TITLE

Metody molekularne stosowane w identyfikacji grzybów z rodzaju Candida

AUTHOR(S)
Mecler, Izabela; Nawrot, Urszula
PUB. DATE
June 2008
SOURCE
Medical Mycology / Mikologia;2008, Vol. 15 Issue 2, p99
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The increase in percentage of infections due to Candida non-albicans, which often are difficult or no possible to identify by classical method, enhanced interest in developing of new, molecular-based methods of identifications. In this study we showed the possibilities and limitations of some molecular techniques, including PCR with specific and universal primers, analysis of the length of PCR product, restriction fragment length analysis, hybridization with specific probes, and DNA sequence analysis. DNA targets, e.g. rybosomal DNA, or mitochondria DNA, which are playing the role of molecular markers in construction the identification tests for Candida are also described.
ACCESSION #
37325370

 

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