Anti-HBc screening in Indian blood donors: Still an unresolved issue

Dhawan, Hari Krishan; Marwaha, Neelam; Sharma, Ratti Ram; Chawla, Yogesh; Thakral, Beenu; Saluja, Karan; Sharma, Sanjeev Kumar; Thakur, Manish K.; Jain, Ashish; Amarapurkar, Deepak Narayan
September 2008
World Journal of Gastroenterology;9/14/2008, Vol. 14 Issue 34, p5327
Academic Journal
AIM: To study the seroprevalence of antibody to hepatitis B core antigen (anti-HBc) in healthy blood donors negative for HBsAg and to evaluate whether anti-HBc detection could be adopted in India as a screening assay for HBV in addition to HBsAg. METHODS: A total of 1700 serum samples collected from HBsAg-negative healthy blood donors were tested for the presence of anti-HBc antibody (IgM + IgG). All samples reactive for anti-HBc antibody were then investigated for presence of anti-HBs and for liver function tests (LFTs). One hundred serum samples reactive for anti-HBc were tested for HBV DNA by PCR method. RESULTS: Out of 1700 samples tested, 142 (8.4%) blood samples were found to be reactive for anti-HBc. It was significantly lower in voluntary (6.9%) as compared to replacement donors (10.4%, P = 0.011). Seventy-two (50.7%) anti-HBc reactive samples were also reactive for anti-HBs with levels > 10 mIU/mL and 70 (49.3%) samples were non-reactive for anti-HBs, these units were labeled as anti-HBc-only. These 142 anti-HBc reactive units were also tested for liver function test. HBV DNA was detected in only 1 of 100 samples tested. CONCLUSION: Keeping in view that 8%-18% of donor population in India is anti-HBc reactive, inclusion of anti-HBc testing will lead to high discard rate. Anti-HBs as proposed previously does not seem to predict clearance of the virus. Cost effectiveness of introducing universal anti-HBc screening and discarding large number of blood units versus considering ID NAT (Individual donor nuclic acid testing) needs to be assessed.


Related Articles

  • Naturally occurring hepatitis B virus surface antigen mutant variants in Malaysian blood donors and vaccinees. Hudu, S.; Harmal, N.; Saeed, M.; Alshrari, A.; Niazlin, M.; Sekawi, Z.; Malik, Y.; Hassan, R. // European Journal of Clinical Microbiology & Infectious Diseases;Jul2015, Vol. 34 Issue 7, p1349 

    Hepatitis B virus surface mutants are of enormous importance because they are capable of escaping detection by serology and can infect both vaccinated and unvaccinated populations, thus putting the whole population at risk. This study aimed to detect and characterise hepatitis B-escaped mutants...

  • Lack of correlation between p53 codon 72 polymorphism and anal cancer risk. Contu, Simone S.; Agnes, Grasiela; Damin, Andrea P.; Contu, Paulo C.; Rosito, Mário A.; Alexandre, Claudio O.; Damin, Daniel C. // World Journal of Gastroenterology;9/28/2009, Vol. 15 Issue 36, p4566 

    AIM: To investigate the potential role of p53 codon 72 polymorphism as a risk factor for development of anal cancer. METHODS: Thirty-two patients with invasive anal carcinoma and 103 healthy blood donors were included in the study. p53 codon 72 polymorphism was analyzed in blood samples through...

  • Initial virological response and viral mutation with adefovir dipivoxil added to ongoing Lamivudine therapy in Lamivudine-resistant chronic hepatitis B. Wu, Shuang; Fukai, Kenichi; Imazeki, Fumio; Arai, Makoto; Kanda, Tatsuo; Yonemitsu, Yutaka; Yokosuka, Osamu // Digestive Diseases & Sciences;Apr2011, Vol. 56 Issue 4, p1207 

    Background: Although adefovir dipivoxil (ADV) has been used for antiviral treatment of lamivudine (LAM)-resistant chronic hepatitis B (CHB) patients, the long-term efficacy of this treatment is not well understood. Initial virological response (IVR) has been reported to be an...

  • Influence of Hepatitis B Virus (HBV) Genotype on the Clinical Course of Disease in Patients Coinfected with HBV and Hepatitis Delta Virus. Kiesslich, Dagmar; Crispim, Myuki Alfaia; Santos, Carlos; Ferreira, Fernando de Lima; Fraiji, Nelson Abrahim; Komninakis, Shirley Vasconcelos; Diaz, Ricardo Sobhie // Journal of Infectious Diseases;6/1/2009, Vol. 199 Issue 11, p1608 

    Objective. We evaluated the influence of hepatitis B virus (HBV) genotype on the course of disease in patients coinfected with HBV and hepatitis delta virus (HDV). Methods. We evaluated HBV genotypes in 190 patients, 140 of whom had chronic HBV monoinfection and 50 of whom had chronic HBV-HDV...

  • Molecular sleuthing: Tracking ovarian cancer progression. Liu, Edison; Nuzum, Christine // JNCI: Journal of the National Cancer Institute;8/2/95, Vol. 87 Issue 15, p1099 

    Editorial. Asserts the use of the polymerase chain reaction (PCR) techniques in the detection of cancer-associated molecular mutations in stool, saliva and urine and the use in cancer screening. Unlocking of the genetic and epigenetic events leading to colon tumor formation; Factors in...

  • New DNA test speeds diagnosis.  // USA Today Magazine;Oct95, Vol. 124 Issue 2605, p8 

    Reports on a study by the American Lung Association on the ability of a new DNA test to confirm the diagnosis of tuberculosis within six hours in a clinical laboratory. Comments from Daniel Chin of the San Francisco General Hospital; Public health implications; Use of a process called polymerase...

  • Polymerase chain reaction may help rule out M pneumoniae. Safrin, Sharon // Geriatrics;Mar97, Vol. 52 Issue 3, p81 

    Presents an abstract of the article `Detection of Mycoplasma Pneumoniae by Polymerase Chain Reaction in Lung Aspirates from Patients with Community-Acquired Pneumonia,' by M. Falguera, N. Nogues et al published in the `Chest' journal dated 1996.

  • Use of polymerase chain reaction in human African trypanosomiasis stage determination and follow-up. Truc, P.; Jamonneau, V.; Cuny, G.; Frézil, J.L. // Bulletin of the World Health Organization;1999, Vol. 77 Issue 9, p745 

    Investigates the use of polymerase chain reaction (PCR) in the stage determination of human African trypanosomiasis. Demonstration of the absence or presence of trypanosome using the double centrifugation technique; Assessment of the disease stage of trypanosomiasis; Evaluation of the PCR method.

  • Enzyme Immunoassay for the Diagnosis of Cat-Scratch Disease Defined by Polymerase Chain Reaction. Giladi, Michael; Kletter, Yehudith; Avidor, Boaz; Metzkor-Cotter, Einat; Varon, Merav; Golan, Yoav; Weinberg, Miriam; Riklis, Irena; Ephros, Moshe; Slater, Leonard // Clinical Infectious Diseases;12/1/2001, Vol. 33 Issue 11, p1852 

    Whole-cell immunofluorescent antibody (IFA) tests for detection of anti--Bartonella henselae immunoglobulin (Ig) G are commonly used to diagnose cat-scratch disease (CSD). The need to cultivate B. henselae in Vero cells for antigen preparation and the absence of routinely applied IFA assays...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics