TITLE

Improved Performances of Polyacrylamide Gel by Addition of a New Type of Polymer: Detection of Allelic Losses

AUTHOR(S)
Korolija, Marina; Hadžija, Mirko; Pavković, Pajica; Kapitanović, Sanja; Hadžija, Marijana Popović
PUB. DATE
November 2008
SOURCE
Croatica Chemica Acta;Nov2008, Vol. 81 Issue 3, p437
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The aim of this study was to improve detection of heterozygous samples and loss of heterozygosity using a new type of gel polymer. A total of 60 samples of normal and tumor colon tissue were tested. Elctrophoresis of amplified loci was performed on a standard acrylamide / N,N-methylenebisacrylamide gel, and the same gel containing Spreadex polymer, NAB (native acrylamide-bis). Standard gel displayed 36 samples of normal tissue as homozygous. Electrophoresis of the same samples on gel with addition of polymer revealed 12/36 heterozygous samples. Five of 12 matched tumor samples displayed loss of heterozygosity that could not be detected on standard gel, because of its lower resolving power. Electrophoresis on a new polymer presents a simple, inexpensive, reproducible and non-radioactive method for loss of heterozygosity detection, with improved performances such as increased sensitivity, decreased amount of a sample and improved separation of closely spaced bands. Application of the polymer may contribute to a vide variety of diagnostic procedures.
ACCESSION #
36330425

 

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