TITLE

EMULSAN ANALYSIS PRODUCED BY LOCALLY ISOLATED BACTERIA AND ACINETOBACTER CALCOACETICUS RAG-1

AUTHOR(S)
Chamanrokh, P.; Assadi, M. Mazaheri; Noohi, A.; Yahyai, S.
PUB. DATE
June 2008
SOURCE
Iranian Journal of Environmental Health Science & Engineering (I;2008, Vol. 5 Issue 2, p101
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Growth of previously isolated bacteria from Iranian oil reservoirs on different carbon and energy sources and under varying conditions have been used to produce a class of extracellular microbial protein-associated lipopolysaccharides named emulsan.Several Bacteria were previously isolated from Iranian oil reservoirs and designated as; Ilam-1 and Paydar-4. In present study, the isolated strains were compared with standard sample of Acinetobacter calcoaceticus RAG-1 from Persian Type Culture Collection (PTCC 1641), IROST. Among the isolated strains, two strains were found to produce an extracellular, emulsifying agent when grown in Mineral Salt Medium containing soya oil, ethanol or local crude oil. The isolated bacteria were cultured and further analysed using protein estimation, reducing sugar analysis, hemolytic activity, surface tension and emulsification activity tests. The crude emulsifier of RAG-1, PAYDAR-4 and ILAM-1 were concentrated from the cell-free culture fluid by ammonium sulfate precipitation to yield 1.89g, 1.78g and 1.69g of bioemulsan respectively. Emulsifying activity was observed over the entire production process. These investigations showed that emulsan produced by isolated Iranian crude oil reservoir were comparable with Acinetobacter calcoaceticus RAG-1 which is made of carbohydrate backbone as its hydrophilic part (N-acetyl-Dgalactoseamine, N-acetylgalactoseamine uronic acid, diamino-6-deoxy-D-glucose) and fatty acid chain as its hydrophobic portion.
ACCESSION #
36352555

 

Related Articles

  • Reducing heavy oil carbon footprint and enhancing production through CO2 injection. Emadi, Alireza; Sohrabi, Mehran; Jamiolahmady, Mahmoud; Ireland, Shaun; Robertson, Graeme // Chemical Engineering Research & Design: Transactions of the Inst;Sep2011, Vol. 89 Issue 9, p1783 

    The world's dependence on heavy oil production is on the rise as the existing conventional oil reservoirs mature and their production decline. Compared to conventional oil, heavy oil is much more viscous and hence its production is much more difficult. Various thermal methods and particularly...

  • The fuzzy evaluation based on entropy method for heavy oil reservoir horizontal well CO2 huff and puff well selection. Yahui Li; Caizhen Peng // Applied Mechanics & Materials;2014, Issue 607-609, p886 

    Due to its high viscosity, it is very difficult to develop heavy oil cost-effectively. Currently, CO2 huff and puff method is a better choice to develop heavy oil reservoir. The key to this method is to choose the suitable candidate target wells. However, it is now very difficult to judge the...

  • Analytical and numerical investigation of multicomponent multiphase WAG displacements. LaForce, Tara; Jessen, Kristian // Computational Geosciences;Sep2010, Vol. 14 Issue 4, p745 

    In this paper the ability of analytical solutions for four-component three-phase flow to predict displacement efficiency in water alternating gas (WAG) injection processes is studied. First analytical solutions for Riemann problems with injection compositions that are the average water and gas...

  • GROWTH RATE OF MIXED RUMINAL BACTERIA AS A FUNCTION OF ENERGETIC SUBSTRATE CONCENTRATION IN BATH CULTURE. de Oliveira, Tadeu Silva; Lana, Rogério de Paula; Pereira, José Carlos; Guimarães, Geicimara; Neto, José Alípio Faleiro // Pesquisa Agropecuária Tropical;2011, Vol. 41 Issue 1, p39 

    The objective of this study was to evaluate levels of sucrose on microbial growth. Roll tubes were filled with 15.6 mL of buffer Chen, 1 mL of inocula (rumen fluid from fistulated steer at pasture), 1 mL of Trypticase or hydrolyzed casein (1 g L-1 as final concentration) and 2.4 mL of sucrose...

  • The Yersinia YopE and YopH type III effector proteins enhance bacterial proliferation following contact with eukaryotic cells. Bartra, Sara; Cherepanov, Peter; Forsberg, Åke; Schesser, Kurt // BMC Microbiology;2001, Vol. 1, p22 

    Background: Several bacterial pathogens express antihost factors that likely decrease both their maximal growth rate (due to metabolic costs) as well as their mortality rate (by neutralizing host defenses). The pathogenic yersiniae make a huge metabolic investment expressing virulence proteins...

  • 130G Grader...  // PS: Preventive Maintenance Monthly;Apr99, Issue 557, p23 

    Presents tips on how to gain accurate reading from the hydraulic oil reservoir. Set-up of 130G grader on hydraulic lines; Safety precautions.

  • Supervision system adds subsea controls to optimize production.  // Offshore;Feb98, Vol. 58 Issue 2, p42 

    Highlights the Active Reservoir Management System (ARMS), an intelligent completions technology developed by ABB Seatec, Dresser Oil Tools and Sperry-Sun Drilling Services. Features of ARMS; Performance of ARMS in ultra-long subsea tiebacks; Discussion of three completion scenarios varying in...

  • Adding molasses to or removing sulfates from injected seawater. Le Blanc, Leonard // Offshore;Sep97, Vol. 57 Issue 9, p34 

    Focuses on issues associated with the injection of seawater into oil reservoirs. Description of the fluids resident in an oil and gas reservoir; Benefits of priming of injection fluids with molasses; Issues that scientists should consider regarding injection fluids.

  • Proper oil, water atomization needed for burner combustion. Young, Tim // Offshore;Jun96, Vol. 56 Issue 6, p52 

    Discusses oil and water atomization in burner combustion in oil reservoir operations. Need for operators to atomize crude oil into fine droplets for efficient flaring; Use of a modular burner system; Use of production flow tests; Combustion process; Burner nozzle formation; Burner operation.

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics