Disposable screen-printed chemiluminescent biochips for the simultaneous determination of four point-of-care relevant proteins

Marquette, C. A.; Bouteille, F.; Corgier, B. P.; Degiuli, A.; Blum, L. J.
February 2009
Analytical & Bioanalytical Chemistry;Feb2009, Vol. 393 Issue 4, p1191
Academic Journal
A screen-printed (SP) microarray is presented as a platform for the achievement of multiparametric biochips. The SP platform is composed of eight (0.28-mm2) working electrodes modified with electroaddressed protein A-aryl diazonium adducts. The electrode surfaces are then used as an affinity immobilisation support for the orientated binding of capture monoclonal antibodies, having specificity against four different point-of-care related proteins (myoglobin, cardiac troponin I, C-reactive protein and brain natriuretic peptide). The immobilised capture antibodies are involved in sandwich assays of the four proteins together with biotinylated detection antibodies and peroxidase-labelled streptavidin in order to permit a chemiluminescent imaging of the SP platform and a sensitive detection of the assayed proteins. The performances of the system in pure buffered solutions, using a 25-min assay duration, were characterised by dynamic ranges of 0.5–50, 0.1–120, 0.2–20 and 0.67–67 μg/L for C-reactive protein, myoglobin, cardiac troponin I and brain natriuretic peptide, respectively. The four different assays were also validated in spiked 40-times-diluted human sera, using LowCross buffer, and were shown to work simultaneously in this complex medium. [Figure not available: see fulltext.]


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