TITLE

Multimode waveguide evanescent field fluorescence microscopy: Measurement of cell-substratum separation distance

AUTHOR(S)
Hassanzadeh, Abdollah; Armstrong, Souzan; Dixon, S. Jeffrey; Mittler, Silvia
PUB. DATE
January 2009
SOURCE
Applied Physics Letters;1/19/2009, Vol. 94 Issue 3, pN.PAG
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Waveguide evanescent field fluorescence microscopy allows imaging of thin films and contact regions between biological cells and their substratum. On changing the propagating waveguide mode exciting the fluorescence markers, the normalized evanescent field intensity at a specific distance from the waveguide surface is modified. This enables the determination of thin film thickness and cell-substratum separation distances. An extensive analysis of multimode ion-exchanged waveguides is presented, focusing on their applications as substrata for studying interfacial phenomena. The experimental results on Langmuir–Blodgett films are in excellent agreement with the simulations. For osteoblastic cells, close contacts were observed.
ACCESSION #
36339282

 

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