Effect of mutant p27kip1 gene on human cholangiocarcinoma cell line, QBC939

Jian Luo; Yong-Jun Chen; Wei-Yu Wang; Sheng-Quan Zou; Paumgartner, Gustav
September 2008
World Journal of Gastroenterology;9/14/2008, Vol. 14 Issue 34, p5344
Academic Journal
AIM: To investigate the effects of exogenously mutated p27kip1 (p27) on proliferation and apoptosis of human cholangiocarcinoma cell line, QBC939 in vivo. METHODS: Adenviral vectors were used to transfect mutated p27 cDNA into human QBC939 cell line. Expression of p27 was detected by RT-PCR. Western blot. Cell growth, morphological change, cell cycle, apoptosis and cloning formation were determined by MTT assay and flow cytometry. RESULTS: The expression of p27 protein and mRNA was increased significantly in QBC939 cell line transfected with Ad-p27mt. The transfer of Ad-p27mt could significantly inhibit the growth of QBC939 cells, decrease the cloning formation rate and induce apoptosis. p27 over expression caused cell cycle arrest at G0/G1 phase 72 h after infection with Ad-p27mt. CONCLUSION: p27 may cause cell cycle arrest at G0/G1 phase and subsequently lead to apoptosis. Recombinant adenovirus expressing mutant p27 may be potentially useful in gene therapy for cholangiocarcinoma.


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