TITLE

Study on an on-line molecularly imprinted solid-phase extraction coupled to high-performance liquid chromatography for separation and determination of trace estrone in environment

AUTHOR(S)
Zhixiang Xu; Shuang Chen; Wei Huang; Guozhen Fang; Hua Pingzhu; Shuo Wang
PUB. DATE
February 2009
SOURCE
Analytical & Bioanalytical Chemistry;Feb2009, Vol. 393 Issue 4, p1273
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Estrone is one of the important potential endocrine-disrupting compounds, and the sensitive and reliable analytical methods for the determination of estrone are required for the assurance of human health. In this paper, using estrone as template molecule, 3-aminopropyltriethoxysilane as function monomer, and tetraethoxysilicane as cross-linker, a highly selective molecularly imprinted microsphere was synthesized by surface molecular imprinting technique combined with a sol–gel process. The imprinted material was characterized by the Fourier transform infrared and static adsorption experiments, and the results showed that it exhibited good recognition and selective ability for estrone. A novel method for separation and determination of trace estrone in environmental sample was developed using on-line molecularly imprinted solid-phase extraction coupled to high-performance liquid chromatography. With a sample loading flow rate of 2.6 mL min−1 for a 9.6-min extraction, the enrichment factor obtained by the slopes of the linear portion in comparison with the direct injection of 10 μL standard sample solution was 1,045. The detection limit ( S/ N = 3) was 5.7 ng L−1, and the relative standard deviations for nine replicate extractions of 5.0 μg L−1 estrone was less than 10.0%. This method was evaluated for quantitative determination of estrone in well and lake water samples spiked at two levels (0.5 and 1.0 μg L−1) with recoveries ranging from 86% to 95%. [Figure not available: see fulltext.]
ACCESSION #
36420625

 

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