TITLE

Combined Porin Loss and Extended Spectrum β-Lactamase Production is Associated with an Increasing Imipenem Minimal Inhibitory Concentration in Clinical Klebsiella pneumoniae Strains

AUTHOR(S)
Duo Yang; Yu Guo; Zheng Zhang
PUB. DATE
April 2009
SOURCE
Current Microbiology;Apr2009, Vol. 58 Issue 4, p366
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
For this study, 150 clinical isolates of Klebsiella pneumoniae were collected from one hospital in Beijing, China, and assayed for minimal inhibitory concentration (MIC) of imipenem. To elucidate the mechanisms responsible for imipenem MIC variation among extended-spectrum β-lactamase (ESBL)-positive and -negative K. pneumoniae strains, a variety of β-lactamase genes ( blaTEM, blaCTX-M, blaSHV, and blaOXA) were screened by polymerase chain reaction (PCR). The outer membrane profile and expression of related genes ( ompK35 and ompK36) then were analyzed and evaluated, respectively. None of the tested isolates were clinically resistant to imipenem, but the range of MICs among ESBL-positive and -negative strains was significantly different. Deficiency in the expression of outer membrane proteins (OmpK35,36) was observed in some of both ESBL-positive(17.6%) and -negative strains (10.9%), but only the ESBL-positive strains depressed by the expression of ompK35/36 had an increased MIC of imipenem (≥0.5 mg/l). These results confirmed that the combination of SHV-1, CTX-M-3, CTX-M-14, TEM-1, or OXA-11 production and reduced expression of ompK35/36 may not result in clinical resistance to imipenem but does correlate with increasing imipenem MIC.
ACCESSION #
36778833

 

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