Metody molekularne stosowane w identyfikacji grzybów z rodzaju Candida

Mecler, Izabela; Nawrot, Urszula
June 2008
Medical Mycology / Mikologia;2008, Vol. 15 Issue 2, p99
Academic Journal
The increase in percentage of infections due to Candida non-albicans, which often are difficult or no possible to identify by classical method, enhanced interest in developing of new, molecular-based methods of identifications. In this study we showed the possibilities and limitations of some molecular techniques, including PCR with specific and universal primers, analysis of the length of PCR product, restriction fragment length analysis, hybridization with specific probes, and DNA sequence analysis. DNA targets, e.g. rybosomal DNA, or mitochondria DNA, which are playing the role of molecular markers in construction the identification tests for Candida are also described.


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