Purified chickpea or lentil proteins impair VLDL metabolism and lipoprotein lipase activity in epididymal fat, but not in muscle, compared to casein, in growing rats

Boualga, Ahmed; Prost, Josiane; Taleb-Senouci, Douja; Krouf, Djamil; Kharoubi, Omar; Lamri-Senhadji, Myriem; Belleville, Jacques; Bouchenak, Malika
April 2009
European Journal of Nutrition;Apr2009, Vol. 48 Issue 3, p162
Academic Journal
It is well known that the legume proteins have a lowering effect on plasma cholesterol and triacylglycerols (TG) concentrations compared to animal proteins. The protein itself, as well as non-protein constituents, naturally present in legumes may be implicated. The effects of various dietary purified legumes proteins compared to casein, were determined on plasma TG level, VLDL concentration and composition. Moreover, lipoprotein lipase (LPL) activity in epididymal fat, gastrocnemius and heart was investigated to evaluate in these tissues their capacity to release free fatty acids from their TG substrate and the liver capacity to stock the TG. Weaning male Wistar rats were fed ad libitum one of the following diets: 200 g/kg diet of purified proteins of lentil (L), or chickpea (CP) or casein (CAS). At day 28, VLDL were isolated from plasma sample by a single ultracentrifugation flotation. Hepatic lipase and LPL activity in epididymal fat, gastrocnemius and heart were measured by using glycerol tri [9–10(n)-3H] oleate emulsion as substrate. Compared with CAS diet, the CP and L protein diets exhibited similar cholesterolemia, but lower triglyceridemia (1.9-fold and 2.5-fold) and VLDL particle number, as measured by their reduced contents of TG and apolipoproteins. CP and L protein diets reduced liver TG and cholesterol by 31 and 45%, respectively compared to CAS diet. Furthermore, LPL activity in adipose tissue of rats fed CP or L was 1.6-fold lower than that of rats fed CAS. There was no significant difference in heart and gastrocnemius LPL activities with the three proteins. In contrast, hepatic lipase activity was higher in rats fed CP and L diets. The low food efficiency ratio of purified CP and L proteins related to CAS is associated with decreased plasma VLDL and adipose tissue LPL activity. The low liver TG concomitant with reduced TG and apolipoproteins contents of VLDL confirm that hypotriglyceridemia is essentially due to impaired synthesis, exportation and transport of TG by VLDL which prevent lipid storage in adipose tissue.


Related Articles

  • Soy: The secret to radiant skin.  // South Asian Post;3/1/2012, p12 

    The article presents information on the health benefits of soy and legume which improve bone health and skin, reduce cholesterol and relieve menopausal symptoms.

  • POWER TRIO. STOPPANI, JIM // Joe Weider's Muscle & Fitness;May2011, Vol. 72 Issue 5, p62 

    The article presents information on the nutritional facts related to protein sources, particularly for individuals doing physical training. It is stated that one needs to ingest more protein, so as to grow more muscles, because muscle is made up of protein. Various sources include whey protein,...

  • Better Together. RADU, RAZVAN // Muscle & Fitness;Dec2015, Vol. 76 Issue 11, p142 

    The article reports on the effectiveness of protein blends in building muscles. The different types of protein are examined, along with their health benefits, including whey, casein, and soy. A study which reveals the greater anabolic response of protein blends than a pure whey protein is also...

  • Ask the RD?  // IDEA Fitness Journal;Jun2012, Vol. 9 Issue 6, p67 

    The article provides an answer to a question of what type of protein should be consumed after workout.

  • 'First of its kind' study into muscle-building protein.  // Food Manufacture;Jun2012, Vol. 87 Issue 6, p19 

    The article focuses on the findings of a clinical study related to muscle-building protein, led by Blake Rasmussen, interim chairman of the Department of Nutrition & Metabolism at the University of Texas Medical Branch. The study titled "Effect of protein blend versus whey protein ingestion on...

  • ASK THE EXPERT. PROTEIN POWDERS. Amidor, Toby // Today's Dietitian;Mar2012, Vol. 14 Issue 3, p10 

    The article provides an answer to the question related to the types of recommended protein powders.

  • Reader's Poll.  // Joe Weider's Muscle & Fitness;Aug2009, Vol. 70 Issue 8, p45 

    The article focuses on the findings of a public opinion poll related to the consumption of protein supplements before sleeping and the response of the magazine's editor Jim Stoppani to it. The results show that 30% of the respondents never take the supplements, 18% consume the supplements every...

  • PeptoPro could help elderly or obese.  // Food Manufacture;May2005, Vol. 80 Issue 5, p18 

    This article reports that the company DSM says its high protein ingredient PeptoPro can benefit a wider audience than simply athletes and exercise fanatics. It could be used to create healthier soft drinks for kids and for recovery products aimed at elderly hospital patients. The patented drink,...

  • Nutritional Factors in the Etiology of Brain Tumors Potential Role of Nitrosamines, Fat, and Cholesterol. Kaplan, Sara; Novikov, llya; Modan, Baruch // American Journal of Epidemiology;1997, Vol. 146 Issue 10, p832 

    Several possible risk factors for brain tumors have been suggested in the past, including N-nitroso compounds, but with the exception of ionizing radiation, none has been consistently confirmed. The present study was aimed at assessing the influence of nutritional factors, including N-nitroso...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics