TITLE

Clinically Relevant Cell Sources for TMJ Disc Engineering

AUTHOR(S)
Johns, D. E.; Wong, M. E.; Athanasiou, K. A.
PUB. DATE
June 2008
SOURCE
Journal of Dental Research;Jun2008, Vol. 87 Issue 6, p548
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Tissue-engineering of the temporomandibular joint (TMJ) disc aims to provide patients with TMJ disorders an option to replace diseased tissue with autologous, functional tissue. This study examined clinically relevant cell sources by comparing costal chondrocytes, dermal fibroblasts, a mixture of the two, and TMJ disc cells in a scaffoldless tissue-engineering approach. It was hypothesized that all constructs would produce matrix relevant to the TMJ disc, but the mixture constructs were expected to appear most like the TMJ disc constructs. Costal chondrocyte and mixture constructs were morphologically and biochemically superior to the TMJ disc and dermal fibroblast constructs, and their compressive properties were not significantly different. Costal chondrocyte constructs produced almost 40 times more collagen and 800 times more glycosaminoglycans than did TMJ constructs. This study demonstrates the ability of costal chondrocytes to produce extracellular matrix that may function in a TMJ disc replacement.
ACCESSION #
36634989

 

Related Articles

  • Morphologic, Microscopic, and Immunohistochemical Investigations Into the Function of the Primate TMJ Disc. Mills, David K.; Fiandaca, Dante J.; Scapino, Robert P. // Journal of Orofacial Pain;Spring1994, Vol. 8 Issue 2, p136 

    The distribution and arrangement of extracellular matrix proteins were examined in the primate temporomandibular joint disc and posterior attachment using a combination of light microscopic, immunohistochemical, and biochemical techniques. The band areas of the disc contain a complex collagenous...

  • Opinion Statement of the Effect of Mechanical Stress on Cartilage Tissue Engineering. Ando, Kosei; Mimura, Tomohiro; Matsusue, Yoshitaka; Mori, Kanji // Open Bone Journal;2010, Vol. 2, p32 

    Articular cartilage is characterized by its poor capacity for self-repair. Once articular cartilage is injured and defected, it cannot be spontaneously repaired and finally develops osteoarthritis (OA). OA is a major leading cause of severe activity limitations and disability, resulting in...

  • Effects of exogenous glycosaminoglycans on human chondrocytes cultivated on type II collagen scaffolds. Chun-Hsien Wu; Chih-Sheng Ko; Jia-Wen Huang; Hsin-Jie Huang; I-Ming Chu // Journal of Materials Science: Materials in Medicine;Feb2010, Vol. 21 Issue 2, p725 

    Cartilage extracellular matrix (ECM) is composed primarily of type II collagen (COL II) and large, networks of proteoglycans (PGs) that contain glycosaminoglycans such as hyaluronic acid (HA) and chondroitin sulfate (CS). Since cartilage shows little tendency for self-repair, injuries are kept...

  • In vitro construction of scaffold-free cylindrical cartilage using cell sheet-based tissue engineering. Tani, Gakuto; Usui, Noriaki; Kamiyama, Masafumi; Oue, Takaharu; Fukuzawa, Masahiro // Pediatric Surgery International;Feb2010, Vol. 26 Issue 2, p179 

    Purpose: Tissue-engineered cartilage may offer a solution for the treatment of serious airway disease. This study developed a novel procedure to fabricate a scaffold-free cylindrical cartilage under in vitro conditions, while also evaluating the effect of a dynamic culture on the...

  • Redifferentiation of dedifferentiated human chondrocytes in high-density cultures. Schulze-Tanzil, G.; de Souza, P.; Villegas Castrejon, H.; John, T.; Merker, H.-J.; Scheid, A.; Shakibaei, M. // Cell & Tissue Research;Jun2002, Vol. 308 Issue 3, p371 

    High-density cultures are widely used as an in vitro model for studies of embryonic cartilage formation. In the present study we investigated the suitability of high-density cultures for the redifferentiation of dedifferentiated chondrocytes. When primary human chondrocytes were cultured in...

  • The potential of pulsed low intensity ultrasound to stimulate chondrocytes matrix synthesis in agarose and monolayer cultures. Vaughan, Natalie; Grainger, James; Bader, Dan; Knight, Martin // Medical & Biological Engineering & Computing;Dec2010, Vol. 48 Issue 12, p1215 

    Pulsed low intensity ultrasound (PLIUS) has been used successfully for bone fracture repair and has therefore been suggested for cartilage regeneration. However, previous in vitro studies with chondrocytes show conflicting results as to the effect of PLIUS on the elaboration of extracellular...

  • Time-Dependent Processes in Stem Cell-Based Tissue Engineering of Articular Cartilage. Gadjanski, Ivana; Spiller, Kara; Vunjak-Novakovic, Gordana // Stem Cell Reviews & Reports;Sep2012, Vol. 8 Issue 3, p863 

    Articular cartilage (AC), situated in diarthrodial joints at the end of the long bones, is composed of a single cell type (chondrocytes) embedded in dense extracellular matrix comprised of collagens and proteoglycans. AC is avascular and alymphatic and is not innervated. At first glance, such a...

  • Diphenylhydantoin Affects Glycosaminoglycans and Collagen Production by Human Fibroblasts from Cleft Palate Patients. Bosi, G.; Evangelisti, R.; Valeno, V.; Carinci, F.; Pezzetti, F.; Calastrini, C.; Bodo, M.; Carinci, P. // Journal of Dental Research;Aug1998, Vol. 77 Issue 8, p1613 

    During embryonic development, the proper production of extracellular matrix molecules mediates morphogenetic processes involved in palatogenesis. In the present study, we investigated whether any differences exist in glycosaminoglycan (GAG) and collagen synthesis between palate fibroblasts from...

  • Regeneration of static-load-degenerated articular cartilage extracellular matrix by vitamin C supplementation. Sharma, Garima; Saxena, R. K.; Mishra, Prashant // Cell & Tissue Research;Oct2008, Vol. 334 Issue 1, p111 

    The effect of a physiological dose of vitamin C (100 μg/ml) on goat articular cartilage chondrocytes cultured in an alginate matrix and subjected to static pressurization of 2.4 MPa was investigated. Biochemical analyses of DNA, glycosaminoglycan (GAG), collagen and protease activity were...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics