Calcified Tissue International;Dec2003, Vol. 73 Issue 6, p565 

    Reports that estrogen treatment modulates arachidonic acid metabolism by bone and bone cells are found in the literature. However, conflicting indications of the relationship that exists between estrogen and arachidonic acid metabolism emerge from the analysis of those reports. The present...

  • Molecular cloning and sexually dimorphic expression of DMRT4 gene in Oreochromis aureus. Cao, Jinling; Chen, Jianjie; Wu, Tingting; Gan, Xi; Luo, Yongju // Molecular Biology Reports;Jul2010, Vol. 37 Issue 6, p2781 

    The DM-domain gene family has at least eight members with conserved DNA-binding DM-domain, which encodes putative transcription factors related to the sexual regulator Dsx of Drosophila and Mab-3 of C. elegans. Although some of the DM genes are involved in sexual development, the function of...

  • MiniSOX9, a dominant-negative variant in colon cancer cells. Abdel-Samad, R; Zalzali, H; Rammah, C; Giraud, J; Naudin, C; Dupasquier, S; Poulat, F; Boizet-Bonhoure, B; Lumbroso, S; Mouzat, K; Bonnans, C; Pignodel, C; Raynaud, P; Fort, P; Quittau-Prévostel, C; Blache, P // Oncogene;6/2/2011, Vol. 30 Issue 22, p2493 

    Inherited and acquired changes in pre-mRNA processing have significant roles in human diseases, especially cancer. Characterization of aberrantly spliced mRNAs may thus contribute to understand malignant transformation. We recently reported an anti-oncogenic potential for the SOX9 transcription...

  • siRNA-mediated inhibition of Na(+)-K(+)-2Cl- cotransporter (NKCC1) and regulatory volume increase in the chondrocyte cell line C-20/A4. Qusous, Ala; Geewan, Corinne; Greenwell, Pamela; Kerrigan, Mark; Geewan, Corinne S V; Kerrigan, Mark J P // Journal of Membrane Biology;Oct2011, Vol. 243 Issue 1-3, p25 

    The Na(+)-K(+)-2Cl(-) cotransporter (NKCC1) is an essential membrane transporter and has been linked to the regulation of volume, matrix synthesis and bone growth in chondrocytes; the sole resident cell type of articular cartilage. Despite the integral nature of NKCC1, its regulation is...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics