Effects of Fluoride on the Calcium Phosphate Precipitation Method for Dentinal Tubule Occlusion

Suge, T.; Ishikawa, K.; Kawasaki, A.; Yoshiyama, M.; Asaoka, K.; Ebisu, S.
April 1995
Journal of Dental Research;Apr1995, Vol. 74 Issue 4, p1079
Academic Journal
Use of the calcium phosphate precipitation (CPP) method makes possible the occlusion of dentinal tubules to approximately 10 to 15 µm from the dentinal surface, and thus shows good potential for the treatment of dentin hypersensitivity. The precipitate formed in the dentinal tubules by the CPP method is, however, not apatite [HAP; Ca10(PO4)6(OH)2], a component of tooth and bone, but dicalcium phosphate dihydrate (DCPD; CaHPO4 2H2O) Since fluoride enhances the conversion of DCPD to HAP, we evaluated the effects of fluoride on the texture of the precipitate formed by the CPP method and on its capacity to occlude dentinal tubules in this in vitro study. CPP solution (1.0 mol/L CaHPO4 2H2O dissolved in 2.0 mol/L H3PO4) was applied to a dentin disk and was subsequently neutralized with a post-treatment solution (1 mol/L NaOH, from 0 to 0.1 mol/L NaF). Scanning electron microscopy revealed that the precipitate occluded dentinal tubules to a depth of approximately 10 to 15 µm from the dentinal surface, regardless of the NaF concentration (from 0 to 0.1 mol/L) in the post-treatment solution. Also, dentin permeability was reduced to 15% by the CPP treatment regardless of the NaF concentration. The Ca/P molar ratio of the precipitate, measured by x-ray micro-analysis, was higher (1.25 ± 0.04) in the presence of NaF than in its absence (1.03 ± 0.01). For further identification of the precipitate formed in the dentinal tubules, the same procedure was used in glass tubes (diameter, 1 mm), so that a larger amount of precipitate would be obtained. Powder x-ray diffraction analysis and Fourier transform infrared (FT-IR) measurement revealed that the precipitate formed by the CPP method gradually changed from DCPD to apatitic as the NaF concentration increased. We concluded that the addition of NaF to the post-treatment solution with the CPP method was desirable, since it led to more apatitic precipitate formation and did not lessen the occluding capacity of the precipitate.


Related Articles

  • Occlusion of Dentinal Tubules with Calcium Phosphate Using Acidic Calcium Phosphate Solution Followed by Neutralization. Ishikawa, K.; Suge, T.; Yoshiyama, M.; Kawasaki, A.; Asaoka, K.; Ebisu, S. // Journal of Dental Research;Jun1994, Vol. 73 Issue 6, p1197 

    The occlusion of dentinal tubules with calcium phosphate, by a calcium phosphate precipitation method (CPP method), was investigated in vitro for evaluation of the potential value of this method for the treatment of dentin hypersensitivity. The method consists of treating the dentinal surface...

  • Constant Composition Dissolution Kinetics Studies of Human Dentin. Paschalis, E. P.; Tan, J.; Nancollas, G. H. // Journal of Dental Research;Apr1996, Vol. 75 Issue 4, p1019 

    The Constant Composition (CC) method has been used to study the dissolution kinetics of whole powdered human dentin as a function of calcium phosphate concentration at relative undersaturations with respect to hydroxyapatite (σ FHAP), ranging from +0.8 to -2.8, ionic strength from 0.05 to...

  • Scanning electron microscopic study of teeth restored with fiber posts and composite resin: An in vitro study. Sridhara, K. S.; Mankar, Sunil; Jayshankar, C. M.; Vinaya, K. // Journal of Pharmacy & Bioallied Sciences;Jul2014 Dental Supplement, Vol. 6 Issue S1, pS74 

    Aims and Objectives: The aim of this study is to compare and evaluate the thickness of resin dentin interface zones (RDIZ) obtained by luting carbon fiber post to intra-radicular dentin, either with All-Bond 2 bonding agent and C and B composite cement or Panavia F dentin-bonding system and...

  • Critical Reviews in Oral Biology & Medicine: Regulation of Dental Enamel Shape and Hardness. Simmer, J.P.; Papagerakis, P.; Smith, C.E.; Fisher, D.C.; Rountrey, A.N.; Zheng, L.; Hu, J.C.-C. // Journal of Dental Research;10/01/2010, Vol. 89 Issue 10, p1024 

    Epithelial-mesenchymal interactions guide tooth development through its early stages and establish the morphology of the dentin surface upon which enamel will be deposited. Starting with the onset of amelogenesis beneath the future cusp tips, the shape of the enamel layer covering the crown is...

  • Enamelysin (Matrix Metalloproteinase-20): Localization in the Developing Tooth and Effects of pH and Calcium on Amelogenin Hydrolysis. Fukae, M.; Tanabe, T.; Uchida, T.; Lee, S.-K.; Ryu, O.-H.; Murakami, C.; Wakida, K.; Simmer, J. P.; Yamada, Y.; Bartlett, J. D. // Journal of Dental Research;Aug1998, Vol. 77 Issue 8, p1580 

    The formation of dental enamel is a precisely regulated and dynamic developmental process. The forming enamel starts as a soft, protein-rich tissue and ends as a hard tissue that is over 95%f mineral by weight. Intact amelogenin and its proteolytic cleavage products are the most abundant...

  • Interdisciplinary Approach to a Tooth with Open Apex and Persistent Sinus. Das, Anoop N.; Geetha, Krishnamohan; Varghese Kurian, Ajay; Nair, Radhakrishnan; Nandakumar, K. // Case Reports in Dentistry;9/6/2015, Vol. 2015, p1 

    Traumatic injuries in childhood may disrupt root development leading to a tooth with open apex. Apexification procedures in such cases aim at root end closure after reasonable period of time. In some chronic cases, complete healing of the periapical area does not occur resulting in development...

  • Association between dentin thickness and presence of accessory foramina in human permanent mandibular molars. de Nazaré Silva Santana, Luana; Freitas, Luciana Brandão; Monteiro, Tamea Lacerda; de Mendonça Petta, Thais; Reis-Costa, Ana Cássia; Lima, Rafael Rodrigues // Brazilian Journal of Oral Sciences;Oct-Dec2011, Vol. 10 Issue 4, p233 

    The roots and periodontal system in human dentition are closely correlated from the early stages of dental formation, maintaining this connection after teeth are established in the oral cavity through the apical foramen or other communications. Aim: Therefore, the aim of this study was to...

  • Effect of Galla chinensis on the surface strengthening of bovine dentine in vitro. DENG Meng; LI Hong; DONG Xiao-li; SHI Ju-hon // Shanghai Journal of Stomatology;Apr2013, Vol. 22 Issue 2, p164 

    PURPOSE: To evaluate the effect of Galla chinensis extract (GCE) on strengthening the surface properties of bovine dentine in vitro. METHODS: Forty-five bovine dentine blocks were prepared and randomly divided into three groups to undergo a modified anti-acid pH-cyeling regimen. Treatments were...

  • Antiplaque Potential of Topical Stannous Fluoride. HOFFMAN, S.; TOW, H. D.; COLE, III, JOHN S. // Journal of Dental Research;Jul1977, Vol. 56 Issue 7, p709 

    Sterile enamel samples treated by topical application of 10% stannous fluoride were incubated with S mutans and S sanguis respectively and with a mixed culture to which L casei had been added. The results were compared with sterile nonfluoridated enamel samples subjected to the same procedures....


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics