TITLE

Telomerase activity (TMA) in tumour and peritumoural tissues in a rat liver cancer model

AUTHOR(S)
Huo-Jun Zhang; Ji-Jin Yang; Jian-Ming Tian; Pei-Jun Wang; Cheng-Wei Shao; Chang-Jing Zuo; Shun-Min Zhang; Gupta, Sanjay
PUB. DATE
February 2009
SOURCE
Clinical & Investigative Medicine;Feb2009, Vol. 32 Issue 1, pE8
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Purpose: To study the levels of telomerase activity (TMA) in tumour and peritumoural tissues in a liver cancer model in rats, and to study the change in TMA expression over time. Methods: Using the telomeric repeated amplification protocol (TRAP), TMA was measured in tumour tissue, peritumoural tissue and normal liver tissue of Walker-256 tumour-bearing rats at 4, 6 and 8 days after tumour implantation. Results: TMA at day 4, 6 and 8 was 0.767±0.117, 0.768±0.118 and 0.774±0.111 in tumour tissue, 0.389±0.263, 0.492±0.253 and 0.584±0.239 in peritumoural tissue, and 0.231±0.022, 0.229±0.022 and 0.233±0.021 in normal liver tissue, respectively. TMA in tumour tissue was higher than that in peri-tumour and normal liver tissues at all time points of measurement (P<0.05). The TMA levels in tumour tissue and normal liver tissue did not show any change over time. TMA level in the peritumoural tissue increased with time; TMA level in animals sacrificed at day 8 was higher than that seen in animals sacrificed at day 4 (P<0.05). Conclusion: TMA in walker-256 tumour-bearing rats was higher than that in normal and peritumoural tissues. TMA level in the peritumoural tissue increased with time suggesting that TMA activation in peritumoural tissue may be an important factor promoting tumour growth.
ACCESSION #
36620031

 

Related Articles

  • Correlation between Telomerase Activity and DNA Ploidy in Gastric Cancer. Okusa, Yasushi; Shinomiya, Nariyoshi; Ichikura, Takashi; Mochizuki, Hidetaka // Oncology;1998, Vol. 55 Issue 3, p258 

    Telomerase has been reported to be activated in most immortal cells and human cancers. In the present study, we assessed the correlation between telomerase activity and cellular DNA ploidy level in gastric cancer. Telomerase activity was determined semiquantitatively using the telomeric repeat...

  • The Two Faces of Telomerase. Allsopp, Richard // Hawaii Medical Journal;Feb2005, Vol. 64 Issue 2, p50 

    Reviews the advantages and disadvantages of telomerase with regards to its potential therapeutic applications. Description of the physiological role of telomerase; Activation of telomerase in cancer cells; Role of telomerase in the survival of most types of cancer.

  • Oligomer-mediated modulation of hTERT alternative splicing induces telomerase inhibition and cell growth decline in human prostate cancer cells. C. Brambilla; M. Folini; P. Gandellini; L. Daprai; M. G. Daidone; N. Zaffaroni // Cellular & Molecular Life Sciences;Jul2004, Vol. 61 Issue 14, p1764 

    The expression of telomerase in human cells is strictly controlled by multiple mechanisms including transcription and alternative splicing of telomerase reverse transcriptase (hTERT). In this study, we demonstrated the possibility of modulating the hTERT splicing pattern in DU145 human prostate...

  • PML body meets telomere. Chung, Inn; Osterwald, Sarah; Deeg, Katharina I.; Rippe, Karsten // Nucleus (1949-1034);2012, Vol. 3 Issue 3, p263 

    The unlimited proliferation potential of cancer cells requires the maintenance of their telomeres. This is frequently accomplished by reactivation of telomerase. However, in a significant fraction of tumors an alternative lengthening of telomeres (ALT) mechanism is active. The molecular...

  • Keeping telomerase in its place. Maser, Richard S.; DePinho, Ronald A. // Nature Medicine;Sep2002, Vol. 8 Issue 9, p934 

    Focuses on a component of telomerase, TERT, that shuttles between nuclear compartments during the cell cycle. Upregulation of telomerase in human cancers through reactivation of TERT expression; Benefits of disruption of normal telomerase shuttling for cancer cells.

  • Up-Regulation of Telomerase in Primary Cultured Rat Hepatocytes1. Nozawa, Katsura; Kurumiya, Yasuhiro; Yamamoto, Akiko; Isobe, Yuka; Suzuki, Motoshi; Yoshida, Shonen // Journal of Biochemistry;1999, Vol. 126 Issue 2, p361 

    Telomerase is a unique reverse transcriptase involved in the maintenance of telomeric DNA, which is generally undetectable in normal human somatic cells. However, it has been found in organs of normal adult rodents including the liver. In order to elucidate relevant control mechanisms operating...

  • Telomerase Inhibition Targets Clonogenic Multiple Myeloma Cells through Telomere Length-Dependent and Independent Mechanisms. Brennan, Sarah K.; Qiuju Wang; Tressler, Robert; Harley, Calvin; Ning Go; Bassett, Ekaterina; Huff, Carol Ann; Jones, Richard J.; Matsui, William // PLoS ONE;2010, Vol. 5 Issue 9, p1 

    Background: Plasma cells constitute the majority of tumor cells in multiple myeloma (MM) but lack the potential for sustained clonogenic growth. In contrast, clonotypic B cells can engraft and recapitulate disease in immunodeficient mice suggesting they serve as the MM cancer stem cell (CSC)....

  • A highly selective telomerase inhibitor limiting human cancer cell proliferation. Damm, Klaus; Hemmann, Ulrike; Garin-chesa, Pilar; Hauel, Norbert; Kauffmann, Iris; Priepke, Henning; Niestroj, Claudia; Daiber, Christine; Enenkel, Barbara; Guilliard, Bernd; Lauritsch, Ines; Müller, Elfriede; Pascolo, Emanuelle; Sauter, Gabriele; Pantic, Milena; Martens, Uwe M.; Wenz, Christian; Lingner, Joachim; Kraut, Norbert; Rettig, Wolfgang J. // EMBO Journal;12/15/2001, Vol. 20 Issue 24, p6958 

    Telomerase, the ribonucleoprotein enzyme maintaining the telomeres of eukaryotic chromosomes, is active in most human cancers and in germline cells but, with few exceptions, not in normal human somatic tissues. Telomere maintenance is essential to the replicative potential of malignant cells and...

  • Design and study of telomerase inhibitors based on G-quadruplex ligands. Negrutska, V. V.; Dubey, L. V.; Ilchenko, M. M.; Dubey, I. Ya. // Biopolymers & Cell;2013, Vol. 29 Issue 3, p169 

    In this review we have summarized the results of our recent research on telomerase inhibitors and G-quadruplex DNA ligands. A series of potential enzyme inhibitors were synthesized and studied. These compounds were based on tricyclic heteroaromatic systems (thiazolobenzimidazoles phenazines,...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics