Surface area of lipid membranes regulates the DNA-binding capacity of cationic liposomes

Marchini, Cristina; Montani, Maura; Amici, Augusto; Pozzi, Daniela; Caminiti, Ruggero; Caracciolo, Giulio
January 2009
Applied Physics Letters;1/19/2009, Vol. 94 Issue 3, pN.PAG
Academic Journal
We have applied electrophoresis on agarose gels to investigate the DNA-binding capacity of cationic liposomes made of cationic DC-cholesterol and neutral dioleoylphosphatidylethanolamine as a function of membrane charge density and cationic lipid/DNA charge ratio. While each cationic liposome formulation exhibits a distinctive DNA-protection ability, here we show that such a capacity is universally regulated by surface area of lipid membranes available for binding in an aspecific manner. The relevance of DNA protection for gene transfection is also discussed.


Related Articles

  • The SV40 Late Protein VP4 Is a Viroporin that Forms Pores to Disrupt Membranes for Viral Release. Raghava, Smita; Giorda, Kristina M.; Romano, Fabian B.; Heuck, Alejandro P.; Hebert, Daniel N. // PLoS Pathogens;Jun2011, Vol. 7 Issue 6, Special section p1 

    Nonenveloped viruses are generally released by the timely lysis of the host cell by a poorly understood process. For the nonenveloped virus SV40, virions assemble in the nucleus and then must be released from the host cell without being encapsulated by cellular membranes. This process appears to...

  • 531. Complexes of DNA-Cationic Liposomes with Baculovirus GP64 Envelope Fusion Protein Vesicles Demonstrate Enhanced Transfection Efficiency. Guibinga, Ghiabe H.; Shaochun Song; Loring, Jeanne; Friedmann, Theodore // Molecular Therapy;Jun2006, Vol. 13, pS204 

    The need for efficient and safe gene transfer reagents has led to the growing interest in a class of hybrid vectors comprising both viral and non-viral components can be associated. We have examined the transfection efficiency of DOTAP-based lipoplexes together with partially purified vesicles...

  • THE STRUCTURE AND BINDING CAPACITY OF LIPOPLEXES. Rajnohová, Z.; Lengyel, A.; Funari, S. S.; Uhríková, D. // Acta Facultatis Pharmaceuticae Universitatis Comenianae;2010, Vol. 17, p77 

    Lipoplexes were prepared due to DNA interaction with dioleoylphosphatidylcholine liposomes (DOPC) charged either with cationic surfactant hexadecyltrimethyl-ammonium bromide (C16TMABr) or calcium divalent cations. UV-VIS spectrophotometry has shown that ~ 96 % of DNA is incorporated in...

  • Effect of membrane charge density on the protein corona of cationic liposomes: Interplay between cationic charge and surface area. Caracciolo, Giulio; Pozzi, Daniela; Candeloro De Sanctis, Sofia; Laura Capriotti, Anna; Caruso, Giuseppe; Samperi, Roberto; Laganà, Aldo // Applied Physics Letters;7/18/2011, Vol. 99 Issue 3, p033702 

    Here we investigate the effect of membrane charge density on the protein corona that forms upon exposure of cationic liposomes to human plasma. We show that the protein corona around cationic liposomes is not uniquely defined by membrane charge density, but it is deeply affected by both the...

  • 177. A Novel Class of Cationic Amphiphiles for Airway Gene Delivery. Keun-Sik Kim; Qun Li; Suda, Takeshi; Dexi Liu // Molecular Therapy;Jun2006, Vol. 13, pS68 

    A new class of cationic amphiphiles was synthesized and characterized for their transfection activity in mouse lung by intratracheal delivery. These cationic compounds, consisting of a backbone of the bis(2-hydroxy-3-alkiammonio)propyl methylamine with various alkyl chains (C12 to C18) linked to...

  • Topical DNA Vaccination with DNA/Lipid Based Complex. Myeong Jun Choi; Jong Heon Kim; Maibach, Howard I. // Current Drug Delivery;Mar2006, Vol. 3 Issue 1, p37 

    Topical DNA vaccines have been shown to elicit both broad humoral and cellular immune response in vivo. The skin is an attractive site for the delivery DNA antigens for DNA vaccination. However, due to skin's barrier properties, the penetration of DNA and the applications of topical vaccination...

  • Development of Liposomal Bubbles with Perfluoropropane Gas as Gene Delivery Carriers. Maruyama, Kazuo; Suzuki, Ryo; Sawamura, Kaori; Takizawa, Tomoko; Utoguchi, Naoki; Negishi, Yoichi // AIP Conference Proceedings;2007, Vol. 911 Issue 1, p467 

    Liposomes have some advantages as drug, antigen and gene delivery carriers. Their size can be easily controlled and they can be modified to add a targeting function. Based on liposome technology, we developed novel liposomal bubbles (Bubble liposomes) containing the ultrasound imaging gas,...

  • The nuclear localization signal sequence of porcine circovirus type 2 ORF2 enhances intracellular delivery of plasmid DNA. Chen, Hsu-Chung; Chiou, Siao-Ting; Zheng, Jin-Yi; Yang, Shu-Hsiang; Lai, Shiow-Suey; Kuo, Tsun-Yung // Archives of Virology;May2011, Vol. 156 Issue 5, p803 

    The phospholipid bilayer of the cell membrane is a natural barrier that prevents large molecules from entering the cell. Cationic liposomes are commonly used for transfection of plasmid DNA but they have high cost and toxicity. Many reports have shown that cell-penetrating peptides (CPP) are...

  • Modeling of Cationic Lipid-DNA Complexes. May, S.; Ben-Shaul, A. // Current Medicinal Chemistry;Jan2004, Vol. 11 Issue 2, p151 

    Cationic lipid-DNA complexes, often referred to as lipoplexes, are formed spontaneously in aqueous solutions upon mixing DNA and liposomes composed of cationic and nonionic lipids. Understanding the mechanisms underlying lipoplex formation, structure and phase behavior is crucial for their...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics