TITLE

DETERMINACION DE ANTICUERPOS ANTICELULAS BETA MEDIANTE UN METODO CUALITATIVO POR ENZIMA INMUNOENSAYO (ELISA). COMPARACION CON TECNICA DE INMUNOFLUORESCENCIA INDIRECTA (IFI)

AUTHOR(S)
Escalona O., Manuel; Acosta B., Ana María
PUB. DATE
December 2008
SOURCE
Revista Chilena de Tecnología Médica;2008, Vol. 28 Issue 2, p1441
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Diabetes mellitus type 1 (DM1) is an autoimmune disease characterized by the presence of antibodies on the surface of pancreatic beta cell, these antibodies can also be detected in the preclinic stage before onset of the disease. Among these antibodies are islet cells cytoplasmic antibodies (ICA) who have been directly associated with the onset of type 1 diabetes mellitus. The reference method is the indirect immunofluorescence (IFI) and by today, there is a qualitative enzyme-linked immunosorbent assay (ELISA) for the detection of ICA. The aim of this study was to determine in a group of 167 subjects ICA by ELISA and compare their results with IFI. RESULTS: We found 10 positive for ICA with IFI and 9 by Elisa, 70% of ICA positive IFI were among individuals younger. Elisa was matched only 2 patients. CONCLUSIONS: Whereas the ICA have been primarily used for identifying individuals at risk for developing DM1 and that the presence of high-titer ICA at diagnosis is associated with a rapid decrease in C-peptide levels seems appropriate to determine the ICA through IFI, despite laborious nature of the technical and difficult to process large volumes of samples.
ACCESSION #
36852781

 

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