TITLE

Lactonas sesquiterpénicas de las plantas Viguiera sylvatica y Decachaeta thieleana (Asteraceae) modulan la producción de óxido nítrico y la fagocitosis de macrófagos RAW

AUTHOR(S)
Dupuy, L. Omar A.; Murillo, Renato; Bonilla, V. José A.
PUB. DATE
September 2008
SOURCE
Revista de Biología Tropical;sep2008, Vol. 56 Issue 3, p1063
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Different species of the Asteraceae family are a potential source of sesquiterpene lactones with anti-inflammatory properties. Macrophages play a central role in the regulation of immune responses. In the present study, the in vitro effect of two sesquiterpene lactones, a millerenolide and a thieleanin, was assessed by measuring the production of nitric oxide (NO) by cell line RAW (murine macrophages) using the Griess reagent. Additionally, the effect of these sesquiterpene lactones on phagocytic capacity of latex particles and the reduction of nitroblue tetrazolium (NBT) were evaluated microscopically. Treatment of macrophages with >2.5 μg/ml of both sesquiterpene lactones, reduced the production of NO. A decreased number of macrophages able to reduce NBT were observed when these cells were treated with 3 μg/ml of millerenolide or 7.5 μg/ml of thieleanin. Treatment of macrophages with 4 μg/ml of millerenolide or 7.5 μg/ml of thieleanin, reduced the phagocytic capacity of macrophages. Cytotoxic effects on the macrophages were only observed when the concentration was increased to 8 μg/ml of millerenolide or 25 μg/ml of thieleanin. Our results suggest that these sesquiterpene lactones could be useful compounds in the elaboration of anti-inflammatory drugs.
ACCESSION #
36619700

 

Related Articles

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics