TITLE

Green fluorescent protein based pH indicators for in vivo use: a review

AUTHOR(S)
Bizzarri, Ranieri; Serresi, Michela; Luin, Stefano; Beltram, Fabio
PUB. DATE
February 2009
SOURCE
Analytical & Bioanalytical Chemistry;Feb2009, Vol. 393 Issue 4, p1107
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Green fluorescent protein (GFP) and its variants have been used as fluorescent reporters in a variety of applications for monitoring dynamic processes in cells and organisms, including gene expression, protein localization, and intracellular dynamics. GFP fluorescence is stable, species-independent, and can be monitored noninvasively in living cells by fluorescence microscopy, flow cytometry, or macroscopic imaging techniques. Owing to the presence of a phenol group on the chromophore, most GFP variants display pH-sensitive absorption and fluorescence bands. Such behavior has been exploited to genetically engineer encodable pH indicators for studies of pH regulation within specific intracellular compartments that cannot be probed using conventional pH-sensitive dyes. These pH indicators contributed to shedding light on a number of cell functions for which intracellular pH is an important modulator. In this review we discuss the photophysical properties that make GFPs so special as pH indicators for in vivo use and we describe the probes that are utilized most by the scientific community.
ACCESSION #
36420640

 

Related Articles

  • Fluorescence microscopy today. Yuste, Rafael // Nature Methods;Dec2005, Vol. 2 Issue 12, p902 

    The article discusses the evolution and advances in fluorescence microscopy. The introduction of green fluorescent protein and two-photon microscopy has allowed systematic imaging studies of protein localization in living cells and of the structure and physiology of tissues. Advances in...

  • Sensing Phosphatidylserine in Cellular Membranes. Kay, Jason G.; Grinstein, Sergio // Sensors (14248220);2011, Vol. 11 Issue 2, p1744 

    Phosphatidylserine, a phospholipid with a negatively charged head-group, is an important constituent of eukaryotic cellular membranes. On the plasma membrane, rather than being evenly distributed, phosphatidylserine is found preferentially in the inner leaflet. Disruption of this asymmetry,...

  • Structural insights into yeast septin organization from polarized fluorescence microscopy. Vrabioiu, Alina M.; Mitchison, Timothy J. // Nature;9/28/2006, Vol. 443 Issue 7110, p466 

    Septins are polymerizing GTPases that function in cortical organization and cell division. In Saccharomyces cerevisiae they localize at the isthmus between the mother and the daughter cells, where they undergo a transition from a non-dynamic hourglass-shaped assembly to two separate rings, at...

  • Mitochondrial Dynamics and Motility Inside Living Vascular Endothelial Cells: Role of Bioenergetics. Giedt, Randy; Pfeiffer, Douglas; Matzavinos, Anastasios; Kao, Chiu-Yen; Alevriadou, B. // Annals of Biomedical Engineering;Sep2012, Vol. 40 Issue 9, p1903 

    The mitochondrial network is dynamic with conformations that vary between a tubular continuum and a fragmented state. The equilibrium between mitochondrial fusion/fission, as well as the organelle motility, determine network morphology and ultimately mitochondrial/cell function. Network...

  • Nuclear Localization Signals in p170, the Large Subunit of Translation Initiation Factor 3. Chudinova, E. M.; Ivanov, P. A.; Nadezhdina, E. S. // Molecular Biology;Jul/Aug2004, Vol. 38 Issue 4, p575 

    Apart from their role in translation, eukaryotic translation factors or their individual subunits may perform other functions, in particular, regulating nuclear processes. Primary structure analysis revealed four potential nuclear localization signals (NLS) in the human eIF3 large subunit, p170....

  • Ca2+-mediated GTP-dependent dynamic assembly of bacterial cell division protein FtsZ into asters and polymer networks in vitro. Yu, Xuan-Chuan; Margolin, William // EMBO Journal;9/1/97, Vol. 16 Issue 17, p5455 

    FtsZ, a tubulin-like GTPase that forms a dynamic ring marking the division plane of prokaryotic cells, is essential for cytokinesis. It is not known what triggers FtsZ ring assembly. In this work, we use a FtsZ—green fluorescent protein (Gfp) chimera to assay FtsZ assembly over time by...

  • Two-Photon Microscopy Imaging of thy1GFP-M Transgenic Mice: A Novel Animal Model to Investigate Brain Dendritic Cell Subsets In Vivo. Laperchia, Claudia; Allegra Mascaro, Anna L.; Sacconi, Leonardo; Andrioli, Anna; Mattè, Alessandro; De Franceschi, Lucia; Grassi-Zucconi, Gigliola; Bentivoglio, Marina; Buffelli, Mario; Pavone, Francesco S. // PLoS ONE;Feb2013, Vol. 8 Issue 2, p1 

    Transgenic mice expressing fluorescent proteins in specific cell populations are widely used for in vivo brain studies with two-photon fluorescence (TPF) microscopy. Mice of the thy1GFP-M line have been engineered for selective expression of green fluorescent protein (GFP) in neuronal...

  • Analyses of linker histone – chromatin interactions in situ. Rundquist, Ingemar; Lindner, Herbert H. // Biochemistry & Cell Biology;Aug2006, Vol. 84 Issue 4, p427 

    Recent studies, using cytometric techniques based on fluorescence microscopy, have provided new information on how linker histones interact with chromatin in vivo or in situ. In particular, the use of green fluorescent proteins (GFPs) has enabled detailed studies of how individual H1 subtypes,...

  • Identification of HrpX regulon genes in Xanthomonas oryzae pv. oryzicola using a GFP visualization technique. Li, Yurong; Xiao, Youlun; Zou, Lifang; Zou, Huasong; Chen, Gongyou // Archives of Microbiology;Apr2012, Vol. 194 Issue 4, p281 

    Xanthomonas oryzae pv. oryzicola is the causal agent of bacterial leaf streak in rice and injects repertoires of T3S effectors (T3SEs), which are normally regulated by a global regulator HrpX, into plant cells to suppress plant innate immunity for disease development. To establish a...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics