TITLE

Effects of Fluoride-Modified Titanium Surfaces on Osteoblast Proliferation and Gene Expression

AUTHOR(S)
Isa, Zakiah M.; Schneider, Galen B.; Zaharias, Rebecca; Seabold, Denise; Stanford, Clark M.
PUB. DATE
March 2006
SOURCE
International Journal of Oral & Maxillofacial Implants;Mar/Apr2006, Vol. 21 Issue 2, p203
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Purpose: The objective of this study was to test the hypothesis that fluoride-modified titanium surfaces would enhance osteoblast differentiation. Osteoblast growth on a moderately rough etched fluoride-modified titanium surface (alteration in cellular differentiation) was compared to osteoblast growth on the same surface grit-blasted with titanium dioxide. The potential role of nanometer-level alterations on cell shape and subsequent differentiation was then compared. Materials and Methods: Human embryonic palatal mesenchymal (HEPM) cultures were incubated on the respective surfaces for 1, 3, and 7 days, followed by analysis for cell proliferation, alkaline phosphatase (ALP) -specific activity, and mRNA steady-state expression for bone-related genes (ALP, type I collagen, osteocalcin, bone sialo protein [BSP] II, Cbfa1, and osterix) by real-time polymerase chain reaction (PCR). Results: The different surfaces did not alter the mRNA expression for ALP, type I collagen, osterix, osteocalcin, or BSP II. However, Cbfa1 expression on the fluoride-modified titanium surface was significantly higher (P < .001) at 1 week. The number of cells on this surface was 20% lower than the number of cells on the surface TiO2-blasted with 25-μm particles but not significantly different from the number of cells on the surface TiO2-blasted with 125-am particles. Cells grown on all the titanium surfaces expressed similar levels of ALP activity. Conclusions: The results indicated that a fluoride-modified surface topography, in synergy with surface roughness, may have a greater influence on the level of expression of Cbfa1 (a key regulator for osteogenesis) than the unmodified titanium surfaces studied.
ACCESSION #
36831329

 

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