Effects of Fluoride-Modified Titanium Surfaces on Osteoblast Proliferation and Gene Expression

Isa, Zakiah M.; Schneider, Galen B.; Zaharias, Rebecca; Seabold, Denise; Stanford, Clark M.
March 2006
International Journal of Oral & Maxillofacial Implants;Mar/Apr2006, Vol. 21 Issue 2, p203
Academic Journal
Purpose: The objective of this study was to test the hypothesis that fluoride-modified titanium surfaces would enhance osteoblast differentiation. Osteoblast growth on a moderately rough etched fluoride-modified titanium surface (alteration in cellular differentiation) was compared to osteoblast growth on the same surface grit-blasted with titanium dioxide. The potential role of nanometer-level alterations on cell shape and subsequent differentiation was then compared. Materials and Methods: Human embryonic palatal mesenchymal (HEPM) cultures were incubated on the respective surfaces for 1, 3, and 7 days, followed by analysis for cell proliferation, alkaline phosphatase (ALP) -specific activity, and mRNA steady-state expression for bone-related genes (ALP, type I collagen, osteocalcin, bone sialo protein [BSP] II, Cbfa1, and osterix) by real-time polymerase chain reaction (PCR). Results: The different surfaces did not alter the mRNA expression for ALP, type I collagen, osterix, osteocalcin, or BSP II. However, Cbfa1 expression on the fluoride-modified titanium surface was significantly higher (P < .001) at 1 week. The number of cells on this surface was 20% lower than the number of cells on the surface TiO2-blasted with 25-μm particles but not significantly different from the number of cells on the surface TiO2-blasted with 125-am particles. Cells grown on all the titanium surfaces expressed similar levels of ALP activity. Conclusions: The results indicated that a fluoride-modified surface topography, in synergy with surface roughness, may have a greater influence on the level of expression of Cbfa1 (a key regulator for osteogenesis) than the unmodified titanium surfaces studied.


Related Articles

  • The Importance of Implant Surface Characteristics in the Replacement of Failed Implants. Alsaadi, Ghada; Quirynen, Marc; van Steenberghe, Daniel // International Journal of Oral & Maxillofacial Implants;Mar/Apr2006, Vol. 21 Issue 2, p270 

    Purpose: The purpose of the study was to compare the failure rates of implants with either a machined surface or a TiUnite surface used to replace failing implants. Materials and Methods: The files of 578 patients, ie, of all patients who were treated at the Department of Periodontology of the...

  • Expression of transcription factor E2F1 induces quiescent cells to enter S phase. Johnson, David G.; Schwarz, James K. // Nature;9/23/1993, Vol. 365 Issue 6444, p349 

    Provides evidence showing that E2F transcription factors are involved in cellular proliferation control. Effect of overexpression of the E2F1 complementary DNA (cDNA) on DNA synthesis in cells; S-phase entry in quiescent cells after microinjection of E2F1 cDNA; Coincidence with capacity to...

  • Apatite/Amelogenin Coating on Titanium Promotes Osteogenic Gene Expression. Du, C.; Schneider, G. B.; Zaharias2, R.; Abbott, C.; Seabold, D.; Stanford, C.; Moradian-Oldak, J. // Journal of Dental Research;Nov2005, Vol. 84 Issue 11, p1070 

    Osteoblast differentiation and extracellular matrix production are pivotal processes for implant osseointegration or bone tissue engineering. We hypothesized that a biomimetic coating on titanium surfaces, consisting of apatite and amelogenin, would promote such processes. Human Embryonic...

  • Surface-Etching Enhances Titanium Implant Osseointegration in Newly Formed (rhBMP-2-Induced) and Native Bone. Qahash, Mohammed; Hardwick, W. Ross; Rohrer, Michael D.; Wozney, John M.; Wikesjö, Ulf M. E. // International Journal of Oral & Maxillofacial Implants;May/Jun2007, Vol. 22 Issue 3, p472 

    Purpose: The influence of surface modifications on osseointegration in newly formed bone is not well established. The purpose of this study was to compare osseointegration at acid-etched versus turned implants in newly formed and native bone. Methods: Supra-alveolar peri-implant defects were...

  • Histomorphometric Study of Ion Implantation and Diamond-like Carbon as Dental Implant Surface Treatments in Beagle Dogs. De Maeztu, Miguel A.; Braceras, Iñigo; Iñaki^Alava, J.; Sánchez-Garcés, M. Angeles; Gay-Escoda, Cosme // International Journal of Oral & Maxillofacial Implants;Mar/Apr2007, Vol. 22 Issue 2, p273 

    Purpose: Improvements in the bone-implant interface can provide clinical benefits, such as increasing the amount of bone in contact with the implant and shortening the time required to achieve sufficient bone appositioning to allow early prosthetic loading. The present study describes the...

  • Topographic analysis of the surface of commercially pure titanium implants. Study using scanning electron microscopy. Da PAIXÃO, Amanda Hora; ROCHA, José Rodrigo Mega; BOTTO, Bruno; Oliveira MIRANDA, Dario Augusto; Santana SARDINHA, Sandra de Cássia // Dental Press Implantology;Apr-Jun2013, Vol. 7 Issue 2, p49 

    Objective: This study aims at carrying out a descriptive comparative analysis of four types of surfaces of commercially pure titanium implants by means of scanning electron microscopy (SEM). Material and Methods: Four implants of different commercial brands were used, as follows: Conexão -...

  • Shrinkage control: regulation of insulin-mediated growth by FOXO transcription factors.  // Journal of Biology;2003, Vol. 2, p18 

    The insulin signaling pathway regulates organismal growth in response to nutrient conditions by controlling a range of metabolic and biosynthetic processes. Recent studies in Drosophila have shown how transcriptional responses to reduced insulin and nutrient levels can act to inhibit growth.

  • Controlling how many cells make a fly.  // Journal of Biology;2003, Vol. 2, p16 

    The article discusses a report within the issue regarding research by Ernst Hafen and colleagues of the University of Zürich, Switzerland that revealed the Forkhead-family transcription factor FOXO in the fruit fly Drosophila melanogaster is a crucial mediator of the S6 kinase branch of the...

  • Replication stress checkpoint signaling controls tRNA gene transcription. Nguyen, Vesna C.; Clelland, Brett W.; Hockman, Darren J.; Kujat-Choy, Sonya L.; Mewhort, Holly E.; Schultz, Michael C. // Nature Structural & Molecular Biology;Aug2010, Vol. 17 Issue 8, p976 

    In budding yeast, the transcriptional machinery at tRNA genes naturally interferes with replication in a way that can promote chromosome breakage. Here we show that a signaling module composed of core components of the replication stress checkpoint pathway represses this fork-pausing machinery...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics