TITLE

Characterization of lipid extracts from brain tissue and tumors using Raman spectroscopy and mass spectrometry

AUTHOR(S)
Köhler, Milena; Machill, Susanne; Salzer, Reiner; Krafft, Christoph
PUB. DATE
March 2009
SOURCE
Analytical & Bioanalytical Chemistry;Mar2009, Vol. 393 Issue 5, p1513
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Brain tissue is characterized by high lipid content. The amount of lipids decreases, and its composition changes in the most frequent primary brain tumor, the glioma. Scope of the current paper was to extract quantitatively lipids from porcine and human brain tissue as well as from five human gliomas using a modified protocol according to Folch. The lipid extracts were studied by Raman spectroscopy with 785 nm excitation and by mass spectrometry with electron impact ionization. Porcine and human brain tissues have similar water and lipid content and show similar Raman and mass spectra. In contrast, gliomas are characterized by increased water content and decreased lipid content. Elevated phosphatidylcholine to cholesterol ratios in lipid extracts of gliomas were indicated by Raman bands of the choline group and cholesterol. Due to its higher sensitivity, mass spectrometry detected increased levels of cholesterol ester relative to cholesterol in lipid extracts of gliomas. For comparison, thin tissue sections were prepared from the glioma specimens before lipid extraction; infrared spectroscopic images were recorded and analyzed by a supervised classification model. This study demonstrates how to improve the analysis of brain tumors and to complement the diagnosis of brain pathologies using a multimodal approach.
ACCESSION #
36548680

 

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