TITLE

Effect of Constant Glucose Feeding on the Production of Exopolysaccharides by Tremella fuciformis Spores

AUTHOR(S)
H. Zhu; S. Sun
PUB. DATE
March 2009
SOURCE
Applied Biochemistry & Biotechnology;Mar2009, Vol. 152 Issue 3, p366
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Abstract  A fed-batch culture system with constant feeding (glucose 80 g L−1, 0.25 ml min−1) was used to study the influence of glucose on cell dry weight and exopolysaccharides production from submerged Tremella fuciformis spores in a 5-L stirred-tank bioreactor. The results showed that high levels of cell mass (9.80 g L−1) and exopolysaccharides production (3.12 g L−1) in fed-batch fermentation were obtained after 1 h of feeding, where the specific growth rate (μ) and exopolysaccharides yield on substrate consumed (YP/S) were 0.267 d−1 and 0.14 g g−1. Unlike batch fermentation, maximal cell mass and exopolysaccharides production merely reached 7.11 and 2.08 g L−1; the specific growth rate (μ) and exopolysaccharides yield on substrate consumed (YP/S) were 0.194 d−1 and 0.093 g g−1, respectively. It is concluded that the synthesis of exopolysaccharides can be promoted effectively when feeding glucose at a late exponential phase.
ACCESSION #
36296797

 

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