TITLE

Peripheral blood leukocyte distribution and body mass index are associated with the methylation pattern of the androgen receptor promoter

AUTHOR(S)
Sofia Movérare-Skrtic; Dan Mellström; Liesbeth Vandenput; Mathias Ehrich; Claes Ohlsson
PUB. DATE
April 2009
SOURCE
Endocrine (1355008X);Apr2009, Vol. 35 Issue 2, p204
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Abstract  Methylation of CpG sites in the promoter region can affect gene transcription. DNA derived from peripheral blood leukocytes (PBL) from the well-characterized clinical cohorts might be useful to study the influence of environmental factors on DNA methylation. However, these studies could be confounded by the heterogeneous nature of PBL. The aims of this study were to determine the impact of PBL distribution on methylation status of the androgen receptor (AR) promoter, and determine the associations between PBL distribution-adjusted methylation status of the AR promoter and AR-related phenotypes. PBL differential count analyses were performed at the time of blood sampling for DNA preparation in 170 elderly men. The DNA was bisulfite treated, and the methylation status of five CpG units in the AR promoter was analyzed using a high-throughput technique based on MALDI-TOF mass spectrometry. The degree of methylation of all the five investigated CpG units was strongly positively associated with the percent of lymphocytes in the PBL (r s = 0.17–0.49, P r s = 0.24) and other measures reflecting fat mass in elderly men. In conclusion, adjustment for PBL distribution needs to be done to be able to use DNA from whole blood for methylation analysis of the AR promoter and most likely also when investigating other promoters.
ACCESSION #
37142373

 

Related Articles

  • Distinct and Histone-Specific Modifications Mediate Positive versus Negative Transcriptional Regulation of TSHα Promoter. Dongqing Wang; Xianmin Xia; Weiss, Roy E.; Refetoff, Samuel; Yen, Paul M. // PLoS ONE;2010, Vol. 5 Issue 3, p1 

    Background: Hormonally-regulated histone modifications that govern positive versus negative transcription of target genes are poorly characterized despite their importance for normal and pathological endocrine function. There have been only a few studies examining chromatin modifications on...

  • An alternative pathway for transcription initiation involving TFII-I. Roy, Ananda L.; Malik, Sohail // Nature;9/23/1993, Vol. 365 Issue 6444, p355 

    Describes a pathway for transcription initiation requiring an intact initiator element (Inr) and the Inr-binding factor TFII-I. Minimal promoter elements for initiation by RNA polymerase II; Support for functionally distinct pathways subject to differential regulation by specific activators or...

  • Age-Related Changes in the Global DNA Methylation Profile of Leukocytes Are Linked to Nutrition but Are Not Associated with the MTHFR C677T Genotype or to Functional Capacities. Gomes, Marcus V. M.; Toffoli, Leandro V.; Arruda, Douglas W.; Soldera, Larissa M.; Pelosi, Gislaine G.; Neves-Souza, Rejane D.; Freitas, Eliane R.; Castro, Denilson T.; Marquez, Audrey S. // PLoS ONE;Dec2012, Vol. 7 Issue 12, p1 

    Global DNA methylation of peripheral blood leukocytes has been recently proposed as a potential biomarker for disease risk. However, the amplitude of the changes in DNA methylation associated with normal aging and the impacts of environmental changes on this variation are still unclear. In this...

  • DNA Methylation Profiling of the Fibrinogen Gene Landscape in Human Cells and during Mouse and Zebrafish Development. Vorjohann, Silja; Pitetti, Jean-Luc; Nef, Serge; Gonelle-Gispert, Carmen; Buhler, Leo; Fish, Richard J.; Neerman-Arbez, Marguerite // PLoS ONE;Aug2013, Vol. 8 Issue 8, p1 

    The fibrinogen genes FGA, FGB and FGG show coordinated expression in hepatocytes. Understanding the underlying transcriptional regulation may elucidate how their tissue-specific expression is maintained and explain the high variability in fibrinogen blood levels. DNA methylation of CpG-poor gene...

  • The methylation of the LEPR/LEPROT genotype at the promoter and body regions influence concentrations of leptin in girls and BMI at age 18 years if their mother smoked during pregnancy. Yousefi, Mitra; Karmaus, Wilfried; Zhang, Hongmei; Ewart, Susan; Arshad, Hasan; Holloway, John W. // International Journal of Molecular Epidemiology & Genetics;2013, Vol. 4 Issue 2, p86 

    To determine whether DNA methylation (DNA-M) of the leptin receptor genotype (LEPR/LEPROT) links gestational smoking and leptin serum levels and BMI later in life, we focused on female offspring, 18 years of age, from the Isle of Wight Birth Cohort (IOWBC). Leptin binds to the leptin receptor...

  • Targeted ribose methylation of RNA in vivo directed by tailored antisense RNA guides. Cavaille, Jerome; Nicoloso, Monique // Nature;10/24/1996, Vol. 383 Issue 6602, p732 

    Demonstrates that altering the sequence of the small nucleolar RNAs (snoRNA) is sufficient to change the specificity of methylation. The identification of the structural features required for methylation; The guide and substrate RNA required; The designing of an exogenous transcript, devoid of...

  • Too much sex is bad for males. Swain, Amanda; Lovell-Badge, Robin // Nature;11/12/1998, Vol. 396 Issue 6707, p115 

    Discusses research which identified an X-linked gene that encodes a protein related to nuclear hormone receptors in Caenorhabditis elegans. Research by Carmi and colleagues in this issue; Protein SEX-1 repressing the transcription of xol-1; Downregulation of XOL-1; Supporting research; Use of a...

  • The nuclear hormone receptor SEX-1 is an X-chromosome signal that determines nematode sex. Carmi, Ilil; Kopczynski, Jennifer B.; Meyer, Barbara J. // Nature;11/12/1998, Vol. 396 Issue 6707, p168 

    Presents research which identified a nuclear-hormone-receptor homologue, SEX-1, that regulates the transcription of xol-1 in Caenorhabditis elegans. Molecular aspects of the X-chromosome counting mechanism; Role of X signal elements; Repression of xol-1; SEX-1 protein acting on xol-1 directly;...

  • A Prospective Study of LINE-1DNA Methylation and Development of Adiposity in School-Age Children. Perng, Wei; Mora-Plazas, Mercedes; Marín, Constanza; Rozek, Laura S.; Baylin, Ana; Villamor, Eduardo // PLoS ONE;Apr2013, Vol. 8 Issue 4, p1 

    Background: Repetitive element DNA methylation is related to prominent obesity-related chronic diseases including cancer and cardiovascular disease; yet, little is known of its relation with weight status. We examined associations of LINE-1 DNA methylation with changes in adiposity and linear...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics