Metabonomics studies of intact hepatic and renal cortical tissues from diabetic db/db mice using high-resolution magic-angle spinning 1H NMR spectroscopy

Jingjing Xu; Jun Zhang; Jiyang Dong; Shuhui Cai; Yang, James Y.; Zhong Chen
March 2009
Analytical & Bioanalytical Chemistry;Mar2009, Vol. 393 Issue 6/7, p1657
Academic Journal
A metabonomics approach based on high-resolution magic-angle spinning (HRMAS) 1H NMR spectroscopy was applied to investigate the metabolite composition in intact hepatic tissues and renal cortical tissues from db/db mice of 8 weeks old, an animal model of type 2 diabetes mellitus. Compared to the control group, the hepatic tissues of diabetic mice have elevated levels of triglyceride and bile acid and declined levels of trimethylamine- N-oxide, phosphocholine, glycerophosphocholine, and choline. The biochemical changes are less obvious in renal cortical tissues of diabetic mice. The WET_CPMG pulse sequence was selected for our metabonomics study after the quality and reproducibility of the spectra obtained from the NOEPR, NOEPR_CPMG, and WET_CPMG pulse sequences were analyzed together with principal component analysis. The influence of line-broadening factor of exponential window function for spectral manipulation on class separation was paid attention to for the first time, and an optimal value was obtained under our experimental conditions. These studies show the efficiency of HRMAS 1H NMR spectroscopy for tissue metabonomics study in combination with multivariate statistical analysis, which may help to explore the etiological factor of diabetes mellitus from a new perspective. [Figure not available: see fulltext.]


Related Articles

  • Differences in Metabolites in Pain-Processing Brain Regions in Patients With Diabetes and Painful Neuropathy. Sorensen, Lea; Siddall, Philip J.; Trenell, Michael I.; Yue, Dennis K. // Diabetes Care;May2008, Vol. 31 Issue 5, p980 

    OBJECTIVE -- Magnetic resonance spectroscopy (MRS) (specifically, ¹H-MRS) has been used to show changes in the brain following peripheral nerve injury in subjects without diabetes. This study used ¹H-MRS to examine the brain in subjects with or without painful diabetic neuropathy. RESEARCH...

  • biospectroscopy.  // Taber's Cyclopedic Medical Dictionary (2009);2009, Issue 21, p267 

    A definition of the term "biospectroscopy," which refers to the examination of tissue by use of a spectroscope, is presented.

  • Spectroscopy and imaging with diffusing light. Yodh, Arjun; Chance, Britton // Physics Today;Mar95, Vol. 48 Issue 3, p34 

    Investigates the physical basis for tissue characterization in turbid media using diffusing light fields. Contrast between spectroscopy and imaging; Functional imaging and object localization; In vitro absorption spectra; Interaction of light and tissue; Distortion of light energy density as...

  • Tissue Metallothionein Concentrations in Mice and Humans with Hyperglycemia. Yuh-Min Song // Biological Trace Element Research;Mar2009, Vol. 127 Issue 3, p251 

    Abstract  Besides participating in tissue zinc homeostasis and protecting against heavy metal toxicities, metallothionein (MT) is known as an antioxidant. Increased MT activity can ameliorate diabetic hyperglycemia, and subjects with less MT synthesis are more prone to diabetic...

  • Intra-operative optical diagnostics with vibrational spectroscopy. Stelling, Allison; Salzer, Reiner; Kirsch, Matthias; Sobottka, Stephan B.; Geiger, Kathrin; Koch, Edmund; Schackert, Gabriele; Steiner, Gerald // Analytical & Bioanalytical Chemistry;Oct2011, Vol. 400 Issue 9, p2745 

    Established methods for characterization of tissue and diagnostics, for example histochemistry, magnetic resonance imaging (MRI), X-ray tomography, or positron emission tomography (PET), are mostly not suitable for intra-operative use. However, there is a clear need for an intra-operative...

  • Noninvasive methods of measuring oxygen saturation in venous blood. Yesman, S.; Mamilov, S.; Asimov, M.; Gisbreht, A. // Journal of Applied Spectroscopy;Jul2011, Vol. 78 Issue 3, p406 

    The feasibility of measuring oxygen saturation in venous blood both by artificial modulation of the volume of tissue filled with blood and by determining the respiratory rhythm from a Fourier spectrum is demonstrated. Both methods have advantages and limitations. The results obtained by the two...

  • Increased metabolite concentration in migraine rat model by proton MR spectroscopy in vivo and ex vivo. Zhe Ma; Sheng-jun Wang; Chuan-fu Li; Xiang-xing Ma; Tao Gu // Neurological Sciences;Oct2008, Vol. 29 Issue 5, p337 

    To investigate brain metabolite change in rat migraine model by proton magnetic resonance spectroscopy (1H-MRS) in vivo and ex vivo. 18 Adult SD rats were enrolled in the study. After nitroglycerin administration, the migraine rat model was established according to ethology evaluation. 1H-MRS...

  • Protective effect of extracellular protein metabolite of Luteococcus japonicus subsp. casei on cells subjected to heating and UV irradiation. Vorob’eva, L.; Khodzhaev, E.; Ponomarev, G. // Applied Biochemistry & Microbiology;Jan2008, Vol. 44 Issue 1, p38 

    Extracellular protein metabolite isolated from the culture liquid of Luteococcus japonicus subsp. casei had reactivating and protective effects on UV-irradiated and heated cells. The extracellular metabolite, produced by cells in the logarithmic growth phase, was present in culture liquid in...

  • Convolinine, a new alkaloid from Convolvulus subhirsutus of Uzbekistan flora. Gapparov, A. M.; Aripova, S. F.; Tashkhodzhaev, B.; Levkovich, M. G.; Aripov, O. // Chemistry of Natural Compounds;Sep2010, Vol. 46 Issue 4, p590 

    Several alkaloids of known structure and the new base convolinine were isolated from total alkaloids from the aerial part of Convolvulis subhirsutus growing in Uzbekistan. It has been established that convolinine is an N-hydroxyethyl derivative of convolvine and has the structure (±)-...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics