Gene and protein expression of p53 and p21 in fibroadenomas and adjacent normal mammary tissue

Rodrigo Cericatto; Edison Capp
February 2009
Endocrine (1355008X);Feb2009, Vol. 35 Issue 1, p118
Academic Journal
Abstract  The aim of this study was to compare p53 and p21 mRNA, and proteins levels between fibroadenomas and adjacent normal mammary tissue of women in reproductive age. A transversal study was performed. Fourteen patients who attended the Breast Service of the Hospital de Clínicas de Porto Alegre were assessed and submitted to surgical resection of fibroadenomas. Fragments of the central area of the fibroadenoma and adjacent normal mammary tissue were obtained. mRNA expression for genes p53 and p21 was evaluated by RT-PCR, and protein expression by the western blot. Paired analyses showed higher gene expression of p53 (P = 0.017) and p21 (P = 0.003), and a higher protein expression of p53 (P = 0.001) in fibroadenomas as compared to normal breast tissue. p21 protein expression was not different (P = 0.97) between the fibroadenoma and the adjacent normal mammary tissue samples. These results suggest the participation of p53 in the formation of fibroadenomas. The role of p21 in fibroadenomas remains to be defined.


Related Articles

  • Discovery of TP53 splice variants in two novel papillary urothelial cancer cell lines. Koch, Annemarie; Hatina, Jiri; Rieder, Harald; Seifert, Hans-Helge; Huckenbeck, Wolfgang; Jankowiak, Frank; Florl, Andrea; Stoehr, Robert; Schulz, Wolfgang // Cellular Oncology (2211-3428);Aug2012, Vol. 35 Issue 4, p243 

    Background: Using a novel cell culture technique, we established two new cell lines, BC44 and BC61, from papillary urothelial carcinoma and analyzed them for genetic changes typical of this tumor type. Methods and results: Karyotyping revealed aneuploid karyotypes with loss of chromosome 9 and...

  • Differential protein and mRNA expression of CaMKs during osteoclastogenesis and its functional implications. Yao, Chao Hua; Zhang, Pei; Zhang, Liang // Biochemistry & Cell Biology;Aug2012, Vol. 90 Issue 4, p532 

    The calmodulin-dependent kinase (CaMK) family has been recently recognized to participate in the regulation of osteoclastogenesis. However, there are some controversial reports regarding the mRNA expression patterns of CaMKs during osteoclastogenesis, although the protein expression pattern of...

  • Regulation of the levels of Smad1 and Smad5 in MC3T3-E1 cells by Icariine in vitro. HUIFANG ZHOU; SHUO WANG; YUAN XUE; NIANKE SHI // Molecular Medicine Reports;2014, Vol. 9 Issue 2, p590 

    The purpose of this study was to investigate the role of Icariine on the expression of Smadl and Smad5 mRNA and protein levels in MC3T3-E1 cells in vitro. MC3T3-E1 cells were cultured in the presence of different concentrations of Icariine (0, 10, 40 and 80 ng/ml). Smad1 and Smad5 mRNA levels...

  • The expression of chitinase 3-like 1: a novel prognostic predictor for hepatocellular carcinoma. Pan, Jing-Jing; Ge, Yong-Sheng; Xu, Ge-Liang; Jia, Wei-Dong; Liu, Wei-Feng; Li, Jian-Sheng; Liu, Wen-Bin // Journal of Cancer Research & Clinical Oncology;Jun2013, Vol. 139 Issue 6, p1043 

    Objectives: Chitinase 3-like 1 (CHI3L1) is associated with poor prognosis of various human cancers. However, the clinical and prognostic significance of CHI3L1 in hepatocellular carcinoma (HCC) is largely unknown. The aim of the present study is to investigate the expression of CHI3L1 in human...

  • Clinicopathological Significance of NMIIA Overexpression in Human Gastric Cancer. Dongning Liu; Lei Zhang; Zhiyong Shen; Fei Tan; Yanfeng Hu; Jiang Yu; Guoxin Li // International Journal of Molecular Sciences;Nov2012, Vol. 13 Issue 11, p15291 

    Altered expressions of nonmuscle myosin IIA (NMIIA) have been observed in certain types of cancers, but the impact of the alterations in gastric cancer (GC) remains unclear. The purpose of this study was to evaluate the expression of NMIIA at the mRNA and protein level in patients with GC and to...

  • Upregulation of the constitutively expressed HSC70 by KLF4. Liu, Ying; Zhao, Jie; Liu, Junwen; Zhang, Huali; Liu, Meidong; Xiao, Xianzhong // Cell Stress & Chaperones;Autumn2008, Vol. 13 Issue 3, p337 

    Krüppel-like factor 4 (KLF4) is a transcription factor that is abundantly expressed in various organisms from bacteria to mammals. It has been demonstrated that KLF4 regulates the expression of a wide range of genes. Analysis of KLF4 target genes reveals its diverse regulatory functions in...

  • Knockdown of IRX2 inhibits osteosarcoma cell proliferation and invasion by the AKT/MMP9 signaling pathway. TIELONG LIU; WEIWEI ZHOU; FEI ZHANG; GUODONG SHI; HONGLIN TENG; JIANRU XIAO; YAN WANG // Molecular Medicine Reports;2014, Vol. 10 Issue 1, p169 

    Osteosarcoma is the most common type of human primary malignant bone tumor with a potential propensity for local invasion and distant metastasis. Thus, this study focused on the expression and roles of IRX2 in the development of osteosarcoma. The mRNA expression levels of IRX2 in tissue samples...

  • Molecular Responses to Moderate Endurance Exercise in Skeletal Muscle. Pasiakos, Stefan M.; McClung, Holly L.; McClung, James P.; Urso, Maria L.; Pikosky, Matthew A.; Cloutier, Gregory J.; Fielding, Roger A.; Young, Andrew J. // International Journal of Sport Nutrition & Exercise Metabolism;Aug2010, Vol. 20 Issue 4, p282 

    This study examined alterations in skeletal-muscle growth and atrophy-related molecular events after a single bout of moderate-intensity endurance exercise. Muscle biopsies were obtained from 10 men (23 ± 1 yr, body mass 80 ± 2 kg, and VO2peak 45 ± 1 ml · kg-1 · min-1) immediately...

  • Expression of β-catenin in the apical papilla and dental pulp tissues of human permanent teeth. Tang Ling-ling; Bao Zhi-fan; Chen Xu; Guo Yan // Shanghai Journal of Stomatology;Jun2012, Vol. 21 Issue 3, p270 

    PURPOSE: The expression of β-catenin at the level of mRNA and protein in the apical papilla and dental pulp tissues of human permanent teeth were compared to explore the possible relationship between β-catenin and the development of pulp-dentin complex. METHODS: Forty-eight healthy...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics