TITLE

Effect of clenbuterol administration on the healthy murine heart

AUTHOR(S)
Moore, D.; Anderson, M.; Larson, D. F.
PUB. DATE
September 2008
SOURCE
Perfusion;Sep2008, Vol. 23 Issue 5, p297
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Clenbuterol has recently been shown to reverse pathologic cardiac remodeling during left ventricular assist device (LVAD) support, leading to restored ventricular function and explantation of LVAD devices. However, others have not been able to support these observations. Our hypothesis is that the β2-adrenergic activity of clenbuterol induces cardiac extracellular matrix (ECM) remodeling, resulting in increased interstitial fibrillar collagen content and altered diastolic function that may account for these conflicting reports. The intent of this study is to characterize the effect of clenbuterol on healthy murine hearts with transthoracic echo and histology. C57BL/6 female mice were administered 2.4 μg/kg/day of clenbuterol in the drinking water for 7 days and analysis conducted on day 8-24 hours after the last dose of clenbuterol. Histological analysis demonstrated an increase in left ventricular ECM collagen content in a control group compared with the clenbuterol group (density 0.32 ± 0.16 compared to 2.01 ± 0.30 RD/mm2). The ventricular fibrosis was supported by altered diastolic function measured by transthoracic echo where there was a significant increase in isovolumic relaxation time, and left atrial dimension and a decrease in left ventricular free wall tissue Doppler ratios. Our study showed no significant differences in left ventricular ejection fraction, cardiac output, or heart rate between the clenbuterol and control groups. These data suggest that the β-2 adrenergic activity of clenbuterol increases ECM fibrillar collagen concentrations in normal hearts, resulting in altered diastolic function.
ACCESSION #
37557350

 

Related Articles

  • Advances in Optical Coherence Tomography Imaging for Dermatology. Pierce, Mark C.; Strasswimmer, John; Park, B. Hyle; Cense, Barry; de Boer, Johannes F. // Journal of Investigative Dermatology;Sep2004, Vol. 123 Issue 3, p458 

    Optical coherence tomography (OCT) is a non-invasive imaging technique, which has previously demonstrated potential for use in dermatology. The purpose of this study is to demonstrate how improvements in image quality, speed, and functionality enable qualitative and quantitative information to...

  • Hemodynamic and Metabolic Parameters During Prolonged Cardiac Arrest and Reperfusion by Extracorporeal Circulation. MLČEK, M.; OŠŤÁDAL, P.; BĚLOHLÁVEK, J.; HAVRÁNEK, Š.; HRACHOVINA, M.; HUPTYCH, M.; HÁLA, P.; HRACHOVINA, V.; NEUŽIL, P.; KITTNAR, O. // Physiological Research;2012 Supplement 2, Vol. 61 Issue s2, pS57 

    Extracorporeal membranous oxygenation (ECMO) is increasingly used in the management of refractory cardiac arrest. Our aim was to investigate early effects of ECMO after prolonged cardiac arrest. In fully anesthetized swine (48 kg, N=18) ventricular fibrillation (VF) was induced and untreated...

  • Spatiotemporally Controlled Cardiac Conduction Block Using High-Frequency Electrical Stimulation. Dura, Burak; Kovacs, Gregory T. A.; Giovangrandi, Laurent // PLoS ONE;Apr2012, Vol. 7 Issue 4, p1 

    Background: Methods for the electrical inhibition of cardiac excitation have long been sought to control excitability and conduction, but to date remain largely impractical. High-amplitude alternating current (AC) stimulation has been known to extend cardiac action potentials (APs), and has been...

  • Spatiotemporally Controlled Cardiac Conduction Block Using High-Frequency Electrical Stimulation. Dura, Burak; Kovacs, Gregory T. A.; Giovangrandi, Laurent // PLoS ONE;Apr2012, Vol. 7 Issue 4, p1 

    Background: Methods for the electrical inhibition of cardiac excitation have long been sought to control excitability and conduction, but to date remain largely impractical. High-amplitude alternating current (AC) stimulation has been known to extend cardiac action potentials (APs), and has been...

  • Cortical and Putamen Age-Related Changes in the Microvessel Density and Astrocyte Deficiency in Spontaneously Hypertensive and Stroke-Prone Spontaneously Hypertensive Rats. Ritz, Marie-Françoise; Fluri, Felix; Engelter, Stefan T.; Schaeren-Wiemers, Nicole; Lyrer, Philippe A. // Current Neurovascular Research;Nov2009, Vol. 6 Issue 4, p279 

    Cerebral small vessel disease (SVD) is a major contributor to dementia in the elderly, and hypertension represents a major cause for developing the disease. However, little is known about its development and progression. Modifications of large cerebral arteries due hypertension are thought to...

  • A polyconvex hyperelastic model for fiber-reinforced materials in application to soft tissues. Ehret, Alexander E.; Itskov, Mikhail // Journal of Materials Science;Nov2007, Vol. 42 Issue 21, p8853 

    In this paper a generalized anisotropic hyperelastic constitutive model for fiber-reinforced materials is proposed. Collagen fiber alignment in biological tissues is taken into account by means of structural tensors, where orthotropic and transversely isotropic material symmetries appear as...

  • Insulin-like growth factor 1 improves the efficacy of mesenchymal stem cells transplantation in a rat model of myocardial infarction. Jun Guo; Guosheng Lin; Cuiyu Bao; Zhimin Hu; Honggang Chu; Mingyan Hu // Journal of Biomedical Science;Jan2008, Vol. 15 Issue 1, p89 

    Background Previous study demonstrated the improvement of cardiac function was proportional to the number of cells implanted. Therefore, increasing cell survival in the infarcted myocardium might contribute to the improvement of the functional benefit of cell transplantation. Methods and results...

  • Hepatic morphological alterations, glycogen content and cytochrome P450 activities in rats treated chronically with Nω-nitro-L-arginine methyl ester (L-NAME). Tarsitano, Christiane Aprecida Badin; Paffaro, Valdemar A.; Pauli, José Rodrigo; Silva, Gustavo Henrique da; Saad, Mario J.; Salgado, Ione; Cruz-Höfling, Maria Alice da; Hyslop, Stephen // Cell & Tissue Research;Jun2007, Vol. 329 Issue 1, p45 

    Chronic treatment of rats with Nω-nitro-L-arginine methyl ester (L-NAME), an inhibitor of nitric oxide (NO) biosynthesis, results in hypertension mediated partly by enhanced angiotensin-I-converting enzyme (ACE) activity. We examined the influence of L-NAME on rat liver morphology, on...

  • Clusterin facilitates in vivo clearance of extracellular misfolded proteins. Wyatt, Amy; Yerbury, Justin; Berghofer, Paula; Greguric, Ivan; Katsifis, Andrew; Dobson, Christopher; Wilson, Mark // Cellular & Molecular Life Sciences;Dec2011, Vol. 68 Issue 23, p3919 

    The extracellular deposition of misfolded proteins is a characteristic of many debilitating age-related disorders. However, little is known about the specific mechanisms that act to suppress this process in vivo. Clusterin (CLU) is an extracellular chaperone that forms stable and soluble...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics